##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062580_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	910047
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.245356558507417	32.0	32.0	32.0	32.0	32.0
2	31.366321739426645	32.0	32.0	32.0	32.0	32.0
3	31.48664189871512	32.0	32.0	32.0	32.0	32.0
4	31.595095637917602	32.0	32.0	32.0	32.0	32.0
5	31.51447892251719	32.0	32.0	32.0	32.0	32.0
6	35.12523089466808	36.0	36.0	36.0	36.0	36.0
7	35.13696874996566	36.0	36.0	36.0	36.0	36.0
8	35.07952666180977	36.0	36.0	36.0	36.0	36.0
9	35.20905403786838	36.0	36.0	36.0	36.0	36.0
10	35.086980123004636	36.0	36.0	36.0	36.0	36.0
11	35.221753381968185	36.0	36.0	36.0	36.0	36.0
12	35.11930372826898	36.0	36.0	36.0	36.0	36.0
13	35.167577059206835	36.0	36.0	36.0	36.0	36.0
14	35.12999768143843	36.0	36.0	36.0	36.0	36.0
15	35.082190260503026	36.0	36.0	36.0	36.0	36.0
16	35.0812892081398	36.0	36.0	36.0	36.0	36.0
17	35.06023315279321	36.0	36.0	36.0	36.0	36.0
18	35.0734621398675	36.0	36.0	36.0	36.0	36.0
19	35.058220069952434	36.0	36.0	36.0	36.0	36.0
20	35.05833764629739	36.0	36.0	36.0	36.0	36.0
21	35.03294994654122	36.0	36.0	36.0	36.0	36.0
22	35.00843363035096	36.0	36.0	36.0	36.0	36.0
23	34.95669784088074	36.0	36.0	36.0	32.0	36.0
24	34.923487468229666	36.0	36.0	36.0	32.0	36.0
25	34.8865091583182	36.0	36.0	36.0	32.0	36.0
26	34.84473768937209	36.0	36.0	36.0	32.0	36.0
27	34.80793409571154	36.0	36.0	36.0	32.0	36.0
28	34.783594693460884	36.0	36.0	36.0	32.0	36.0
29	34.75293913391286	36.0	36.0	36.0	32.0	36.0
30	34.73050402891279	36.0	36.0	36.0	32.0	36.0
31	34.73333465194655	36.0	36.0	36.0	32.0	36.0
32	34.68957097820223	36.0	36.0	36.0	32.0	36.0
33	34.65814732645676	36.0	36.0	36.0	32.0	36.0
34	34.63610011351062	36.0	36.0	36.0	32.0	36.0
35	34.583696226678406	36.0	36.0	36.0	32.0	36.0
36	34.557177816090814	36.0	36.0	36.0	32.0	36.0
37	34.516594197882085	36.0	36.0	36.0	32.0	36.0
38	34.462073936840625	36.0	36.0	36.0	32.0	36.0
39	34.399110155849094	36.0	36.0	36.0	32.0	36.0
40	34.354509162713576	36.0	36.0	36.0	32.0	36.0
41	34.33419372845578	36.0	36.0	36.0	32.0	36.0
42	34.23298467002254	36.0	36.0	36.0	32.0	36.0
43	34.27313754124787	36.0	36.0	36.0	32.0	36.0
44	34.226370725907564	36.0	36.0	36.0	32.0	36.0
45	34.11948723527466	36.0	36.0	36.0	32.0	36.0
46	34.193042776911525	36.0	36.0	36.0	32.0	36.0
47	34.13384253780299	36.0	36.0	36.0	32.0	36.0
48	34.110374519118245	36.0	36.0	36.0	32.0	36.0
49	34.151259220677616	36.0	36.0	36.0	32.0	36.0
50	33.57508678123218	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	9.0
21	24.0
22	76.0
23	253.0
24	651.0
25	1633.0
26	3582.0
27	6603.0
28	11045.0
29	16354.0
30	23311.0
31	32545.0
32	48740.0
33	74043.0
34	161166.0
35	530009.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.842999058137224	17.600854600345752	11.529411506112217	26.02673483540481
2	16.111602556001824	20.11703103794992	36.05259253968341	27.718773866364838
3	19.33257805434712	23.3496586410033	27.996712228816566	29.321051075833015
4	12.415539890884517	15.199687927520067	35.310671450312896	37.07410073128251
5	14.43145244146731	36.48053342299903	33.16169384658155	15.926320288952109
