##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062581_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1093697
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.308603754056197	32.0	32.0	32.0	32.0	32.0
2	31.395642485990177	32.0	32.0	32.0	32.0	32.0
3	31.490667890649785	32.0	32.0	32.0	32.0	32.0
4	31.59342121263933	32.0	32.0	32.0	32.0	32.0
5	31.528820139398753	32.0	32.0	32.0	32.0	32.0
6	35.11900828108699	36.0	36.0	36.0	36.0	36.0
7	35.13431690861363	36.0	36.0	36.0	36.0	36.0
8	35.07743369507277	36.0	36.0	36.0	36.0	36.0
9	35.2155249580094	36.0	36.0	36.0	36.0	36.0
10	35.08605857015243	36.0	36.0	36.0	36.0	36.0
11	35.21388099263324	36.0	36.0	36.0	36.0	36.0
12	35.13950847446779	36.0	36.0	36.0	36.0	36.0
13	35.17965396266059	36.0	36.0	36.0	36.0	36.0
14	35.14009456001068	36.0	36.0	36.0	36.0	36.0
15	35.100155710402426	36.0	36.0	36.0	36.0	36.0
16	35.11918748977093	36.0	36.0	36.0	36.0	36.0
17	35.085414881818274	36.0	36.0	36.0	36.0	36.0
18	35.09408638772896	36.0	36.0	36.0	36.0	36.0
19	35.084630386661026	36.0	36.0	36.0	36.0	36.0
20	35.08401047090739	36.0	36.0	36.0	36.0	36.0
21	35.07402781574787	36.0	36.0	36.0	36.0	36.0
22	35.05549891789042	36.0	36.0	36.0	36.0	36.0
23	35.008387149274434	36.0	36.0	36.0	36.0	36.0
24	34.99305932081737	36.0	36.0	36.0	36.0	36.0
25	34.96600886717253	36.0	36.0	36.0	32.0	36.0
26	34.916260170778564	36.0	36.0	36.0	32.0	36.0
27	34.90929023303529	36.0	36.0	36.0	32.0	36.0
28	34.89484199005758	36.0	36.0	36.0	32.0	36.0
29	34.87035531778911	36.0	36.0	36.0	32.0	36.0
30	34.849407102698464	36.0	36.0	36.0	32.0	36.0
31	34.84136465584161	36.0	36.0	36.0	32.0	36.0
32	34.81460770213322	36.0	36.0	36.0	32.0	36.0
33	34.796258927289735	36.0	36.0	36.0	32.0	36.0
34	34.77952211627169	36.0	36.0	36.0	32.0	36.0
35	34.755757764719114	36.0	36.0	36.0	32.0	36.0
36	34.71856007651114	36.0	36.0	36.0	32.0	36.0
37	34.71008698021481	36.0	36.0	36.0	32.0	36.0
38	34.6622876354237	36.0	36.0	36.0	32.0	36.0
39	34.64148571313627	36.0	36.0	36.0	32.0	36.0
40	34.601264335551804	36.0	36.0	36.0	32.0	36.0
41	34.58918786464624	36.0	36.0	36.0	32.0	36.0
42	34.53411502454519	36.0	36.0	36.0	32.0	36.0
43	34.523655089115174	36.0	36.0	36.0	32.0	36.0
44	34.518752451547364	36.0	36.0	36.0	32.0	36.0
45	34.44727744521563	36.0	36.0	36.0	32.0	36.0
46	34.46798976316109	36.0	36.0	36.0	32.0	36.0
47	34.4387312025177	36.0	36.0	36.0	32.0	36.0
48	34.404245417149355	36.0	36.0	36.0	32.0	36.0
49	34.412510960531115	36.0	36.0	36.0	32.0	36.0
50	33.867810737343156	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	7.0
21	16.0
22	71.0
23	255.0
24	664.0
25	1531.0
26	3303.0
27	6202.0
28	10507.0
29	16399.0
30	24435.0
31	35468.0
32	53152.0
33	87557.0
34	190613.0
35	663515.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.73842557876678	17.40808682324797	11.218327401484364	26.63516019650088
2	16.184613809095364	20.287823593524838	36.15679884716949	27.3707637502103
3	18.774167062117293	23.8086878445335	28.611332837762838	28.805812255586368
4	12.515452191468196	15.543624553806543	35.667132365849376	36.27379088887589
5	14.236118413052242	36.68666915973986	33.76895063257923	15.308261794628677
6	35.02664824589716	34.901841741695335	16.466595287708707	13.604914724698796
7	30.124733015390014	30.34069796360232	20.99687664579554	18.537692375212124
8	28.29698563403359	33.01026976417579	19.56841754930072	19.124327052489907
9	27.027778260340845	14.213351595551602	18.350603503529772	40.408266640577786
10	15.362755863827001	27.062431368102867	32.24924270616085	25.325570061909286
11	37.79648293814466	20.946477863613048	21.727407133785682	19.52963206445661
12	24.12432328149387	23.62537338952196	29.047258975749223	23.203044353234944
13	29.741601193017807	19.25176717134636	25.134932252717164	25.871699382918667
14	23.32775896797742	19.406837542756357	25.10969674416223	32.15570674510399
15	25.349342642432042	26.91558996687382	22.519674096207634	25.2153932944865
