FastQCFastQC Report
Thu 2 Feb 2017
SRR4062582_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062582_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789263
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65100.8248201169952221No Hit
TATCAACGCAGAGTACTTTTTTTTT39060.49489207019713327No Hit
GTACTTTTTTTTTTTTTTTTTTTTT37320.47284618688574026No Hit
GAGTACTTTTTTTTTTTTTTTTTTT28350.3591958574011451No Hit
GTATTAGAGGCACTGCCTGCCCAGT25730.32600033195525446No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25280.32029881040920455No Hit
GTACATGGGGTGGTATCAACGCAAA24770.313837085990348No Hit
CCATAGGGTCTTCTCGTCTTATTAT22250.2819085653324684No Hit
GTCCTAAAGTGTGTATTTCTCATTT21280.2696186188887608No Hit
GGTATCAACGCAGAGTACTTTTTTT20590.2608762858514842No Hit
GTCCTACAGTGGACATTTCTAAATT20140.2551747643054343No Hit
CTCTAATACTTGTAATGCTAGAGGT19610.24845963892897555No Hit
CTGTAGGACGTGGAATATGGCAAGA18510.23452258626085348No Hit
CCATTGGGATGTCCTGATCCAACAT18030.22844096327840022No Hit
CTTTAGGACGTGAAATATGGCGAGG17190.21779812305910703No Hit
GTCAGGATACCGCGGCCGTTAAACT16660.21108299768264824No Hit
GTCCTACAGTGTGCATTTCTCATTT16230.20563487709420056No Hit
GATATACACTGTTCTACAAATCCCG15930.20183386273016726No Hit
CTTCTACACCATTGGGATGTCCTGA15820.20044015746335506No Hit
TTGTAGAACAGTGTATATCAATGAG15220.1928381287352885No Hit
GATATATTTTGATCAACGGACCAAG14870.18840361197724967No Hit
ATCCTGACCGTGCAAAGGTAGCATA14410.18257538995239864No Hit
GTTCATGCTAGTCCCTAATTAAGGA14370.1820685880371942No Hit
GGTCAGGATACCGCGGCCGTTAAAC14260.180674882770382No Hit
GTACATGGGTGGTATCAACGCAAAA14120.17890107606716646No Hit
CTGTTAGTATGAGTAACAAGAATTC13580.17205925021190654No Hit
GTATCAACGCAGAGTACATGGGGTG13510.17117234686029878No Hit
GCCTAAAGGAAAGATCCAAAAAGAT13370.16939854015708328No Hit
CCTCTAGCATTACAAGTATTAGAGG13280.16825823584787328No Hit
GTATCCTGACCGTGCAAAGGTAGCA13200.1672446320174644No Hit
GTGTATATCAATGAGTTACAATGAA12710.16103630855621004No Hit
ATCTATAACTTTATAGATGCAACAC12520.15862899945898895No Hit
GTACATGGGAGAAATCGTAAATAGA12110.15343427982814345No Hit
GCAGAGTACTTTTTTTTTTTTTTTT12060.15280077743413792No Hit
GTTATATAATTTAAGCTCCATAGGG12020.1522939755189335No Hit
TGCTAGAGGTGATGTTTTTGGTAAA11980.15178717360372904No Hit
ATCGTAAATAGATAGAAACCGACCT11950.1514070721673257No Hit
GTTATAGATTAACCCAATTTTAAGT11920.1510269707309224No Hit
CTGTAGGACCTGGAATATGGCGAGA11880.15052016881571795No Hit
ACCTATAACTTCTCTGTTAACCCAA11820.1497599659429113No Hit
GTATCAACGCAGAGTACATGGGAGA11500.1457055506212758No Hit
CACTATAAATAATCCACCTATAACT11470.14532544918487247No Hit
GTGTATATCAATGAGTTACAATGAG11110.14076423194803253No Hit
GTGTAGAAGCTATTAATGGTTCGTT10980.1391171257236181No Hit
CTATAGAACTAGTACCGCAAGGGAA10870.1377234204568059No Hit
CCTATAACTTCTCTGTTAACCCAAC10720.13582291327478926No Hit
GATTAAAGATAAGAGACAGTTGGAC10710.13569621279598815No Hit
AAATATATCTGGGTCAATAAGATAT10680.13531611135958482No Hit
ATAAATAATCCACCTATAACTTCTC10530.13341560417756818No Hit
AGCATGAACGGCTAAACGAGGGTCC10450.13240200034715932No Hit
GACTATAGGCAATAATCACACTATA10360.13126169603794932No Hit
TCCCAATGGTGTAGAAGCTATTAAT10340.13100829508034711No Hit
GTCCTGATCCAACATCGAGGTCGTA10180.12898108741952935No Hit
GACCTGGATTGCTCCGGTCTGAACT10170.12885438694072826No Hit
CTGTTAACCCAACACCGGAATGCCT10090.12784078311031938No Hit
GATTAAACCTTGTACCTTTTGCATA9980.12644707784350717No Hit
GTTCTACAGTGTGGTTTTTATCATT9900.1254334740130983No Hit
GATAGAAACCGACCTGGATTGCTCC9720.12315286539467832No Hit
GGATTGCTCCGGTCTGAACTCAGAT9220.11681784145462284No Hit
GGATACCGCGGCCGTTAAACTTTAG8970.11365032948459511No Hit
GTTAGTATGAGTAACAAGAATTCCA8860.11225662421778292No Hit
GATATACACTGTTCTACAATGCCGG8850.11212992373898181No Hit
TACTAACAGTGTTGCATCTATAAAG8700.11022941655696517No Hit
ACCTTTGCACGGTCAGGATACCGCG8680.10997601559936294No Hit
AGGGATAACAGCGCAATCCTATTTA8460.10718860506573853No Hit
ATCAACGCAGAGTACTTTTTTTTTT8440.10693520410813634No Hit
GTACATGGGAGTGGTATCAACGCAA8180.10364099165930747No Hit
GGGCAGGCAGTGCCTCTAATACTTG8110.1027540883076997No Hit
GTTATACGCGTATGCCTGGAGAATT8110.1027540883076997No Hit
ACCCTATGGAGCTTAAATTATATAA7970.10098028160448419No Hit
ATTTAGAAATGTCCACTGTAGGACG7930.10047347968927973No Hit
CTTAAATAGGATTGCGCTGTTATCC7900.10009337825287641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACCC451.663986E-619.0027312
CACCTTT2500.015.20121914
CCACCTT2400.015.04287213
AACTTGC953.0377123E-1015.0021537
CCAACGA1151.8189894E-1214.87358919
AAACTTG1058.731149E-1114.478276
TGTCCAC2700.014.42799910
ATGTCCA2900.014.088239
CTTTTTC2800.013.91447817
AAATGTC2800.013.9127127
AATGTCC2950.013.8494478
CTCTAGG551.9716908E-413.8070221
TAGGACC4700.013.7431664
TAGAAAT2900.013.4295544
GTCCACT2700.013.37059411
GAAATGT2850.013.3352486
ACTCGGC1301.4551915E-1113.15406611
ATAGGAC802.008299E-613.0552623
TGAACTT957.35472E-813.00186610
ATTTAGA2850.012.9895021
Produced by FastQC (version 0.11.5)