6	35.763263153267985	34.277284514966375	16.191925629642654	13.767526702122984
7	30.898953570529876	29.949881709406217	20.09434677549621	19.056817944567698
8	28.9432304045835	32.431511779061964	19.224611476110574	19.400646340243966
9	26.83729521662068	14.609025687684262	18.35070056821241	40.20297852748265
10	16.13696874996566	26.307102819964246	30.97180695063002	26.58412147944007
11	38.26241941350282	20.99419040994586	20.929468478001688	19.813921698549635
12	24.35852214226298	23.712181898297562	27.938557019582504	23.990738939856953
13	29.920762334253066	18.72309891686913	25.207379399085983	26.148759349791824
14	23.759651974018926	19.374164191519778	24.257428462486004	32.60875537197529
15	25.77504238792062	26.408306384175763	22.491036177252383	25.32561505065123
16	26.709829272554053	25.455937990015897	23.329344528359524	24.504888209070522
17	24.182926815867752	25.728890925413744	25.300671284010605	24.7875109747079
18	25.814381015486017	23.80701216530575	26.539398514582214	23.839208304626027
19	25.623511752689698	25.768998744020916	24.73212921969964	23.87536028358975
20	25.08881409421711	23.901842432313934	25.946022568065164	25.063320905403785
21	26.475226004810743	24.662242719332077	23.697677152938255	25.16485412291893
22	25.097165311242165	24.708284297404422	24.82091584280812	25.373634548545294
23	24.004364609739937	24.110073435767603	25.14419584922537	26.74136610526709
24	24.143588188302363	25.90987058910144	24.727074535710795	25.2194666868854
25	25.239465653971717	24.217430528313372	24.630046580011804	25.913057237703107
26	24.190398957416484	25.032773032601614	25.461432211742913	25.315395798238992
27	25.785591293636482	24.868166149660404	24.773006229348592	24.573236327354522
28	24.684109721805576	23.838329229149704	25.595161568578327	25.882399480466393
29	24.070075501594975	25.10474733722544	25.91404619761397	24.91113096356562
30	25.101780457492858	24.509613239755748	26.05019301200927	24.338413290742125
31	24.514997577048216	24.745205467409924	24.884868583710514	25.854928371831342
32	24.686856832669083	24.72015181633476	24.919262411721594	25.673728939274564
33	24.04645034816883	24.321710856692018	25.446048390907283	26.185790404231867
34	24.586422459499342	24.983215152623988	25.99140483953026	24.438957548346405
35	26.053050007307316	24.355665146964935	25.408687683163617	24.182597162564132
36	24.416431239265666	25.2497947908185	25.035739912334197	25.298034057581642
37	25.897233879129324	24.68092307320391	25.17265591777128	24.24918712989549
38	24.779049873248304	24.48730669954409	25.097824617849408	25.635818809358195
39	25.13430625011675	24.52367844737689	24.992005907387203	25.350009395119155
40	25.555383403274778	24.77047888735417	24.91058154139292	24.763556167978138
41	23.83745015367338	24.977611046462435	26.102498002850403	25.08244079701378
42	26.331167511128545	25.156612790328413	24.975852895509792	23.53636680303325
43	25.071122700256144	23.59152878917243	25.074858771030506	26.26248973954092
44	24.46906588341042	24.6636712169811	25.73746191130788	25.129800988300605
