##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062583_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7252
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.146580253723112	32.0	32.0	32.0	21.0	32.0
2	29.992553778268064	32.0	32.0	32.0	21.0	32.0
3	30.859487038058468	32.0	32.0	32.0	32.0	32.0
4	31.210976282404854	32.0	32.0	32.0	32.0	32.0
5	30.95146166574738	32.0	32.0	32.0	32.0	32.0
6	34.60852178709322	36.0	36.0	36.0	32.0	36.0
7	34.499586321014895	36.0	36.0	36.0	32.0	36.0
8	34.39975179260894	36.0	36.0	36.0	32.0	36.0
9	34.76447876447877	36.0	36.0	36.0	32.0	36.0
10	34.37989520132377	36.0	36.0	36.0	32.0	36.0
11	34.75372311086597	36.0	36.0	36.0	32.0	36.0
12	34.568532818532816	36.0	36.0	36.0	32.0	36.0
13	34.674020959735245	36.0	36.0	36.0	32.0	36.0
14	34.65802537231109	36.0	36.0	36.0	32.0	36.0
15	34.53130170987314	36.0	36.0	36.0	32.0	36.0
16	34.68629343629344	36.0	36.0	36.0	32.0	36.0
17	34.46594043022615	36.0	36.0	36.0	32.0	36.0
18	34.67650303364589	36.0	36.0	36.0	32.0	36.0
19	34.72007722007722	36.0	36.0	36.0	32.0	36.0
20	34.610314396028684	36.0	36.0	36.0	32.0	36.0
21	34.571704357418646	36.0	36.0	36.0	32.0	36.0
22	34.657060121345836	36.0	36.0	36.0	32.0	36.0
23	34.56136238279095	36.0	36.0	36.0	32.0	36.0
24	34.501378929950356	36.0	36.0	36.0	32.0	36.0
25	34.50965250965251	36.0	36.0	36.0	32.0	36.0
26	34.320739106453395	36.0	36.0	36.0	32.0	36.0
27	34.455046883618316	36.0	36.0	36.0	32.0	36.0
28	34.38279095421952	36.0	36.0	36.0	32.0	36.0
29	34.325289575289574	36.0	36.0	36.0	32.0	36.0
30	34.28792057363486	36.0	36.0	36.0	32.0	36.0
31	34.36196911196911	36.0	36.0	36.0	32.0	36.0
32	34.21511307225593	36.0	36.0	36.0	32.0	36.0
33	34.196911196911195	36.0	36.0	36.0	32.0	36.0
34	34.11900165471594	36.0	36.0	36.0	32.0	36.0
35	34.171263099834526	36.0	36.0	36.0	32.0	36.0
36	34.107832322118036	36.0	36.0	36.0	32.0	36.0
37	34.06094870380585	36.0	36.0	36.0	32.0	36.0
38	34.10838389409818	36.0	36.0	36.0	32.0	36.0
39	34.2373138444567	36.0	36.0	36.0	32.0	36.0
40	34.08287369001655	36.0	36.0	36.0	32.0	36.0
41	34.07790954219526	36.0	36.0	36.0	32.0	36.0
42	34.066740209597356	36.0	36.0	36.0	32.0	36.0
43	33.89327082184225	36.0	36.0	36.0	32.0	36.0
44	34.0015168229454	36.0	36.0	36.0	32.0	36.0
45	33.84280198565913	36.0	36.0	36.0	32.0	36.0
46	33.86362382790954	36.0	36.0	36.0	32.0	36.0
47	33.82446221731936	36.0	36.0	36.0	32.0	36.0
48	33.80722559293988	36.0	36.0	36.0	32.0	36.0
49	33.84969663541092	36.0	36.0	36.0	32.0	36.0
50	33.1676778819636	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0
22	0.0
23	1.0
24	21.0
25	32.0
26	36.0
27	60.0
28	93.0
29	176.0
30	246.0
31	369.0
32	588.0
33	913.0
34	1786.0
35	2929.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.80198565912852	18.80860452289024	11.982901268615555	26.406508549365693
2	18.080264791063303	19.997241759757276	36.46393600882637	25.45855744035305
3	20.90457804743519	23.276337562051847	28.530060672917816	27.289023717595146
4	14.106453392167678	16.009376723662438	36.95532266960838	32.9288472145615
5	17.20904578047435	34.03199117484832	32.34969663541092	16.409266409266408
6	33.57694429123001	37.16216216216216	15.347490347490348	13.913403199117486
7	29.74351902923332	30.8742415885273	20.435741864313293	18.94649751792609
8	26.696083838940982	32.722007722007724	19.47049089906233	21.11141753998897
9	26.847766133480423	13.582460011031438	18.270821842250413	41.29895201323773
10	15.88527302813017	26.199669056811913	31.97738554881412	25.937672366243795
11	38.27909542195256	20.36679536679537	21.897407611693325	19.45670159955874
12	24.089906232763376	23.44180915609487	28.971318257032543	23.49696635410921
13	29.757308328736897	19.277440706012133	24.88968560397132	26.075565361279647
14	23.538334252619965	19.897959183673468	24.68284611141754	31.880860452289024
15	26.489244346387203	26.158301158301157	21.48372862658577	25.868725868725868
16	25.39988968560397	26.585769442912298	23.538334252619965	24.47600661886376
17	23.593491450634307	26.309983452840598	25.11031439602868	24.986210700496414
18	23.483177054605626	26.29619415333701	25.758411472697183	24.462217319360178
19	24.324324324324326	26.75124103695532	25.02757859900717	23.89685603971318
20	25.44125758411473	24.958632101489243	24.71042471042471	24.88968560397132
21	25.992829564258134	25.510204081632654	24.351902923331494	24.145063430777718
22	25.28957528957529	25.89630446773304	24.227799227799228	24.58632101489244
23	23.745173745173744	25.28957528957529	26.33756205184777	24.627688913403198
24	24.351902923331494	25.910093767236624	25.81356867071153	23.924434638720353
25	24.269167126309984	25.3723110865968	24.931053502482072	25.427468284611145
