FastQCFastQC Report
Thu 2 Feb 2017
SRR4062581_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062581_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093697
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG40840.37341238021133827No Hit
TCCATGTACTCTGCGTTGATACCAC36040.32952453924624464No Hit
GAGTACATGGAAGCAGTGGTATCAA17120.15653329944216726No Hit
CATGTACTCTGCGTTGATACCACTG17040.15580183542608236No Hit
GTATCAACGCAGAGTACTTTTTTTT12690.11602847955146628No Hit
CCCATGTACTCTGCGTTGATACCAC12270.11218829346702057No Hit
GCTTCCATGTACTCTGCGTTGATAC12180.11136539644892507No Hit
GTACATGGGAAGCAGTGGTATCAAC11080.10130776622775779No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT400.005277349614.2501737
ACGCGTA400.005277349614.2501736
TACGCGT400.005282161814.2482155
CTTACTC602.5721358E-514.2469093
GTCTTAA707.3016654E-613.5635171
ATACCGT1850.013.3515126
GTCCCTA500.001498912813.3001611
ACCGTCG1600.013.0626588
CGCATCG1102.741217E-912.95470113
CGACCAT1102.741217E-912.95470110
CAAGACG2350.012.9345494
ACTGCGC604.0898772E-412.6668218
GTAGTAC604.0977844E-412.6639193
AAGACGG2300.012.3897525
CGCAAGA2150.012.3671462
GTATTAC1001.4518628E-712.3428011
TACCGTC1700.012.2942667
CTTATAC701.09253786E-412.2116373
CGTCGTA1800.012.13903510
GGGCTAG550.003080866812.0838611
Produced by FastQC (version 0.11.5)