FastQCFastQC Report
Thu 2 Feb 2017
SRR4062584_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062584_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464285
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA46390.9991707679550276No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC40160.8649859461322249No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA18830.4055698547228534No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC18470.3978159966399948No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG12950.2789235060361631No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC10760.2317542026987734No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA10700.23046189301829692No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG9940.21409263706559548No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA8320.17920027569273184No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8240.17747719611876325No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7960.17144641760987325No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG6560.14129252506542317No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC6560.14129252506542317No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA6440.1387079057044703No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA6150.13246174224883422No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG5940.12793865836716672No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA5470.11781556587010134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900.10553862390557524No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4730.10187707981089202No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTA250.002351361835.1998312
TGGTATA3300.025.33320844
TCGTATA1051.10958354E-1025.14273344
GTACTCG901.5075784E-824.44432335
CGAACGT551.5916102E-423.99988426
CTTACAC752.0598E-623.466553
GAACTAT602.865005E-422.0046331
ATTAGAG1205.9480953E-1021.9998933
CGCGCTA500.0025781421.99989316
CATTCGA602.8691828E-421.99989322
TTAGTGT602.8691828E-421.9998934
TATAGAG500.0025781421.9998932
ACGAAAA856.1003375E-620.70578223
GAACGTC654.926036E-420.30759227
ACCGACC550.004479651319.9999038
AGTATCC550.004479651319.9999038
TATTAGA550.004479651319.9999032
AACTATA550.004479651319.9999032
CTCGTAT909.998255E-619.55545843
ATGGGTG2850.019.2981535
Produced by FastQC (version 0.11.5)