##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062584_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	464285
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.057910550631615	32.0	32.0	32.0	32.0	32.0
2	31.390243061912404	32.0	32.0	32.0	32.0	32.0
3	31.49646660994863	32.0	32.0	32.0	32.0	32.0
4	31.62793758144243	32.0	32.0	32.0	32.0	32.0
5	31.56365163638713	32.0	32.0	32.0	32.0	32.0
6	35.17207534165437	36.0	36.0	36.0	36.0	36.0
7	35.179183044896995	36.0	36.0	36.0	36.0	36.0
8	35.149877769042725	36.0	36.0	36.0	36.0	36.0
9	35.23239604984008	36.0	36.0	36.0	36.0	36.0
10	35.146168840259755	36.0	36.0	36.0	36.0	36.0
11	35.22672496419225	36.0	36.0	36.0	36.0	36.0
12	35.14768945798378	36.0	36.0	36.0	36.0	36.0
13	35.15938486059209	36.0	36.0	36.0	36.0	36.0
14	35.11939433752975	36.0	36.0	36.0	36.0	36.0
15	35.09034752361158	36.0	36.0	36.0	36.0	36.0
16	35.06010316938949	36.0	36.0	36.0	36.0	36.0
17	35.04467729950354	36.0	36.0	36.0	36.0	36.0
18	35.02878404428314	36.0	36.0	36.0	36.0	36.0
19	34.99682522588496	36.0	36.0	36.0	36.0	36.0
20	34.98884521360802	36.0	36.0	36.0	36.0	36.0
21	34.89730445739147	36.0	36.0	36.0	32.0	36.0
22	34.849202537234675	36.0	36.0	36.0	32.0	36.0
23	34.709312168172566	36.0	36.0	36.0	32.0	36.0
24	34.69175183346436	36.0	36.0	36.0	32.0	36.0
25	34.629415122177114	36.0	36.0	36.0	32.0	36.0
26	34.53906759856554	36.0	36.0	36.0	32.0	36.0
27	34.450938539905444	36.0	36.0	36.0	32.0	36.0
28	34.373320266646566	36.0	36.0	36.0	32.0	36.0
29	34.296167224872654	36.0	36.0	36.0	32.0	36.0
30	34.2659142552527	36.0	36.0	36.0	32.0	36.0
31	34.20857232088049	36.0	36.0	36.0	32.0	36.0
32	34.1375340577447	36.0	36.0	36.0	32.0	36.0
33	34.061035786208905	36.0	36.0	36.0	32.0	36.0
34	33.99814984330745	36.0	36.0	36.0	32.0	36.0
35	33.815425869885956	36.0	36.0	36.0	32.0	36.0
36	33.77017349257461	36.0	36.0	36.0	32.0	36.0
37	33.596211378786734	36.0	36.0	36.0	27.0	36.0
38	33.49838568982414	36.0	36.0	36.0	21.0	36.0
39	33.142815296638915	36.0	36.0	36.0	14.0	36.0
40	32.94021775418116	36.0	36.0	36.0	14.0	36.0
41	32.98578459351476	36.0	36.0	36.0	14.0	36.0
42	32.601481848433615	36.0	36.0	36.0	14.0	36.0
43	32.741764218098794	36.0	36.0	36.0	14.0	36.0
44	32.74250945001454	36.0	36.0	36.0	14.0	36.0
45	32.31663310251246	36.0	36.0	36.0	14.0	36.0
46	32.67076041655449	36.0	36.0	36.0	14.0	36.0
47	32.42216526486964	36.0	36.0	36.0	14.0	36.0
48	32.50786908902937	36.0	36.0	36.0	14.0	36.0
49	32.67611057863166	36.0	36.0	36.0	14.0	36.0
50	31.95938916829103	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	8.0
21	26.0
22	91.0
23	264.0
24	775.0
25	1864.0
26	4201.0
27	7736.0
28	12291.0
29	15990.0
30	19790.0
31	24714.0
32	35058.0
33	36399.0
34	70081.0
35	234997.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.06187770084833	16.46144168752666	12.593115987298741	24.88356462432627
2	13.817108763314057	21.896546153597622	35.26477335142645	29.021571731661872
3	20.437275460709394	25.900311016532985	26.927914810762378	26.73449871199524
4	12.907373703651851	16.598856305932777	33.349343614374796	37.14442637604058
5	13.526390040600061	37.1567033180051	33.56882087510904	15.748085766285794
6	35.07709737380003	31.653754283486577	17.755334569648255	15.513813773065136
7	28.753028850813617	30.786047363149788	21.583940898370614	18.87698288766598
8	29.901246001916924	30.837955135315593	21.352832850512076	17.907966012255404
9	27.182657204088006	15.989963061481632	17.71476571502418	39.11261401940618
10	15.99836307440473	27.526411579094738	32.121003263081946	24.354222083418588
11	36.65636408671398	21.997049226229578	18.924152191003373	22.42243449605307
12	22.490926909118322	24.768191951064537	28.965613793251986	23.775267346565148
13	32.50869616722487	19.74541499294614	24.05612931712203	23.689759522706957
14	25.52290080446278	18.084581668587184	24.29520660801017	32.097310918939876
15	27.162411019093874	24.060437016056948	26.393702144157142	22.383449820692032
16	27.23241112678635	24.618284028129274	25.28446966841487	22.8648351766695
17	24.595453223774193	24.51640694831838	28.20185877209042	22.68628105581701
18	27.184380283661973	21.63627944042991	29.25767578103966	21.921664494868452
