##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062583_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7252
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.72214561500276	32.0	32.0	32.0	14.0	32.0
2	28.49200220628792	32.0	32.0	32.0	14.0	32.0
3	28.85314396028682	32.0	32.0	32.0	14.0	32.0
4	28.452289023717594	32.0	32.0	32.0	14.0	32.0
5	28.48607280750138	32.0	32.0	32.0	14.0	32.0
6	31.83370104798676	36.0	32.0	36.0	14.0	36.0
7	31.816740209597352	36.0	32.0	36.0	14.0	36.0
8	31.651682294539437	36.0	32.0	36.0	14.0	36.0
9	31.963320463320464	36.0	32.0	36.0	14.0	36.0
10	31.024131274131275	36.0	32.0	36.0	14.0	36.0
11	32.31508549365692	36.0	32.0	36.0	21.0	36.0
12	31.452289023717594	36.0	32.0	36.0	14.0	36.0
13	31.890788747931605	36.0	32.0	36.0	14.0	36.0
14	31.508825151682295	36.0	32.0	36.0	14.0	36.0
15	31.133066740209596	36.0	32.0	36.0	14.0	36.0
16	31.34542195256481	36.0	32.0	36.0	14.0	36.0
17	31.27302813017099	36.0	32.0	36.0	14.0	36.0
18	31.306536127964698	36.0	32.0	36.0	14.0	36.0
19	31.296607832322117	36.0	32.0	36.0	14.0	36.0
20	31.233590733590734	36.0	32.0	36.0	14.0	36.0
21	31.21704357418643	36.0	32.0	36.0	14.0	36.0
22	31.164506343077772	36.0	32.0	36.0	14.0	36.0
23	30.93188086045229	36.0	32.0	36.0	14.0	36.0
24	31.113899613899616	36.0	32.0	36.0	14.0	36.0
25	30.47200772200772	36.0	27.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	1.0
6	3.0
7	0.0
8	0.0
9	1.0
10	0.0
11	0.0
12	1.0
13	1.0
14	2.0
15	11.0
16	14.0
17	34.0
18	57.0
19	86.0
20	112.0
21	118.0
22	145.0
23	176.0
24	210.0
25	203.0
26	255.0
27	276.0
28	318.0
29	360.0
30	426.0
31	493.0
32	626.0
33	727.0
34	1313.0
35	1282.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.51241036955323	19.55322669608384	11.486486486486488	26.44787644787645
2	15.650854936569223	20.64258135686707	38.6238279095422	25.08273579702151
3	18.10535024820739	24.834528405956977	28.612796469939326	28.447324875896307
4	12.081092263136119	15.90125499931044	36.698386429458004	35.319266308095436
5	14.27783142502414	36.51538143192165	33.65981514691681	15.5469719961374
6	33.00220750551877	35.44426048565121	17.729028697571746	13.82450331125828
7	28.884902014904775	31.06541540160088	22.025945349158157	18.023737234336185
8	27.960253933204527	31.783052718741374	20.190449903394978	20.066243444659122
9	27.79848171152519	14.409937888198757	19.25465838509317	38.53692201518288
10	15.394173684937181	26.384094988264533	32.362280822863454	25.859450503934834
11	36.858089453340696	22.225289895085588	21.893981225842076	19.02263942573164
12	23.560281729042952	24.62367076370667	28.877226902361553	22.938820604888825
13	28.86925307193152	18.956233604859865	26.30125638547563	25.873256937732986
14	22.266703478741025	20.541137493097736	25.773053561568194	31.419105466593045
15	23.61954721148537	26.987852015461073	24.157923799006074	25.23467697404749
16	25.565985643291	25.828271673108777	24.751518498067366	23.854224185532853
17	24.09222697777164	26.13557917989783	25.99751484191633	23.774679000414192
18	23.34990334161834	24.71692902513118	26.981496824081745	24.95167080916874
19	25.248618784530386	25.345303867403313	26.712707182320443	22.693370165745858
20	25.32799337108134	24.803203977351195	25.79754177599779	24.07126087556967
21	26.294710675321088	24.678911752520367	25.54895732633614	23.4774202458224
