FastQCFastQC Report
Thu 2 Feb 2017
SRR4062579_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062579_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32656
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTACATGGGAGAAATCGT5701.74546790788829No Hit
ACGCAGAGTACTTTTTTTTTTTTTT2730.8359872611464968No Hit
CTCCGAGGTCACCCCAACCGAAATT1570.4807692307692308No Hit
GTATCAACGCAGAGTACTTTTTTTT1240.37971582557569816No Hit
ACGCAGAGTACATGGGACTAATCCT1180.36134247917687407No Hit
CCATTGGGATGTCCTGATCCAACAT1120.34296913277804997No Hit
CCATAGGGTCTTCTCGTCTTATTAT1060.32459578637922587No Hit
ATCTATAACTTTATAGATGCAACAC930.28478686918177365No Hit
GTCCAACTGTCTCTTATCTTTAATC930.28478686918177365No Hit
TATCAACGCAGAGTACTTTTTTTTT930.28478686918177365No Hit
GTTATAGATTAACCCAATTTTAAGT870.26641352278294955No Hit
AAGAGGGACAGCTCTTCTGGAACGG830.25416462518373345No Hit
GTACATGGAAGCAGTGGTATCAACG790.24191572758451738No Hit
TCCATGTACTCTGCGTTGATACCAC780.23885350318471338No Hit
CCGCAAGGGAAAGATGAAAGACTAA750.2296668299853013No Hit
GTCCTACAGTGGACATTTCTAAATT730.2235423811856933No Hit
CTGTTAGTATGAGTAACAAGAATTC710.21741793238608526No Hit
GTCCTAAAGTGTGTATTTCTCATTT690.21129348358647723No Hit
CTTTTAGGCCTACAATGGTTAAAAG690.21129348358647723No Hit
GTATCCTGACCGTGCAAAGGTAGCA670.20516903478686918No Hit
CCAATAAAGAAAGCGTTCAAGCTCA660.20210681038706516No Hit
CTGTAGGACGTGGAATATGGCAAGA650.19904458598726116No Hit
GAGTACTTTTTTTTTTTTTTTTTTT630.1929201371876531No Hit
TACTAACAGTGTTGCATCTATAAAG620.18985791278784908No Hit
AAATATATCTGGGTCAATAAGATAT620.18985791278784908No Hit
GTCCTACAGTGTGCATTTCTCATTT600.18373346398824106No Hit
CTTCTACACCATTGGGATGTCCTGA590.18067123958843703No Hit
TTGTAGAACAGTGTATATCAATGAG590.18067123958843703No Hit
CTTTAGGACGTGAAATATGGCGAGG590.18067123958843703No Hit
ATCCTAGCCCTAGCCCTACACAAAT580.17760901518863303No Hit
CTATAGAACTAGTACCGCAAGGGAA570.174546790788829No Hit
ACGCAGAGTACATGGAAGCAGTGGT560.17148456638902498No Hit
ATCCTGACCGTGCAAAGGTAGCATA550.16842234198922096No Hit
CCTATAGTCTGATTAACTAACAATG550.16842234198922096No Hit
GTATTAGAGGCACTGCCTGCCCAGT540.16536011758941693No Hit
GATATACACTGTTCTACAAATCCCG540.16536011758941693No Hit
CCTTTAGGCATTCCGGTGTTGGGTT540.16536011758941693No Hit
CTATTATATAAATCAAAACATTTAT530.16229789318961294No Hit
GATTAAACCTTGTACCTTTTGCATA510.1561734443900049No Hit
GTACTTTTTTTTTTTTTTTTTTTTT500.1531112199902009No Hit
CCTCTAGCATTACAAGTATTAGAGG500.1531112199902009No Hit
GTTATATAATTTAAGCTCCATAGGG500.1531112199902009No Hit
GATATATTTTGATCAACGGACCAAG490.15004899559039686No Hit
ATTTTAAACTTGCTAAAAAAACAAC470.1439245467907888No Hit
GTGTAGAAGCTATTAATGGTTCGTT470.1439245467907888No Hit
AGCATGAACGGCTAAACGAGGGTCC470.1439245467907888No Hit
CTCTAATACTTGTAATGCTAGAGGT460.1408623223909848No Hit
GTTATAGGTGGATTATTTATAGTGT460.1408623223909848No Hit