19	24.045144684837975	29.680261046555458	24.912284480437663	21.362309788168904
20	23.49505153084851	21.94277221964957	31.54614084021668	23.016035409285244
21	24.345175915655254	26.72345649762538	23.080650893309066	25.8507166934103
22	22.735819593568603	26.547702381080583	25.30557739319599	25.41090063215482
23	22.20511108478628	25.0143769451953	24.563360866709026	28.21715110330939
24	21.554863930559893	28.5701670310262	25.092346295917377	24.782622742496528
25	26.360748247304993	23.483636128670966	23.31692817988951	26.83868744413453
26	21.942987604596315	23.868313643559453	25.900901386002133	28.2877973658421
27	26.966195332608205	25.8367166718718	25.436746825764345	21.760341169755645
28	24.796838148981767	21.81224894192145	26.111117094026298	27.279795815070486
29	22.4071421648341	26.73508728474967	28.469582260895788	22.388188289520443
30	27.001518463874557	23.307666627179426	26.960810708939555	22.730004200006462
31	21.93221835725901	25.185177207964937	26.114563253174232	26.76804118160182
32	23.101543233143435	25.617239411137554	26.40253292697373	24.878684428745277
33	22.536157747934997	24.622591727064194	26.723887267518876	26.117363257481934
34	21.66298717382642	26.795179684891824	28.550351615925564	22.991481525356193
35	25.965947639919445	24.660284092744757	26.310132784819668	23.063635482516126
36	23.940682985666133	26.497302303542003	26.241424986807672	23.32058972398419
37	25.862132095587842	25.421023724651885	27.06893395220608	21.647910227554195
38	24.41496063840098	25.308808167397178	24.286591210140323	25.98963998406151
39	23.081943202989542	25.305146623302498	27.518442336065128	24.094467837642828
40	25.008776936579903	25.536039286214283	24.487329980507663	24.96785379669815
41	21.65049484691515	25.754008852321313	26.853979775353498	25.74151652541004
42	26.783548897767535	25.25108500166923	26.150317154334086	21.815048946229147
43	25.321300494308453	22.698557997781535	24.66502256157317	27.315118946336842
44	24.255791162755635	23.973852267465027	29.055644700991845	22.71471186878749
45	25.637916366025177	24.389545214684947	26.101855541316215	23.87068287797366
46	23.207562612538876	24.395843923116022	28.643028835798777	23.75356462854632
47	24.979699968769182	23.131697125687886	29.066198563382407	22.822404342160528
48	23.09185091053986	29.985246131147896	23.944344529760812	22.97855842855143
49	22.078464736099594	24.35314515868486	30.353769775030425	23.214620330185124
50	21.90938755290393	29.60272246572687	23.239174214114175	25.248715767255025
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	20.0
2	21.0
3	90.0
4	159.0
5	189.0
6	219.0
7	202.0
8	185.0
9	234.0
10	283.0
11	415.5
12	548.0
13	948.5
14	1349.0
15	1762.0
16	2175.0
17	2441.5
18	2708.0
19	2882.5
20	3057.0
21	3261.5
22	3466.0
23	3508.0
24	3550.0
25	3350.5
26	3151.0
27	3324.5
28	3498.0
29	3935.0
30	4372.0
31	4935.5
32	5499.0
33	6198.0
34	6897.0
35	7824.0
36	8751.0
37	10554.0
38	12357.0
39	14204.5
40	16052.0
41	18970.5
42	21889.0
43	26097.5
44	30306.0
45	37677.5
46	45049.0
47	50659.5
48	56270.0
49	51361.5
50	46453.0
51	39460.0
52	32467.0
53	29338.0
54	26209.0
55	26101.0
56	25993.0
57	25142.5
58	24292.0
59	22467.5
60	20643.0
61	18478.5
62	16314.0
63	14182.0
64	12050.0
65	10249.5
66	8449.0
67	7370.5
68	6292.0
69	5668.5
70	5045.0
71	3984.0
72	2923.0
73	2501.5
74	2080.0
75	1585.5
76	1091.0
77	944.5
78	798.0
79	677.0
80	556.0
81	459.5
82	363.0
83	287.0
84	211.0
85	177.5
86	144.0
87	99.0
88	54.0
89	34.5
90	15.0
91	12.5
92	10.0
93	5.0
94	0.0
95	1.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01701541079293968
2	0.0043076989349214384
3	2.153849467460719E-4
4	0.0
5	0.0
6	4.307698934921438E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.153849467460719E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	464285.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.40565310923846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.55996726753165	49.87088743472597
2	10.958583286363819	13.239207611295825
3	2.916148584004482	5.284555793411149
4	1.132223841440407	2.735708824322345
5	0.580230768894025	1.752460927455959
6	0.3561700056679071	1.2908809086174653
7	0.2545037805183844	1.07614269566883
8	0.19077617419026272	0.9219167519715727