22	24.87225521336832	24.89987570777517	25.728490539980665	24.499378538875845
23	23.795055931501174	25.217511393453947	25.90802375362519	25.079408921419695
24	24.043640381162824	25.452285595912166	26.41900290015191	24.085071122773098
25	24.672097197293937	24.65829076349579	27.06061024437388	23.60900179483639
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	4.0
3	8.0
4	8.0
5	8.0
6	11.0
7	14.0
8	14.0
9	14.0
10	19.0
11	24.0
12	24.0
13	24.0
14	29.5
15	35.0
16	35.0
17	35.0
18	53.5
19	72.0
20	72.0
21	72.0
22	109.0
23	146.0
24	146.0
25	146.0
26	208.0
27	270.0
28	270.0
29	270.0
30	321.5
31	373.0
32	373.0
33	373.0
34	457.0
35	541.0
36	541.0
37	541.0
38	585.0
39	629.0
40	629.0
41	629.0
42	695.5
43	762.0
44	762.0
45	762.0
46	824.5
47	887.0
48	887.0
49	887.0
50	900.0
51	913.0
52	913.0
53	913.0
54	840.5
55	768.0
56	768.0
57	768.0
58	691.5
59	615.0
60	615.0
61	615.0
62	537.0
63	459.0
64	459.0
65	459.0
66	398.0
67	337.0
68	337.0
69	337.0
70	258.0
71	179.0
72	179.0
73	179.0
74	143.5
75	108.0
76	108.0
77	108.0
78	77.5
79	47.0
80	47.0
81	47.0
82	43.0
83	39.0
84	39.0
85	39.0
86	25.5
87	12.0
88	12.0
89	12.0
90	8.5
91	5.0
92	5.0
93	5.0
94	3.0
95	1.0
96	1.0
97	1.0
98	4.5
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.013789299503585218
5	0.04136789851075565
6	0.05515719801434087
7	0.0827357970215113
8	0.0827357970215113
9	0.09652509652509653
10	0.12410369553226695
11	0.11031439602868175
12	0.1516822945394374
13	0.12410369553226695
14	0.11031439602868175
15	0.11031439602868175
16	0.11031439602868175
17	0.12410369553226695
18	0.13789299503585217
19	0.1654715940430226
20	0.1516822945394374
21	0.1516822945394374
22	0.1516822945394374
23	0.1516822945394374
24	0.1516822945394374
25	0.12410369553226695
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	7252.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.97517926089354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.80459770114943	85.30060672917816
2	3.0804597701149428	5.543298400441258
3	0.8888888888888888	2.399338113623828
4	0.47509578544061304	1.709873138444567
5	0.3218390804597701	1.4478764478764479
6	0.07662835249042146	0.4136789851075565
7	0.12260536398467432	0.7722007722007722
8	0.07662835249042146	0.5515719801434087
9	0.0	0.0
>10	0.15325670498084293	1.8615554329840043
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	31	0.4274682846111417	No Hit
TATCAACGCAGAGTACTTTTTTTTT	14	0.19305019305019305	No Hit
CTCCACTCCTGGTGGTGCCCTTCCG	13	0.17926089354660782	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	12	0.1654715940430226	No Hit
GGTATCAACGCAGAGTACTTTTTTT	12	0.1654715940430226	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	12	0.1654715940430226	No Hit
GTCCTATTCCATTATTCCTAGCTGC	11	0.1516822945394374	No Hit
GTATCTGATCGTCTTCGAACCTCCG	10	0.13789299503585217	No Hit
CTATTGGAGCTGGAATTACCGCGGC	10	0.13789299503585217	No Hit
GAACTACGACGGTATCTGATCGTCT	10	0.13789299503585217	No Hit
GAATAGGACCGCGGTTCTATTTTGT	8	0.11031439602868175	No Hit
GGCTCCACTCCTGGTGGTGCCCTTC	8	0.11031439602868175	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	8	0.11031439602868175	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	8	0.11031439602868175	No Hit
TTCGTATTGCGCCGCTAGAGGTGAA	8	0.11031439602868175	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE