##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062580_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	910047
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.180545620171266	32.0	32.0	32.0	32.0	32.0
2	30.807552796723687	32.0	32.0	32.0	32.0	32.0
3	30.801425640653726	32.0	32.0	32.0	32.0	32.0
4	30.87144510118708	32.0	32.0	32.0	32.0	32.0
5	30.64458209301278	32.0	32.0	32.0	32.0	32.0
6	34.30604683054831	36.0	36.0	36.0	32.0	36.0
7	34.18306087487789	36.0	36.0	36.0	32.0	36.0
8	34.175816194108656	36.0	36.0	36.0	32.0	36.0
9	34.35994734337897	36.0	36.0	36.0	32.0	36.0
10	34.010615935220926	36.0	36.0	36.0	32.0	36.0
11	34.35172139460929	36.0	36.0	36.0	32.0	36.0
12	34.09384570247471	36.0	36.0	36.0	32.0	36.0
13	34.23721082537495	36.0	36.0	36.0	32.0	36.0
14	34.12866148671442	36.0	36.0	36.0	32.0	36.0
15	34.06713719181537	36.0	36.0	36.0	32.0	36.0
16	34.06084630793794	36.0	36.0	36.0	32.0	36.0
17	33.96052621458013	36.0	36.0	36.0	32.0	36.0
18	33.980988893980204	36.0	36.0	36.0	32.0	36.0
19	33.98540405056003	36.0	36.0	36.0	32.0	36.0
20	33.98699627601651	36.0	36.0	36.0	32.0	36.0
21	33.9351055494936	36.0	36.0	36.0	32.0	36.0
22	33.884236748211904	36.0	36.0	36.0	32.0	36.0
23	33.83781057461867	36.0	36.0	36.0	32.0	36.0
24	33.79666105157206	36.0	36.0	36.0	32.0	36.0
25	33.29544847683691	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	6.0
4	36.0
5	77.0
6	161.0
7	43.0
8	139.0
9	164.0
10	126.0
11	46.0
12	77.0
13	44.0
14	138.0
15	196.0
16	322.0
17	381.0
18	528.0
19	827.0
20	1212.0
21	1743.0
22	2657.0
23	3901.0
24	5346.0
25	7765.0
26	10884.0
27	14191.0
28	19370.0
29	26256.0
30	34008.0
31	46784.0
32	66344.0
33	95589.0
34	206980.0
35	363705.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.39986548947982	16.907334999329645	11.052061285806284	25.640738225384247
2	16.711043224562232	19.701103827958878	36.37555424910578	27.212298698373104
3	19.89720845233556	23.234142530140208	27.523387439738812	29.34526157778542
4	12.614927433340439	14.657190396539482	34.99695578302316	37.730926387096915
5	14.712839768899988	36.1977540737093	33.42969828609619	15.659707871294529
6	35.55570946733332	33.96624055091622	16.484940831915146	13.993109149835314
7	30.858823050760982	30.005970845121993	20.1646325841004	18.970573520016625
8	29.02007107198596	32.232608425472854	19.357162575793073	19.390157926748124
9	26.82508307456747	14.854205646540677	18.489577317920677	39.83113396097118
10	16.182668938953025	26.3744013519297	30.925832001170615	26.517097707946657
11	38.12290001254217	21.109013415715552	20.921871114954442	19.846215456787842
12	24.427486795774648	23.728983274647888	28.03411091549296	23.809419014084508
13	29.770288572299304	18.89070911397335	25.24339245458636	26.095609859140982
14	23.756402145509558	19.47000412597992	24.230284692614497	32.54330903589602
15	25.634128524342	26.375412007516246	22.70573972126509	25.284719746876664
16	26.625272556453016	25.506113405486992	23.482539885102742	24.386074152957253
17	24.163559365857576	25.707215796127326	25.467030337304884	24.662194500710214
18	25.715606071442327	23.86259483190203	26.625662241636363	23.79613685501928
19	25.36465027024172	26.027380679917606	24.714353620534848	23.89361542930582
20	25.015182787559798	23.964049799543197	25.966747494840053	25.05401991805696
21	26.35160189650638	24.710369057325416	23.693676148098266	25.244352898069934
22	25.035492369965862	24.843998652940748	24.73240403853646	25.388104938556932
23	23.832485298778124	24.34251646184725	25.202251839853517	26.62274639952111
24	24.061570896318905	25.98728441493564	24.73124077085759	25.219903917887866
25	25.130058757509737	24.37996522963843	24.61884641623204	25.87112959661979
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	92.0
1	92.0
2	272.0
3	452.0
4	452.0
5	452.0
6	1257.0
7	2062.0
8	2062.0
9	2062.0
10	2148.0
11	2234.0
12	2234.0
13	2234.0
14	2332.0
15	2430.0
16	2430.0
17	2430.0
18	3816.5
19	5203.0
20	5203.0
21	5203.0
22	8400.5
23	11598.0
24	11598.0
25	11598.0
26	17970.5
27	24343.0
28	24343.0
29	24343.0
30	29570.0
31	34797.0
32	34797.0
33	34797.0
34	43392.5
35	51988.0
36	51988.0
37	51988.0
38	59214.5
39	66441.0
40	66441.0
41	66441.0
42	78063.0
43	89685.0
44	89685.0
45	89685.0
46	112591.5
47	135498.0
48	135498.0
49	135498.0
50	129751.5
51	124005.0
52	124005.0
53	124005.0
54	113228.5
55	102452.0
56	102452.0
57	102452.0
58	95605.0
59	88758.0
60	88758.0
61	88758.0
62	79467.5
63	70177.0
64	70177.0
65	70177.0
66	58172.0
67	46167.0
68	46167.0
69	46167.0
70	34752.0
71	23337.0
72	23337.0
73	23337.0
74	18226.0
75	13115.0
76	13115.0
77	13115.0
78	10980.0
79	8845.0
80	8845.0
81	8845.0
82	6151.5
83	3458.0
84	3458.0
85	3458.0
86	2586.0
87	1714.0
88	1714.0
89	1714.0
90	1162.5
91	611.0