16	26.0701089972817	25.503681549825956	23.81189671362361	24.614312739268737
17	24.210910334397916	25.895654829445448	25.43931271641049	24.454122119746145
18	25.35025697245215	24.36177478771543	26.43136078822562	23.8566074516068
19	25.337182053164632	25.806964817495153	25.055019808959884	23.800833320380324
20	25.220604975601102	24.39194767837893	25.697062349078404	24.690384996941567
21	26.07742363744255	24.818756931764465	24.259826990473595	24.843992440319393
22	25.074495038388143	24.85103278147421	24.88961750832269	25.184854671814954
23	24.190612207951563	24.396062163469406	25.226730986735813	26.186594641843215
24	24.491975382578538	25.55652982498809	24.970444282100072	24.9810505103333
25	25.088575720697783	24.50258161081177	24.8431695433013	25.565673125189154
26	24.527177088352627	25.05236825190158	25.409414124753017	25.01104053499278
27	25.49664120867114	25.082449709563072	24.676761479641986	24.744147602123807
28	24.665149488386636	24.334984918126317	25.578473745470635	25.421391848016405
29	24.495998434667005	25.147641439996637	25.604440718041648	24.751919407294707
30	25.038744734602	24.90799554172682	25.624007380471923	24.42925234319926
31	24.740490282043382	24.90168666458809	24.768834512666672	25.588988540701855
32	24.59840339691889	24.945574505553182	24.966695529017635	25.48932656851029
33	24.37238101594866	24.53741758457781	25.404751041650474	25.685450357823054
34	24.726592465737767	24.985073562421768	25.758688192433553	24.529645779406913
35	25.71333742343629	24.68480758381892	25.26202412551191	24.33983086723288
36	24.775509121813446	25.1972895600884	25.034081651499456	24.9931196665987
37	25.735281343918835	24.840883718251032	24.963129641939222	24.460705295890907
38	24.732718476872478	24.87462249599295	25.18595186783908	25.206707159295487
39	25.023658289270244	24.863376236745644	24.905618283674546	25.207347190309566
40	25.471954298128274	24.953529176728107	25.014240690063154	24.560275835080468
41	24.246934937190098	25.105673692073765	25.674021232571725	24.97337013816441
42	26.01195760800295	25.204695633251255	24.77148606972498	24.011860689020818
43	25.15961916326003	24.08857297770772	25.147641439996637	25.604166419035618
44	24.97830752027298	24.672281262543468	25.394053380415233	24.955357836768318
45	25.068003295245394	24.982147706357427	25.105490826069744	24.844358172327436
46	24.653742904792555	24.680715665047693	25.609127216337995	25.05641421382176
47	25.098999082926987	24.45823660483662	25.69541655504221	24.747347757194177
48	25.27875636487985	25.809799240557485	24.566218980211154	24.345225414351507
49	24.46399688396329	25.08363833858921	25.48429775339971	24.968067024047794
50	24.526171325330505	25.86392757774777	24.53229733646522	25.07760376045651
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10.0
1	12.0
2	14.0
3	81.0
4	148.0
5	158.0
6	168.0
7	149.5
8	131.0
9	188.5
10	246.0
11	374.0
12	502.0
13	826.5
14	1151.0
15	1524.0
16	1897.0
17	2113.0
18	2329.0
19	2523.0
20	2717.0
21	3296.0
22	3875.0
23	4351.5
24	4828.0
25	5912.0
26	6996.0
27	8679.5
28	10363.0
29	11912.5
30	13462.0
31	14959.5
32	16457.0
33	18743.0
34	21029.0
35	24434.0
36	27839.0
37	33106.0
38	38373.0
39	42040.0
40	45707.0
41	50724.0
42	55741.0
43	57614.5
44	59488.0
45	67852.0
46	76216.0
47	83459.5
48	90703.0
49	90850.5
50	90998.0
51	86049.0
52	81100.0
53	79222.5
54	77345.0
55	78144.5
56	78944.0
57	76259.5
58	73575.0
59	67215.5
60	60856.0
61	53431.0
62	46006.0
63	39455.5
64	32905.0
65	27863.0
66	22821.0
67	19383.0
68	15945.0
69	14463.5
70	12982.0
71	9916.5
72	6851.0
73	6169.0
74	5487.0
75	3928.5
76	2370.0
77	2117.5
78	1865.0
79	1619.0
80	1373.0
81	1068.5
82	764.0
83	664.0
84	564.0
85	456.5
86	349.0
87	254.0
88	159.0
89	91.5
90	24.0
91	19.0
92	14.0
93	9.5
94	5.0
95	4.0
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015086445331750933
2	0.0037487530824350805
3	7.314640160848938E-4
4	9.143300201061172E-5
5	0.0
6	3.657320080424469E-4
7	9.143300201061172E-5