9	0.1263452381185632	0.6868769963214655
>10	0.7859542759045478	9.21033200259876
>50	0.08066179104059759	3.3078373447812193
>100	0.052340539898771465	5.645953202556313
>500	0.0035849684862172236	1.5500818128221114
>1k	0.0025094779403520566	3.427157693451067
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	4639	0.9991707679550276	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	4016	0.8649859461322249	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1883	0.4055698547228534	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1847	0.3978159966399948	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1295	0.2789235060361631	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1076	0.2317542026987734	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1070	0.23046189301829692	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	994	0.21409263706559548	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	832	0.17920027569273184	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	824	0.17747719611876325	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	796	0.17144641760987325	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	656	0.14129252506542317	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	656	0.14129252506542317	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	644	0.1387079057044703	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	615	0.13246174224883422	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	594	0.12793865836716672	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA	547	0.11781556587010134	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	490	0.10553862390557524	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	473	0.10187707981089202	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.1538494674607192E-4	0.0
12	0.0	0.0	0.0	4.3076989349214383E-4	0.0
13	0.0	0.0	0.0	4.3076989349214383E-4	2.1538494674607192E-4
14	0.0	0.0	0.0	4.3076989349214383E-4	2.1538494674607192E-4
15	0.0	0.0	0.0	6.461548402382157E-4	4.3076989349214383E-4
16	0.0	0.0	0.0	8.615397869842877E-4	4.3076989349214383E-4
17	0.0	0.0	0.0	0.0010769247337303596	4.3076989349214383E-4
18	0.0	0.0	0.0	0.0019384645207146472	4.3076989349214383E-4
19	0.0	0.0	0.0	0.0023692344142067913	4.3076989349214383E-4
20	0.0	0.0	0.0	0.0034461591479371507	4.3076989349214383E-4
21	0.0	0.0	0.0	0.00560000861539787	4.3076989349214383E-4
22	0.0	0.0	0.0	0.0075384731361125175	6.461548402382157E-4
23	0.0	0.0	0.0	0.013784636591748603	6.461548402382157E-4
24	0.0	0.0	0.0	0.019815415100638615	6.461548402382157E-4
25	0.0	0.0	0.0	0.022615419408337552	6.461548402382157E-4
26	0.0	0.0	0.0	0.029292352757465782	8.615397869842877E-4
27	0.0	0.0	0.0	0.0368308258935783	8.615397869842877E-4
28	0.0	0.0	0.0	0.05384623668651798	8.615397869842877E-4
29	0.0	0.0	0.0	0.07301549694691838	8.615397869842877E-4
30	0.0	0.0	0.0	0.11092324757422704	8.615397869842877E-4
31	0.0	0.0	0.0	0.1680002584619361	8.615397869842877E-4
32	0.0	0.0	0.0	0.251569617799412	8.615397869842877E-4
33	0.0	0.0	0.0	0.3112312480480739	8.615397869842877E-4
34	0.0	0.0	0.0	0.4172006418471413	0.0010769247337303596
35	0.0	0.0	0.0	0.5108930936816826	0.0010769247337303596
36	0.0	0.0	0.0	0.6524010036938518	0.0010769247337303596
37	0.0	0.0	0.0	0.886524440806832	0.0010769247337303596
38	0.0	0.0	0.0	1.089632445588378	0.0012923096804764314
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCACTA	25	0.0023513618	35.19983	12
TGGTATA	330	0.0	25.333208	44
TCGTATA	105	1.10958354E-10	25.142733	44
GTACTCG	90	1.5075784E-8	24.444323	35
CGAACGT	55	1.5916102E-4	23.999884	26
CTTACAC	75	2.0598E-6	23.46655	3
GAACTAT	60	2.865005E-4	22.004633	1
ATTAGAG	120	5.9480953E-10	21.999893	3
CGCGCTA	50	0.00257814	21.999893	16
CATTCGA	60	2.8691828E-4	21.999893	22
TTAGTGT	60	2.8691828E-4	21.999893	4
TATAGAG	50	0.00257814	21.999893	2
ACGAAAA	85	6.1003375E-6	20.705782	23
GAACGTC	65	4.926036E-4	20.307592	27
ACCGACC	55	0.0044796513	19.999903	8
AGTATCC	55	0.0044796513	19.999903	8
TATTAGA	55	0.0044796513	19.999903	2
AACTATA	55	0.0044796513	19.999903	2
CTCGTAT	90	9.998255E-6	19.555458	43
ATGGGTG	285	0.0	19.298153	5
>>END_MODULE