FastQCFastQC Report
Thu 2 Feb 2017
SRR4062580_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062580_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910047
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA22910.2517452395315846No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21380.23493292104693492No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC15800.17361740657350666No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13540.1487835243674228No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12080.13274039692455444No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG11860.13032293936467018No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11640.1279054818047859No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA11350.12471883320312029No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10740.11801588269616844No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA10330.11351062088002048No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9700.10658790150398825No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC9690.10647801706944807No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC9350.10274194629508145No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAACG300.005744557429.3330437
CGTACAC701.1313132E-625.1439863
TAGGGGT551.5927106E-424.001084
ATACGAA4400.020.9997940
CGCAATA4400.020.49979436
CGAATGC4400.019.999843
CAATACG4550.019.82397838
TAACGCC3450.019.1312944
CGATTAA1051.7918665E-618.85695533
TATGCGT1051.7918665E-618.85695539
TACGAAT4700.018.72321741
GATATAC1658.185452E-1118.6695581
ATAACGC3900.018.616223
GTATAGG1203.1546915E-718.3361721
TAGGACG1203.1583477E-718.3341584
AATAACG3600.018.3341582
TAGCTCG600.007411157218.3331510
CGGTCCA5300.018.2639710
CTAGCGG4950.018.22203829
GGTTAGT851.4286449E-418.1204531
Produced by FastQC (version 0.11.5)