GGTATCAACGCAGAGTACTTTTTTT460.1408623223909848No Hit
ATCGTAAATAGATAGAAACCGACCT450.1378000979911808No Hit
GTGTATATCAATGAGTTACAATGAA440.1347378735913768No Hit
CTGTAGGACCTGGAATATGGCGAGA440.1347378735913768No Hit
ACGCAGAGTACATGGGCAGAAATCA430.13167564919157276No Hit
ATAAATAATCCACCTATAACTTCTC430.13167564919157276No Hit
TCCCAATGGTGTAGAAGCTATTAAT430.13167564919157276No Hit
GTTATACGCGTATGCCTGGAGAATT420.12861342479176874No Hit
GTTATCCCTAGGGTAACTTGGTCCG420.12861342479176874No Hit
GAGTACATGGAAGCAGTGGTATCAA420.12861342479176874No Hit
CATTTATCCTACTAAAAGTATTGGA420.12861342479176874No Hit
GTCAGGATACCGCGGCCGTTAAACT420.12861342479176874No Hit
AATCTAAACTTACTTTTTGATTTTG410.1255512003919647No Hit
ATTATAACCTAGACTTACAAGTCAA410.1255512003919647No Hit
GTACATGGGAGAAATCGTAAATAGA400.1224889759921607No Hit
GCGTATGCCTGGAGAATTGGAATTC400.1224889759921607No Hit
GTTTAAAATTGAACTTAAATTCATT380.11636452719255266No Hit
TCTTTAATCAGTGAAATTGACCTTT380.11636452719255266No Hit
GTATCAACGCAGAGTACATGGGAGA370.11330230279274864No Hit
ATTATTTATAGTGTGATTATTGCCT370.11330230279274864No Hit
CTATTAAAGGTTTTTTCCGTTCCAG370.11330230279274864No Hit
GGTTTAATCTTTGCTTGTTCTTACT360.11024007839294464No Hit
GACCTGGATTGCTCCGGTCTGAACT360.11024007839294464No Hit
ACTAAAAGTATTGGAGAAAGAAATT360.11024007839294464No Hit
ACGTAGGACTTTAATCGTTGAACAA360.11024007839294464No Hit
CCAGAAGAGCTGTCCCTCTTTTGGC360.11024007839294464No Hit
GTTAGTATGAGTAACAAGAATTCCA360.11024007839294464No Hit
GATAGAAACCGACCTGGATTGCTCC350.10717785399314063No Hit
GGTGATAGCTGGTTACCCAAAAAAT350.10717785399314063No Hit
CCTTTAATAGTGAATAATTAACAAA350.10717785399314063No Hit
CTATAGTATAAGTTTGAAATTTCGG340.1041156295933366No Hit
CTTAAATAGGATTGCGCTGTTATCC340.1041156295933366No Hit
CTTTATTGGTGGCTGCTTTTAGGCC330.10105340519353258No Hit
GATTAAAGATAAGAGACAGTTGGAC330.10105340519353258No Hit
TTTATAGTGTGATTATTGCCTATAG330.10105340519353258No Hit
ACTTTATAGATGCAACACTGTTAGT330.10105340519353258No Hit
AGGGATAACAGCGCAATCCTATTTA330.10105340519353258No Hit
CATGTACTCTGCGTTGATACCACTG330.10105340519353258No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATTT250.005903553719.00306914
TAGGACC250.005903553719.0030694
TTTTAAG250.005903553719.00306918
CCCAATT250.005903553719.00306913
ATGGGAG552.5893314E-819.00306712
AAATCGT451.5663045E-619.00306719
GAAATCG451.5663045E-619.00306718
AGGACCT307.495294E-419.0030675
GGGAGAA451.5663045E-619.00306714
TGGGAGA451.5663045E-619.00306713
TGTATAT250.005956249318.9739672
GAGAAAT504.286054E-617.10276216
AGAAATC551.06071475E-515.54796417
CATGGGA1550.015.32505511
AGTACAT2950.015.1380367
GAGTACA3000.014.88573556
TGTAGGA456.5831706E-414.7575292
CTGTAGG456.5831706E-414.7575291
ACGCAGA3350.014.442871
CGCAGAG3300.014.3742172
Produced by FastQC (version 0.11.5)