##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062582_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	789263
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.939686771076307	32.0	32.0	32.0	32.0	32.0
2	30.673273674301214	32.0	32.0	32.0	32.0	32.0
3	30.72933103414198	32.0	32.0	32.0	32.0	32.0
4	30.737807296173774	32.0	32.0	32.0	32.0	32.0
5	30.629857221230438	32.0	32.0	32.0	32.0	32.0
6	34.20556392482607	36.0	36.0	36.0	32.0	36.0
7	33.97073979142567	36.0	36.0	36.0	32.0	36.0
8	33.94207760911129	36.0	36.0	36.0	32.0	36.0
9	34.06550794855454	36.0	36.0	36.0	32.0	36.0
10	33.814111392526954	36.0	36.0	36.0	32.0	36.0
11	34.22779225682694	36.0	36.0	36.0	32.0	36.0
12	33.98469838317519	36.0	36.0	36.0	32.0	36.0
13	34.13013279477183	36.0	36.0	36.0	32.0	36.0
14	34.028342897107805	36.0	36.0	36.0	32.0	36.0
15	34.00778447741754	36.0	36.0	36.0	32.0	36.0
16	33.980808678475995	36.0	36.0	36.0	32.0	36.0
17	33.92705726734941	36.0	36.0	36.0	32.0	36.0
18	34.01454774897594	36.0	36.0	36.0	32.0	36.0
19	33.920076070967475	36.0	36.0	36.0	32.0	36.0
20	33.84658979326283	36.0	36.0	36.0	32.0	36.0
21	33.81218554524918	36.0	36.0	36.0	32.0	36.0
22	33.78559871677755	36.0	36.0	36.0	32.0	36.0
23	33.830381001009805	36.0	36.0	36.0	32.0	36.0
24	33.818383732672125	36.0	36.0	36.0	32.0	36.0
25	33.43286838480962	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	30.0
5	65.0
6	152.0
7	29.0
8	108.0
9	136.0
10	96.0
11	33.0
12	54.0
13	36.0
14	389.0
15	418.0
16	519.0
17	700.0
18	861.0
19	1124.0
20	1693.0
21	2320.0
22	3321.0
23	4551.0
24	5890.0
25	7965.0
26	10622.0
27	13229.0
28	17110.0
29	22338.0
30	28036.0
31	37458.0
32	53391.0
33	76506.0
34	171193.0
35	328887.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.58712053056663	21.863825624624617	12.387828594743498	23.161225250065257
2	13.50857542522351	22.572576884870355	43.608850122650885	20.309997567255255
3	17.98637904273179	28.455383445785422	31.386993569641103	22.17124394184168
4	9.65350235822159	18.39248106199804	42.334069142398064	29.61994743738231
5	9.998060794719278	41.577448436785076	36.847942224357176	11.576548544138467
6	26.136805852206685	42.5962005275707	19.797211049830207	11.469782570392407
7	24.8055430531167	35.50736305603276	22.729560625558648	16.95753326529189
8	23.61278786649082	38.00243481789592	21.32050826823577	17.064269047377497
9	26.603535978024738	15.711019209331878	22.5955362113724	35.089908601270984
10	14.5530169264491	28.703041281052116	35.47488938951128	21.269052402987498
11	31.232146209541977	24.868712801960555	27.64324892052748	16.25589206796998
12	23.070199253528738	28.283192217618446	32.26153896899683	16.38506955985598
13	26.46399967527278	23.547035976223878	29.316144777599913	20.672819570903428
14	19.618177782006153	25.84695398471443	29.241239336568043	25.293628896711382
15	21.797366304835883	33.060839829629195	26.355597046161964	18.786196819372954
16	19.86953621048733	30.366759764490407	30.254763336199836	19.508940688822427
17	17.483851653397874	30.40785674780164	30.712556893428523	21.395734705371957
18	17.58667495464867	29.551180403151122	34.51889533039871	18.343249311801493
19	21.48618476137315	28.226600360287215	30.43627737041078	19.850937507928855
20	20.685779668133485	30.406721051743624	31.164145933994565	17.74335334612833
21	22.071444066265713	27.367905607264415	29.101931925290604	21.458718401179276
22	20.347056390319167	31.005920463283232	29.411861735738736	19.23516141065886
23	20.459213903546555	30.503836086747455	30.80047147009603	18.23647853960996
24	20.656932732331185	30.353991619293136	30.728497321243935	18.260578327131746
25	20.100904128392067	30.511130027287503	31.906585662581367	17.481380181739066
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	562.0
1	562.0
2	1024.0
3	1486.0
4	1486.0
5	1486.0
6	4137.0
7	6788.0
8	6788.0
9	6788.0
10	6712.0
11	6636.0
12	6636.0
13	6636.0
14	6746.0
15	6856.0
16	6856.0
17	6856.0
18	10943.5
19	15031.0
20	15031.0
21	15031.0
22	23673.0
23	32315.0
24	32315.0
25	32315.0
26	48889.5
27	65464.0
28	65464.0
29	65464.0
30	80891.0
31	96318.0
32	96318.0
33	96318.0
34	99239.0
35	102160.0
36	102160.0
37	102160.0
38	97482.0
39	92804.0
40	92804.0
41	92804.0
42	87413.5
43	82023.0
44	82023.0
45	82023.0
46	90164.0
47	98305.0
48	98305.0
49	98305.0
50	86627.5
51	74950.0
52	74950.0
53	74950.0
54	59314.5
55	43679.0
56	43679.0
57	43679.0
58	34428.0
59	25177.0
60	25177.0
61	25177.0
62	20183.0
63	15189.0
64	15189.0
65	15189.0
66	12831.0
67	10473.0
68	10473.0
69	10473.0
70	8404.5
71	6336.0
72	6336.0
73	6336.0
74	5029.5
75	3723.0
76	3723.0
77	3723.0
78	2711.0
79	1699.0
80	1699.0
81	1699.0
82	1184.5
83	670.0
84	670.0
85	670.0
86	446.5
87	223.0
88	223.0
89	223.0
90	148.5
91	74.0
92	74.0
93	74.0
94	56.5
95	39.0
96	39.0
97	39.0
98	161.0
99	283.0
100	283.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008742333037276548
2	0.003927714842834391
3	0.0048146181944421565
4	0.013810352189320925
5	0.035476134064310626
6	0.046118974283603814
7	0.06689785280698576
8	0.08907043659717989
9	0.09375835431282095
10	0.11542413618781065
11	0.11593093810301508
12	0.12936118885593267
13	0.11517073523020843
14	0.11884504911544062
15	0.10959591416295962
16	0.10706190458693743
17	0.11998535342465058
18	0.12327956587347945
19	0.1270805802375127
20	0.11833824720023617
21	0.12441987018268943
22	0.1455788501424747
23	0.13810352189320924
24	0.12974129029233603
25	0.1254334740130983
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	789263.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.496863070911527
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.4110302781267	21.064013818666204
2	14.67933439725103	8.659886333756704
3	5.2722533795809	4.665448080379451
4	2.432366963800121	2.8698878107768397
5	1.3261997711261515	1.955936652679115
6	0.8213362416701744	1.453610559343334
7	0.5664331445462838	1.1695600632455303
8	0.4115728118125556	0.9712085498995995
9	0.30919055565753345	0.8208136334744383
>10	2.1087780159409943	12.176107218689863
>50	0.28652247130922415	5.948644022624725
>100	0.31457060475372983	19.402334391935323
>500	0.036678328400640656	7.346835022152895
>1k	0.023301526278054062	10.667109071249072
>5k	4.3150974588989006E-4	0.8286047711268923
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6510	0.8248201169952221	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3906	0.49489207019713327	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3732	0.47284618688574026	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2835	0.3591958574011451	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	2573	0.32600033195525446	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2528	0.32029881040920455	No Hit
GTACATGGGGTGGTATCAACGCAAA	2477	0.313837085990348	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	2225	0.2819085653324684	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2128	0.2696186188887608	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2059	0.2608762858514842	No Hit
GTCCTACAGTGGACATTTCTAAATT	2014	0.2551747643054343	No Hit
CTCTAATACTTGTAATGCTAGAGGT	1961	0.24845963892897555	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1851	0.23452258626085348	No Hit
CCATTGGGATGTCCTGATCCAACAT	1803	0.22844096327840022	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1719	0.21779812305910703	No Hit
GTCAGGATACCGCGGCCGTTAAACT	1666	0.21108299768264824	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1623	0.20563487709420056	No Hit
GATATACACTGTTCTACAAATCCCG	1593	0.20183386273016726	No Hit
CTTCTACACCATTGGGATGTCCTGA	1582	0.20044015746335506	No Hit
TTGTAGAACAGTGTATATCAATGAG	1522	0.1928381287352885	No Hit
GATATATTTTGATCAACGGACCAAG	1487	0.18840361197724967	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	1441	0.18257538995239864	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	1437	0.1820685880371942	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	1426	0.180674882770382	No Hit
GTACATGGGTGGTATCAACGCAAAA	1412	0.17890107606716646	No Hit
CTGTTAGTATGAGTAACAAGAATTC	1358	0.17205925021190654	No Hit
