FastQCFastQC Report
Thu 2 Feb 2017
SRR4062580_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062580_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910047
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG58490.6427140576255952No Hit
TCCATGTACTCTGCGTTGATACCAC47810.525357481536668No Hit
GAGTACATGGAAGCAGTGGTATCAA27440.30152288837829255No Hit
CATGTACTCTGCGTTGATACCACTG22630.24866847536445918No Hit
GTATCAACGCAGAGTACTTTTTTTT16590.18229827690218198No Hit
GCTTCCATGTACTCTGCGTTGATAC15700.1725185622281047No Hit
GTCTTGCGCCGGTCCAAGAATTTCA14340.15757427913063832No Hit
GCGTTGATACCACTGCTTCCATGTA13560.14900329323650316No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG13300.14614629793845813No Hit
GCGCAAGACGGACCAGAGCGAAAGC13090.14383872481311405No Hit
GAATAACGCCGCCGCATCGCCAGTC12570.13812473421702395No Hit
CATGGAAGCAGTGGTATCAACGCAG12490.1372456587407024No Hit
GAACTACGACGGTATCTGATCGTCT12380.13603692996076028No Hit
GAATAGGACCGCGGTTCTATTTTGT12230.13438866344265737No Hit
TATCAACGCAGAGTACTTTTTTTTT11920.13098224597191135No Hit
CCCATGTACTCTGCGTTGATACCAC11600.12746594406662515No Hit
GTATCTGATCGTCTTCGAACCTCCG11340.1246089487685801No Hit
GGGTAGGCACACGCTGAGCCAGTCA10960.12043334025605272No Hit
GTACATGGGAAGCAGTGGTATCAAC10910.11988391808335173No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10280.11296119870731951No Hit
GTCCTATTCCATTATTCCTAGCTGC10270.11285131427277932No Hit
GTATCAACGCAGAGTACATGGAAGC9930.10911524349841273No Hit
GATTAAGAGGGACGGCCGGGGGCAT9340.10263206186054126No Hit
CAGTGGTATCAACGCAGAGTACATG9210.10120356421151876No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTATA350.002181213616.2723311
CGAACGA851.0168151E-915.64796516
TCGCGTA851.7083948E-814.5326529
CGCGTAA851.7096681E-814.53185310
GCGTAAC851.7096681E-814.53185311
TTTGGTA602.563215E-514.25160917
TTAATAC400.00528640814.2461223
GTTCAAA3100.014.085191
TAACGAA954.8858055E-914.00081113
TAGGACG551.9641864E-413.8136634
GTCGCGT903.621608E-813.7252848
CGTAAAT707.275039E-613.5677353
GGTCTAC707.3164847E-613.5602761
AGGCCCG3100.013.486135510
CGGTCCA2350.013.34266710
ATAACGA1001.00335455E-813.30150312
GTACTAG500.001508025913.289071
AAGACGG1800.013.1952095
TCGGCGT1301.4551915E-1113.15460813
CCCCCGT655.4728458E-513.1473732
Produced by FastQC (version 0.11.5)