45	24.93025085517561	24.920251371632453	25.188699045214147	24.96079872797779
46	24.445081776265763	24.459586569440596	25.846442589589074	25.248889064704567
47	25.152547066250424	24.076119145494683	26.260511819719202	24.51082196853569
48	25.584612662862465	26.002393282984286	24.07458076341112	24.338413290742125
49	24.002936112090914	24.977501162027895	25.955472629435622	25.064090096445568
50	24.06963596385681	26.045577865758577	24.32819403832989	25.556592132054718
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11.0
1	15.5
2	20.0
3	66.5
4	113.0
5	134.5
6	156.0
7	145.5
8	135.0
9	181.0
10	227.0
11	324.5
12	422.0
13	773.0
14	1124.0
15	1522.5
16	1921.0
17	2213.0
18	2505.0
19	2649.5
20	2794.0
21	3405.5
22	4017.0
23	4109.0
24	4201.0
25	5091.5
26	5982.0
27	7666.0
28	9350.0
29	10344.0
30	11338.0
31	11855.0
32	12372.0
33	14057.5
34	15743.0
35	18242.5
36	20742.0
37	26032.0
38	31322.0
39	34154.0
40	36986.0
41	41176.0
42	45366.0
43	46889.0
44	48412.0
45	55715.0
46	63018.0
47	69360.5
48	75703.0
49	74860.0
50	74017.0
51	68902.5
52	63788.0
53	62395.0
54	61002.0
55	63017.0
56	65032.0
57	63162.5
58	61293.0
59	56236.5
60	51180.0
61	45519.0
62	39858.0
63	34686.0
64	29514.0
65	25242.5
66	20971.0
67	17943.5
68	14916.0
69	13982.0
70	13048.0
71	10071.0
72	7094.0
73	6531.0
74	5968.0
75	4206.5
76	2445.0
77	2205.0
78	1965.0
79	1746.5
80	1528.0
81	1248.0
82	968.0
83	838.5
84	709.0
85	584.0
86	459.0
87	338.5
88	218.0
89	141.0
90	64.0
91	36.0
92	8.0
93	8.5
94	9.0
95	9.5
96	10.0
97	5.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016262896311948723
2	0.003516301905286211
3	8.790754763215527E-4
4	2.1976886908038818E-4
5	0.0
6	7.691910417813587E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	3.2965330362058224E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	910047.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.564591451881505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.81114268464319	43.28615842662615
2	11.55897198147198	12.383032235825839
3	3.2286514465630494	5.1882418702702795
4	1.332457022910733	2.8549006423761494
5	0.7373475597672087	1.9747860398486152
6	0.45908221263389765	1.4754330697536313
7	0.3085059683785193	1.1567497309663723
8	0.21118038267687012	0.9049432736590833
9	0.16695827799368257	0.804874675521714
>10	0.9607228125109755	9.746983217703226
>50	0.10703677994779154	4.037046927201114
>100	0.10723950356057729	11.622218643130468
>500	0.008645027145042661	3.003102000168678
>1k	0.002058339796438729	1.561529246948711
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	2291	0.2517452395315846	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2138	0.23493292104693492	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1580	0.17361740657350666	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1354	0.1487835243674228	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1208	0.13274039692455444	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1186	0.13032293936467018	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1164	0.1279054818047859	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1135	