26	24.145063430777718	26.44787644787645	25.179260893546605	24.227799227799228
27	25.63430777716492	25.386100386100384	24.531163816878102	24.448428019856593
28	23.64864864864865	26.585769442912298	25.303364589078875	24.462217319360178
29	24.13127413127413	24.848317705460563	25.44125758411473	25.57915057915058
30	24.903474903474905	24.724214009928296	26.254826254826252	24.117484831770547
31	24.31053502482074	25.785990071704358	25.537782680639825	24.36569222283508
32	24.87589630446773	25.303364589078875	23.93822393822394	25.882515168229453
33	23.428019856591284	25.261996690568118	26.172090457804742	25.137892995035855
34	24.903474903474905	25.951461665747377	24.47600661886376	24.669056811913954
35	24.87589630446773	26.075565361279647	25.013789299503586	24.034749034749034
36	24.07611693325979	25.02757859900717	25.39988968560397	25.49641478212907
37	24.269167126309984	26.33756205184777	24.958632101489243	24.434638720353004
38	24.669056811913954	25.661886376172088	24.57253171538886	25.096525096525095
39	24.531163816878102	25.57915057915058	25.137892995035855	24.751792608935467
40	25.34473248758963	26.04798676227248	23.93822393822394	24.669056811913954
41	24.269167126309984	26.806398234969663	25.23441809156095	23.690016547159402
42	25.854936569222282	25.799779371207944	24.641478212906783	23.70380584666299
43	24.462217319360178	25.565361279646993	25.89630446773304	24.07611693325979
44	24.87589630446773	25.427468284611145	24.351902923331494	25.34473248758963
45	23.772752344180915	26.489244346387203	24.87589630446773	24.862107004964148
46	24.462217319360178	25.8411472697187	24.57253171538886	25.12410369553227
47	24.42084942084942	25.510204081632654	24.806949806949806	25.261996690568118
48	24.324324324324326	26.116933259790404	24.36569222283508	25.193050193050194
49	24.034749034749034	25.77220077220077	24.641478212906783	25.551571980143407
50	24.751792608935467	25.16547159404302	24.627688913403198	25.45504688361831
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	0.5
8	0.0
9	0.5
10	1.0
11	2.5
12	4.0
13	5.0
14	6.0
15	8.5
16	11.0
17	13.0
18	15.0
19	17.0
20	19.0
21	24.5
22	30.0
23	43.5
24	57.0
25	55.5
26	54.0
27	83.5
28	113.0
29	124.5
30	136.0
31	123.5
32	111.0
33	161.0
34	211.0
35	218.5
36	226.0
37	263.0
38	300.0
39	323.5
40	347.0
41	377.0
42	407.0
43	415.5
44	424.0
45	439.5
46	455.0
47	515.5
48	576.0
49	556.0
50	536.0
51	462.0
52	388.0
53	410.0
54	432.0
55	447.0
56	462.0
57	438.0
58	414.0
59	378.0
60	342.0
61	311.0
62	280.0
63	247.5
64	215.0
65	187.0
66	159.0
67	141.0
68	123.0
69	115.5
70	108.0
71	92.5
72	77.0
73	69.0
74	61.0
75	47.5
76	34.0
77	37.5
78	41.0
79	29.5
80	18.0
81	15.0
82	12.0
83	10.5
84	9.0
85	7.0
86	5.0
87	4.5
88	4.0
89	3.0
90	2.0
91	2.5
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	11.0
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013789299503585218
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	7252.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.65747380033095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.44155258011133	87.47931605074463
2	2.692944185346773	4.936569222283508
3	0.7973521889574243	2.1924986210700497
4	0.5415977132540997	1.9856591285162712
5	0.22566571385587483	1.0341974627688912
6	0.060177523694899954	0.3309431880860452
7	0.09026628554234993	0.5791505791505791
8	0.030088761847449977	0.2206287920573635
9	0.045133142771174965	0.37231108659680084
>10	0.07522190461862494	0.8687258687258688
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	21	0.28957528957528955	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	12	0.1654715940430226	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	10	0.13789299503585217	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	10	0.13789299503585217	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	10	0.13789299503585217	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	9	0.12410369553226695	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG	9	0.12410369553226695	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG	9	0.12410369553226695	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC	8	0.11031439602868175	No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTT	8	0.11031439602868175	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.027578599007170437	0.0
33	0.0	0.0	0.0	0.04136789851075565	0.0
34	0.0	0.0	0.0	0.06894649751792609	0.0
35	0.0	0.0	0.0	0.09652509652509653	0.0
36	0.0	0.0	0.0	0.12410369553226697	0.0
37	0.0	0.0	0.0	0.1654715940430226	0.0
38	0.0	0.0	0.0	0.2344180915609487	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE