##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062584_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	464285
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.069543491605373	32.0	32.0	32.0	32.0	32.0
2	30.84660284092745	32.0	32.0	32.0	32.0	32.0
3	30.829904046006224	32.0	32.0	32.0	32.0	32.0
4	30.863204712622636	32.0	32.0	32.0	32.0	32.0
5	30.723551267001948	32.0	32.0	32.0	32.0	32.0
6	34.36352671311802	36.0	36.0	36.0	32.0	36.0
7	34.197406765241176	36.0	36.0	36.0	32.0	36.0
8	34.20077753965775	36.0	36.0	36.0	32.0	36.0
9	34.304149390999065	36.0	36.0	36.0	32.0	36.0
10	34.06319825107423	36.0	36.0	36.0	32.0	36.0
11	34.37024887730597	36.0	36.0	36.0	32.0	36.0
12	34.166195332608204	36.0	36.0	36.0	32.0	36.0
13	34.2306600471693	36.0	36.0	36.0	32.0	36.0
14	34.11743218066489	36.0	36.0	36.0	32.0	36.0
15	34.09123706344164	36.0	36.0	36.0	32.0	36.0
16	34.04422499111537	36.0	36.0	36.0	32.0	36.0
17	33.96506025393885	36.0	36.0	36.0	32.0	36.0
18	33.964534714668794	36.0	36.0	36.0	32.0	36.0
19	33.964847022841575	36.0	36.0	36.0	32.0	36.0
20	33.956487933058355	36.0	36.0	36.0	32.0	36.0
21	33.83336958979937	36.0	36.0	36.0	32.0	36.0
22	33.76668856413625	36.0	36.0	36.0	32.0	36.0
23	33.599765230408046	36.0	36.0	36.0	27.0	36.0
24	33.59853538236213	36.0	36.0	36.0	27.0	36.0
25	32.9567830104354	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	26.0
5	38.0
6	80.0
7	22.0
8	60.0
9	96.0
10	47.0
11	23.0
12	40.0
13	23.0
14	107.0
15	149.0
16	224.0
17	291.0
18	387.0
19	563.0
20	772.0
21	1064.0
22	1559.0
23	2284.0
24	3048.0
25	4101.0
26	5625.0
27	7031.0
28	9641.0
29	13064.0
30	17221.0
31	23685.0
32	34255.0
33	48329.0
34	102178.0
35	188252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.86635735256423	15.794189805989406	11.969981884877427	24.36947095656894
2	14.371347768867752	21.38061606260137	35.89175287709118	28.3562832914397
3	20.899190543270826	25.872244551567864	26.502492127290196	26.726072777871117
4	13.156148755773076	16.118296339698077	33.387330254359966	37.33822465016888
5	13.817978109109713	37.31125570973024	33.31659915539085	15.554167025769198
6	34.7376858781118	31.575953335732336	18.235394252296473	15.450966533859392
7	29.104558033674444	30.841300768149804	21.217228625865353	18.8369125723104
8	30.111321413635366	30.558849017638106	21.34034086254694	17.98948870617959
9	27.40614415412776	16.51858575361722	17.55346328232968	38.52180680992534
10	16.24447688648425	27.65772545839955	31.711998067853703	24.3857995872625
11	36.52503757631172	22.35990019904124	18.8261095524699	22.28895267217714
12	22.838286675581244	24.644782814993746	28.556269680369233	23.960660829055776
13	32.17982558289988	19.984645774568918	23.920459936685383	23.91506870584582
14	25.622240865066303	18.56510965371037	23.841945105211057	31.970704376012264
15	27.012066794321104	24.137685659576672	26.343390432817543	22.50685711328469
16	27.266238708967922	25.101940041358223	24.841456551224468	22.790364698449388
17	24.65117683339731	24.706816015458205	27.68955061655977	22.952456534584712
18	27.024415703172682	22.239188055478273	28.91689689158617	21.819499349762875
19	23.747039869560155	30.336106905461307	24.462216010800972	21.45463721417757
20	23.535373793180785	22.294751778555547	31.000886312616043	23.168988115647622
21	24.37096033281358	27.096744974476096	22.553339242542858	25.97895545016746
22	22.774974707655005	27.167106361591014	24.70560573278772	25.352313197966257
23	22.1508052740218	25.98747711002931	23.990630479842807	27.87108713610608
24	21.50171679003399	29.133668064254532	24.58719395413841	24.77742119157306
25	26.22311941009897	24.53204966452506	22.66154234360139	26.58328858177458
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	126.0
1	126.0
2	278.5
3	431.0
4	431.0
5	431.0
6	1173.0
7	1915.0
8	1915.0
9	1915.0
10	1835.0
11	1755.0
12	1755.0
13	1755.0
14	1502.0
15	1249.0
16	1249.0
17	1249.0
18	1870.0
19	2491.0
20	2491.0
21	2491.0
22	3901.5
23	5312.0
24	5312.0
25	5312.0
26	7695.5
27	10079.0
28	10079.0
29	10079.0
30	12271.0
31	14463.0
32	14463.0
33	14463.0
34	17806.0
35	21149.0
36	21149.0
37	21149.0
38	25277.0
39	29405.0
40	29405.0
41	29405.0
42	40260.0
43	51115.0
44	51115.0
45	51115.0
46	79985.5
47	108856.0
48	108856.0
49	108856.0
50	88755.5
51	68655.0
52	68655.0
53	68655.0
54	56889.5
55	45124.0
56	45124.0
57	45124.0
58	40669.5
59	36215.0
60	36215.0
61	36215.0
62	32036.0
63	27857.0
64	27857.0
65	27857.0
66	23017.5
67	18178.0
68	18178.0
69	18178.0
70	13866.5
71	9555.0
72	9555.0
73	9555.0
74	7341.0
75	5127.0
76	5127.0
77	5127.0
78	4089.5
79	3052.0
80	3052.0
81	3052.0
82	2137.5
83	1223.0
84	1223.0
85	1223.0
86	868.0
87	513.0
88	513.0
89	513.0
90	353.0
91	193.0
92	193.0
93	193.0
94	124.0
95	55.0
96	55.0
97	55.0
98	123.5
99	192.0
100	192.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0068923182958743005
2	0.003015389254445007
3	0.004953853775159654
4	0.013138481751510386
5	0.03553851621310187
6	0.043938529136198674
7	0.06741548833152052
8	0.08680013353866697
9	0.09950784539668524
10	0.1188924906038317
11	0.12104634007129242
12	0.13461559171629495
13	0.12255403469851492
14	0.12880019815415103
15	0.11480017661565634
16	0.11458479166891027
17	0.12556942395295992
18	0.13095404762161172
19	0.1341848218228028
20	0.12190787985827671
21	0.12966173794113528
22	0.15206177240272678
23	0.14086175517193103
24	0.13547713150327925
25	0.13224635730208814
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	464285.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.91216919447853
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.4792733413838	41.482865501996955
2	11.779585483329265	11.994484983361655
3	3.081479845355532	4.7065446976834915
4	1.205358886660953	2.4546974231100283
5	0.6070437907187863	1.5452958090766233
6	0.3703120642942825	1.1312034283264274
7	0.2470046636785148	0.8802880260318068
8	0.16483446713383182	0.6713664223839485
9	0.124016552671304	0.5682556541265649
>10	0.7641541771425958	7.501293272126919
>50	0.10286034644623705	3.705874833215498
>100	0.05545146248699191	5.098056237146636
>500	0.010159046562502336	3.754148615619479
>1k	0.00677269770833489	6.930735905759039
>5k	8.465872135418613E-4	2.428990186374693
>10k+	8.465872135418613E-4	5.145899003660272
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	13188	2.8404966776871965	No Hit
TCCATGTACTCTGCGTTGATACCAC	10690	2.3024650807155087	No Hit
GAGTACATGGAAGCAGTGGTATCAA	6168	1.3284943515297716	No Hit
CATGTACTCTGCGTTGATACCACTG	5103	1.099109383245205	No Hit
GCTTCCATGTACTCTGCGTTGATAC	3505	0.7549242383449821	No Hit
GCGTTGATACCACTGCTTCCATGTA	3175	0.6838472059187783	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2927	0.6304317391257525	No Hit
CCCATGTACTCTGCGTTGATACCAC	2814	0.6060932401434463	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2524	0.5436316055870855	No Hit
GTATCAACGCAGAGTACATGGAAGC	2375	0.5115392485219208	No Hit
CAGTGGTATCAACGCAGAGTACATG	2018	0.4346468225335731	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	2000	0.43076989349214384	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1983	0.4271083493974606	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1746	0.37606211701864156	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1411	0.30390815985870745	No Hit
TATCAACGCAGAGTACATGGAAGCA	1372	0.29550814693561067	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1119	0.24101575540885445	No Hit
GTATCAACGCAGAGTACATGGGAAG	1074	0.23132343280528123	No Hit
GGTATCAACGCAGAGTACATGGAAG	1068	0.23003112312480484	No Hit
ATACCACTGCTTCCATGTACTCTGC	1049	0.22593880913662945	No Hit
GTGGTATCAACGCAGAGTACATGGA	961	0.2069849338229751	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	956	0.20590800908924478	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	953	0.20526185424900653	No Hit
AAAAAGTACTCTGCGTTGATACCAC	953	0.20526185424900653	No Hit
GAGTACATGGGAAGCAGTGGTATCA	936	0.2016003101543233	No Hit
GTACATGGGGTGGTATCAACGCAAA	835	0.17984643053297006	No Hit
GTACTCTGCGTTGATACCACTGCTT	823	0.1772618111720172	No Hit
CCACTGCTTCCATGTACTCTGCGTT	813	0.17510796170455645	No Hit
GCGTTGATACCACTGCTTCCCATGT	788	0.16972333803590467	No Hit
GCTTCCCATGTACTCTGCGTTGATA	756	0.1628310197400304	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	738	0.15895409069860109	No Hit
ACATGGAAGCAGTGGTATCAACGCA	737	0.158738705751855	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	729	0.15701562617788642	No Hit
GGTATCAACGCAGAGTACTTTTTTT	713	0.15356946702994928	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	673	0.1449540691601064	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	614	0.13224635730208814	No Hit
GTTGATACCACTGCTTCCATGTACT	609	0.1311694325683578	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	607	0.13073866267486564	No Hit
TATCAACGCAGAGTACATGGGAAGC	578	0.12449249921922957	No Hit
GATACCACTGCTTCCATGTACTCTG	549	0.11824633576359349	No Hit
CCCCATGTACTCTGCGTTGATACCA	537	0.11566171640264061	No Hit
GTACATGGGTGGTATCAACGCAAAA	536	0.11544633145589454	No Hit
GGTATCAACGCAGAGTACATGGGAA	525	0.11307709704168777	No Hit
GCAGAGTACATGGAAGCAGTGGTAT	501	0.10790785831978203	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.1538494674607192E-4	0.0
12	0.0	0.0	0.0	4.3076989349214383E-4	2.1538494674607192E-4
13	0.0	0.0	0.0	4.3076989349214383E-4	2.1538494674607192E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCACT	25	0.006018132	19.004208	17
TGGATAG	25	0.006025637	19.000109	5
TCTAGAT	35	0.0021748494	16.277029	2
GTCTTAG	65	1.923454E-7	16.063154	1
CGACGGT	50	8.692517E-5	15.200088	7
GGTTCTA	45	6.743865E-4	14.779456	13
AGACGGA	45	6.7493843E-4	14.777862	6
TCCTATT	45	6.777037E-4	14.769897	2
GCCGGTC	65	3.355848E-6	14.617044	8
GACGGTA	60	2.5578589E-5	14.251618	8
GACTTTC	40	0.0052677416	14.251617	14
TATACTG	40	0.0052715135	14.250082	5
TACGACG	60	2.5604628E-5	14.250081	5
CTACGAC	60	2.563069E-5	14.248545	4
CTATTCC	55	1.9571827E-4	13.816771	4
CGGTTCT	50	0.0014967169	13.3000765	12
GGGTAGG	65	5.4803008E-5	13.142581	1
CGGTCCA	60	4.0784993E-4	12.668105	10
ACGGTAT	60	4.0784993E-4	12.668105	9
CGCCGGT	60	4.0822133E-4	12.6667385	7
>>END_MODULE