92	611.0
93	611.0
94	364.5
95	118.0
96	118.0
97	118.0
98	292.5
99	467.0
100	467.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00890063919775572
2	0.003516301905286211
3	0.005494221727009704
4	0.013735554317524261
5	0.034174059142000356
6	0.04779972902498442
7	0.06900742489124188
8	0.0909843117992807
9	0.10153321751513933
10	0.12351010442317814
11	0.12230137564323601
12	0.137025889871622
13	0.12351010442317814
14	0.12878455728110746
15	0.11900484260703019
16	0.11658738504714591
17	0.1281252506738663
18	0.13208109031731327
19	0.13526773891897892
20	0.12340021998863794
21	0.1338392412699564
22	0.1539480927908119
23	0.14054219177690824
24	0.13636658326438086
25	0.13482820118081812
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	910047.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.49396038016112
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.18471694803115	39.68659203720287
2	11.722383242196091	11.603743435006358
3	3.3667781841300615	4.999055581623722
4	1.392252733301344	2.75632406484751
5	0.761863980009787	1.8853832820838128
6	0.5082970339352624	1.5094579955367171
7	0.3370350248895512	1.1676838718027045
8	0.2471017588674292	0.9784035730602133
9	0.17346151942942503	0.7726767813110259
>10	1.0414442197770495	9.828094428375328
>50	0.1254504585017816	4.352360222603004
>100	0.12411663060134649	12.869799274436348
>500	0.010435566433765047	3.5254801425946405
>1k	0.004440666567559594	3.4221806402711796
>5k	2.2203332837797974E-4	0.6427646692446012
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	5849	0.6427140576255952	No Hit
TCCATGTACTCTGCGTTGATACCAC	4781	0.525357481536668	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2744	0.30152288837829255	No Hit
CATGTACTCTGCGTTGATACCACTG	2263	0.24866847536445918	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1659	0.18229827690218198	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1570	0.1725185622281047	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1434	0.15757427913063832	No Hit
GCGTTGATACCACTGCTTCCATGTA	1356	0.14900329323650316	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1330	0.14614629793845813	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1309	0.14383872481311405	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1257	0.13812473421702395	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1249	0.1372456587407024	No Hit
GAACTACGACGGTATCTGATCGTCT	1238	0.13603692996076028	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1223	0.13438866344265737	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1192	0.13098224597191135	No Hit
CCCATGTACTCTGCGTTGATACCAC	1160	0.12746594406662515	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1134	0.1246089487685801	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1096	0.12043334025605272	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1091	0.11988391808335173	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1028	0.11296119870731951	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1027	0.11285131427277932	No Hit
GTATCAACGCAGAGTACATGGAAGC	993	0.10911524349841273	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	934	0.10263206186054126	No Hit
CAGTGGTATCAACGCAGAGTACATG	921	0.10120356421151876	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0988443454019408E-4
2	0.0	0.0	0.0	0.0	1.0988443454019408E-4
3	0.0	0.0	0.0	0.0	1.0988443454019408E-4
4	0.0	0.0	0.0	0.0	1.0988443454019408E-4
5	0.0	0.0	0.0	0.0	1.0988443454019408E-4
6	0.0	0.0	0.0	0.0	1.0988443454019408E-4
7	0.0	0.0	0.0	1.0988443454019408E-4	1.0988443454019408E-4
8	0.0	0.0	0.0	1.0988443454019408E-4	1.0988443454019408E-4
9	0.0	0.0	0.0	1.0988443454019408E-4	1.0988443454019408E-4
10	0.0	0.0	0.0	2.1976886908038815E-4	1.0988443454019408E-4
11	0.0	0.0	0.0	2.1976886908038815E-4	1.0988443454019408E-4
12	0.0	0.0	0.0	2.1976886908038815E-4	6.593066072411645E-4
13	0.0	0.0	0.0	3.2965330362058224E-4	6.593066072411645E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTATA	35	0.0021812136	16.272331	1
CGAACGA	85	1.0168151E-9	15.647965	16
TCGCGTA	85	1.7083948E-8	14.532652	9
CGCGTAA	85	1.7096681E-8	14.531853	10
GCGTAAC	85	1.7096681E-8	14.531853	11
TTTGGTA	60	2.563215E-5	14.251609	17
TTAATAC	40	0.005286408	14.246122	3
GTTCAAA	310	0.0	14.08519	1
TAACGAA	95	4.8858055E-9	14.000811	13
TAGGACG	55	1.9641864E-4	13.813663	4
GTCGCGT	90	3.621608E-8	13.725284	8
CGTAAAT	70	7.275039E-6	13.567735	3
GGTCTAC	70	7.3164847E-6	13.560276	1
AGGCCCG	310	0.0	13.4861355	10
CGGTCCA	235	0.0	13.342667	10
ATAACGA	100	1.00335455E-8	13.301503	12
GTACTAG	50	0.0015080259	13.28907	1
AAGACGG	180	0.0	13.195209	5
TCGGCGT	130	1.4551915E-11	13.154608	13
CCCCCGT	65	5.4728458E-5	13.147373	2
>>END_MODULE