GTATCAACGCAGAGTACATGGGGTG	1351	0.17117234686029878	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	1337	0.16939854015708328	No Hit
CCTCTAGCATTACAAGTATTAGAGG	1328	0.16825823584787328	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	1320	0.1672446320174644	No Hit
GTGTATATCAATGAGTTACAATGAA	1271	0.16103630855621004	No Hit
ATCTATAACTTTATAGATGCAACAC	1252	0.15862899945898895	No Hit
GTACATGGGAGAAATCGTAAATAGA	1211	0.15343427982814345	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1206	0.15280077743413792	No Hit
GTTATATAATTTAAGCTCCATAGGG	1202	0.1522939755189335	No Hit
TGCTAGAGGTGATGTTTTTGGTAAA	1198	0.15178717360372904	No Hit
ATCGTAAATAGATAGAAACCGACCT	1195	0.1514070721673257	No Hit
GTTATAGATTAACCCAATTTTAAGT	1192	0.1510269707309224	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1188	0.15052016881571795	No Hit
ACCTATAACTTCTCTGTTAACCCAA	1182	0.1497599659429113	No Hit
GTATCAACGCAGAGTACATGGGAGA	1150	0.1457055506212758	No Hit
CACTATAAATAATCCACCTATAACT	1147	0.14532544918487247	No Hit
GTGTATATCAATGAGTTACAATGAG	1111	0.14076423194803253	No Hit
GTGTAGAAGCTATTAATGGTTCGTT	1098	0.1391171257236181	No Hit
CTATAGAACTAGTACCGCAAGGGAA	1087	0.1377234204568059	No Hit
CCTATAACTTCTCTGTTAACCCAAC	1072	0.13582291327478926	No Hit
GATTAAAGATAAGAGACAGTTGGAC	1071	0.13569621279598815	No Hit
AAATATATCTGGGTCAATAAGATAT	1068	0.13531611135958482	No Hit
ATAAATAATCCACCTATAACTTCTC	1053	0.13341560417756818	No Hit
AGCATGAACGGCTAAACGAGGGTCC	1045	0.13240200034715932	No Hit
GACTATAGGCAATAATCACACTATA	1036	0.13126169603794932	No Hit
TCCCAATGGTGTAGAAGCTATTAAT	1034	0.13100829508034711	No Hit
GTCCTGATCCAACATCGAGGTCGTA	1018	0.12898108741952935	No Hit
GACCTGGATTGCTCCGGTCTGAACT	1017	0.12885438694072826	No Hit
CTGTTAACCCAACACCGGAATGCCT	1009	0.12784078311031938	No Hit
GATTAAACCTTGTACCTTTTGCATA	998	0.12644707784350717	No Hit
GTTCTACAGTGTGGTTTTTATCATT	990	0.1254334740130983	No Hit
GATAGAAACCGACCTGGATTGCTCC	972	0.12315286539467832	No Hit
GGATTGCTCCGGTCTGAACTCAGAT	922	0.11681784145462284	No Hit
GGATACCGCGGCCGTTAAACTTTAG	897	0.11365032948459511	No Hit
GTTAGTATGAGTAACAAGAATTCCA	886	0.11225662421778292	No Hit
GATATACACTGTTCTACAATGCCGG	885	0.11212992373898181	No Hit
TACTAACAGTGTTGCATCTATAAAG	870	0.11022941655696517	No Hit
ACCTTTGCACGGTCAGGATACCGCG	868	0.10997601559936294	No Hit
AGGGATAACAGCGCAATCCTATTTA	846	0.10718860506573853	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	844	0.10693520410813634	No Hit
GTACATGGGAGTGGTATCAACGCAA	818	0.10364099165930747	No Hit
GGGCAGGCAGTGCCTCTAATACTTG	811	0.1027540883076997	No Hit
GTTATACGCGTATGCCTGGAGAATT	811	0.1027540883076997	No Hit
ACCCTATGGAGCTTAAATTATATAA	797	0.10098028160448419	No Hit
ATTTAGAAATGTCCACTGTAGGACG	793	0.10047347968927973	No Hit
CTTAAATAGGATTGCGCTGTTATCC	790	0.10009337825287641	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.267004788011094E-4	0.0
4	0.0	0.0	0.0	1.267004788011094E-4	0.0
5	0.0	0.0	0.0	1.267004788011094E-4	0.0
6	0.0	0.0	0.0	1.267004788011094E-4	0.0
7	0.0	0.0	0.0	2.534009576022188E-4	0.0
8	0.0	0.0	0.0	3.801014364033282E-4	0.0
9	0.0	0.0	0.0	5.068019152044376E-4	0.0
10	0.0	0.0	0.0	5.068019152044376E-4	0.0
11	0.0	0.0	1.267004788011094E-4	5.068019152044376E-4	0.0
12	0.0	0.0	2.534009576022188E-4	5.068019152044376E-4	0.0
13	0.0	0.0	2.534009576022188E-4	5.068019152044376E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTACCC	45	1.663986E-6	19.00273	12
CACCTTT	250	0.0	15.201219	14
CCACCTT	240	0.0	15.042872	13
AACTTGC	95	3.0377123E-10	15.002153	7
CCAACGA	115	1.8189894E-12	14.873589	19
AAACTTG	105	8.731149E-11	14.47827	6
TGTCCAC	270	0.0	14.427999	10
ATGTCCA	290	0.0	14.08823	9
CTTTTTC	280	0.0	13.914478	17
AAATGTC	280	0.0	13.912712	7
AATGTCC	295	0.0	13.849447	8
CTCTAGG	55	1.9716908E-4	13.807022	1
TAGGACC	470	0.0	13.743166	4
TAGAAAT	290	0.0	13.429554	4
GTCCACT	270	0.0	13.370594	11
GAAATGT	285	0.0	13.335248	6
ACTCGGC	130	1.4551915E-11	13.154066	11
ATAGGAC	80	2.008299E-6	13.055262	3
TGAACTT	95	7.35472E-8	13.001866	10
ATTTAGA	285	0.0	12.989502	1
>>END_MODULE