8	9.143300201061172E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	9.143300201061172E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1093697.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.018562447839784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.7293325976456	50.48024947377545
2	10.947245377990633	13.359703514574939
3	2.7722223073209062	5.074710599356656
4	1.1499422142257383	2.8067128324056143
5	0.6179674128396078	1.88537415855418
6	0.3709964185865089	1.3582600881267481
7	0.24721364272763852	1.0559234767714052
8	0.17951485571968595	0.8762990747237285
9	0.1292640055387528	0.7098753414801866
>10	0.7136771759403902	8.089351450120322
>50	0.06831271263479752	2.9105066692343518
>100	0.06858343324757017	8.431071239953559
>500	0.00482344891134937	2.0687011279380245
>1k	9.043966708780069E-4	0.8932609529847989
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3003	0.274573305037867	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2388	0.2183420088013408	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1128	0.10313642626797001	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1127	0.1030449932659594	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8286600402122344E-4
2	0.0	0.0	0.0	0.0	1.8286600402122344E-4
3	0.0	0.0	0.0	0.0	1.8286600402122344E-4
4	0.0	0.0	0.0	0.0	1.8286600402122344E-4
5	0.0	0.0	0.0	0.0	1.8286600402122344E-4
6	0.0	0.0	0.0	0.0	1.8286600402122344E-4
7	0.0	0.0	0.0	0.0	1.8286600402122344E-4
8	0.0	0.0	0.0	0.0	1.8286600402122344E-4
9	0.0	0.0	0.0	0.0	1.8286600402122344E-4
10	0.0	0.0	0.0	0.0	1.8286600402122344E-4
11	0.0	0.0	0.0	0.0	1.8286600402122344E-4
12	0.0	0.0	0.0	0.0	6.40031014074282E-4
13	0.0	0.0	0.0	0.0	8.228970180955054E-4
14	0.0	0.0	0.0	0.0	8.228970180955054E-4
15	0.0	0.0	0.0	0.0	0.0010971960241273405
16	0.0	0.0	0.0	9.143300201061172E-5	0.0011886290261379523
17	0.0	0.0	0.0	9.143300201061172E-5	0.0011886290261379523
18	0.0	0.0	0.0	9.143300201061172E-5	0.0013714950301591756
19	0.0	0.0	0.0	1.8286600402122344E-4	0.0013714950301591756
20	0.0	0.0	0.0	1.8286600402122344E-4	0.0017372270382016225
21	0.0	0.0	0.0	1.8286600402122344E-4	0.0018286600402122343
22	0.0	0.0	0.0	2.742990060318351E-4	0.001920093042222846
23	0.0	0.0	0.0	4.571650100530586E-4	0.001920093042222846
24	0.0	0.0	0.0	9.143300201061172E-4	0.001920093042222846
25	0.0	0.0	0.0	0.0011886290261379523	0.001920093042222846
26	0.0	0.0	0.0	0.0017372270382016225	0.0021029590462440695
27	0.0	0.0	0.0	0.0023772580522759047	0.0021029590462440695
28	0.0	0.0	0.0	0.003565887078413857	0.0021029590462440695
29	0.0	0.0	0.0	0.005760279126668538	0.0021029590462440695
30	0.0	0.0	0.0	0.0117034242573583	0.0021029590462440695
31	0.0	0.0	0.0	0.022218219488578647	0.0021029590462440695
32	0.0	0.0	0.0	0.03794469583440386	0.0021029590462440695
33	0.0	0.0	0.0	0.055682698224462535	0.0021029590462440695
34	0.0	0.0	0.0	0.07854094872711546	0.002194392048254681
35	0.0	0.0	0.0	0.10871383939061732	0.002194392048254681
36	0.0	0.0	0.0	0.14967582429137138	0.002194392048254681
37	0.0	0.0	0.0	0.21377035870081018	0.002194392048254681
38	0.0	0.0	0.0	0.30803778377375085	0.002285825050265293
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGAT	60	2.8726767E-4	21.99984	3
CCGATCA	75	5.4723318E-5	20.533184	9
ATCACGC	65	4.931993E-4	20.307543	3
GTGTACG	70	8.108074E-4	18.86218	1
GCACCGT	70	8.1230444E-4	18.857006	6
GTAGTAC	70	8.1230444E-4	18.857006	3
CCGTCGT	200	0.0	18.699865	9
ACCGTCG	190	1.8189894E-12	18.526182	8
TACCGTC	190	1.8189894E-12	18.526182	7
GGTATAC	60	0.007400253	18.33823	1
TATGCGT	60	0.0074119726	18.3332	39
TTAGGAC	180	1.4551915E-11	18.333199	3
ATACCGT	230	0.0	18.17378	6
GTATAGG	100	2.4769706E-5	17.6047	1
CGTCGTA	215	0.0	17.395222	10
ACGATTT	90	2.2152522E-4	17.110985	31
GGTATAT	120	6.3891694E-6	16.504408	1
TATAGGG	80	0.0019876764	16.500635	2
ACGATTA	80	0.0019882838	16.49988	32
TAGGACG	135	1.0852964E-6	16.296177	4
>>END_MODULE