FastQCFastQC Report
Thu 2 Feb 2017
SRR4062581_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062581_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093697
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30030.274573305037867No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC23880.2183420088013408No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA11280.10313642626797001No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC11270.1030449932659594No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAT602.8726767E-421.999843
CCGATCA755.4723318E-520.5331849
ATCACGC654.931993E-420.3075433
GTGTACG708.108074E-418.862181
GCACCGT708.1230444E-418.8570066
GTAGTAC708.1230444E-418.8570063
CCGTCGT2000.018.6998659
ACCGTCG1901.8189894E-1218.5261828
TACCGTC1901.8189894E-1218.5261827
GGTATAC600.00740025318.338231
TATGCGT600.007411972618.333239
TTAGGAC1801.4551915E-1118.3331993
ATACCGT2300.018.173786
GTATAGG1002.4769706E-517.60471
CGTCGTA2150.017.39522210
ACGATTT902.2152522E-417.11098531
GGTATAT1206.3891694E-616.5044081
TATAGGG800.001987676416.5006352
ACGATTA800.001988283816.4998832
TAGGACG1351.0852964E-616.2961774
Produced by FastQC (version 0.11.5)