0.12471883320312029	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	1074	0.11801588269616844	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1033	0.11351062088002048	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	970	0.10658790150398825	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	969	0.10647801706944807	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	935	0.10274194629508145	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0988443454019408E-4
2	0.0	0.0	0.0	0.0	1.0988443454019408E-4
3	0.0	0.0	0.0	0.0	1.0988443454019408E-4
4	0.0	0.0	0.0	0.0	1.0988443454019408E-4
5	0.0	0.0	0.0	0.0	1.0988443454019408E-4
6	0.0	0.0	0.0	0.0	1.0988443454019408E-4
7	0.0	0.0	0.0	0.0	1.0988443454019408E-4
8	0.0	0.0	0.0	0.0	1.0988443454019408E-4
9	0.0	0.0	0.0	0.0	1.0988443454019408E-4
10	0.0	0.0	0.0	0.0	1.0988443454019408E-4
11	0.0	0.0	0.0	0.0	1.0988443454019408E-4
12	0.0	0.0	0.0	0.0	3.2965330362058224E-4
13	0.0	0.0	0.0	0.0	3.2965330362058224E-4
14	0.0	0.0	0.0	0.0	3.2965330362058224E-4
15	0.0	0.0	0.0	0.0	5.494221727009704E-4
16	0.0	0.0	0.0	1.0988443454019408E-4	5.494221727009704E-4
17	0.0	0.0	0.0	4.395377381607763E-4	5.494221727009704E-4
18	0.0	0.0	0.0	4.395377381607763E-4	5.494221727009704E-4
19	0.0	0.0	0.0	5.494221727009704E-4	5.494221727009704E-4
20	0.0	0.0	0.0	0.0010988443454019409	5.494221727009704E-4
21	0.0	0.0	0.0	0.001538382083562717	5.494221727009704E-4
22	0.0	0.0	0.0	0.002747110863504852	5.494221727009704E-4
23	0.0	0.0	0.0	0.003736070774366599	5.494221727009704E-4
24	0.0	0.0	0.0	0.00538433729246951	5.494221727009704E-4
25	0.0	0.0	0.0	0.007362257114193003	5.494221727009704E-4
26	0.0	0.0	0.0	0.009559945804996885	5.494221727009704E-4
27	0.0	0.0	0.0	0.012526825537582125	5.494221727009704E-4
28	0.0	0.0	0.0	0.018460585002752605	5.494221727009704E-4
29	0.0	0.0	0.0	0.0296687973258524	5.494221727009704E-4
30	0.0	0.0	0.0	0.04922822667400695	5.494221727009704E-4
31	0.0	0.0	0.0	0.08702847215583372	5.494221727009704E-4
32	0.0	0.0	0.0	0.13636658326438086	5.494221727009704E-4
33	0.0	0.0	0.0	0.17471625091890858	5.494221727009704E-4
34	0.0	0.0	0.0	0.23097708140348794	6.593066072411645E-4
35	0.0	0.0	0.0	0.29075421379335353	6.593066072411645E-4
36	0.0	0.0	0.0	0.382178063330795	6.593066072411645E-4
37	0.0	0.0	0.0	0.5103033140046613	6.593066072411645E-4
38	0.0	0.0	0.0	0.6690863219152418	6.593066072411645E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAACG	30	0.0057445574	29.33304	37
CGTACAC	70	1.1313132E-6	25.143986	3
TAGGGGT	55	1.5927106E-4	24.00108	4
ATACGAA	440	0.0	20.99979	40
CGCAATA	440	0.0	20.499794	36
CGAATGC	440	0.0	19.9998	43
CAATACG	455	0.0	19.823978	38
TAACGCC	345	0.0	19.131294	4
CGATTAA	105	1.7918665E-6	18.856955	33
TATGCGT	105	1.7918665E-6	18.856955	39
TACGAAT	470	0.0	18.723217	41
GATATAC	165	8.185452E-11	18.669558	1
ATAACGC	390	0.0	18.61622	3
GTATAGG	120	3.1546915E-7	18.336172	1
TAGGACG	120	3.1583477E-7	18.334158	4
AATAACG	360	0.0	18.334158	2
TAGCTCG	60	0.0074111572	18.33315	10
CGGTCCA	530	0.0	18.26397	10
CTAGCGG	495	0.0	18.222038	29
GGTTAGT	85	1.4286449E-4	18.120453	1
>>END_MODULE