##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062579_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32656
Sequences flagged as poor quality	0
Sequence length	25
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.838375796178344	32.0	32.0	32.0	14.0	32.0
2	27.767760901518862	32.0	21.0	32.0	14.0	32.0
3	28.148027927486527	32.0	32.0	32.0	14.0	32.0
4	27.71169157275845	32.0	21.0	32.0	14.0	32.0
5	27.693471337579616	32.0	21.0	32.0	14.0	32.0
6	31.108065899069082	36.0	32.0	36.0	14.0	36.0
7	30.818471337579616	36.0	27.0	36.0	14.0	36.0
8	30.890219255267027	36.0	27.0	36.0	14.0	36.0
9	31.36596643802058	36.0	32.0	36.0	14.0	36.0
10	30.201157520823127	36.0	21.0	36.0	14.0	36.0
11	31.640402988731015	36.0	32.0	36.0	14.0	36.0
12	30.579005389514943	36.0	27.0	36.0	14.0	36.0
13	31.156020333170016	36.0	32.0	36.0	14.0	36.0
14	30.61608892699657	36.0	27.0	36.0	14.0	36.0
15	30.56455169034787	36.0	27.0	36.0	14.0	36.0
16	30.67096398824106	36.0	27.0	36.0	14.0	36.0
17	30.32128858402744	36.0	21.0	36.0	14.0	36.0
18	30.528019353258205	36.0	27.0	36.0	14.0	36.0
19	30.515739833414994	36.0	27.0	36.0	14.0	36.0
20	30.416829985301323	36.0	27.0	36.0	14.0	36.0
21	30.39202596766291	36.0	27.0	36.0	14.0	36.0
22	30.165758206761392	36.0	21.0	36.0	14.0	36.0
23	30.35840274375306	36.0	27.0	36.0	14.0	36.0
24	30.28371509064184	36.0	27.0	36.0	14.0	36.0
25	29.57049240568349	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	2.0
5	3.0
6	7.0
7	3.0
8	5.0
9	3.0
10	4.0
11	2.0
12	7.0
13	0.0
14	37.0
15	108.0
16	258.0
17	491.0
18	774.0
19	902.0
20	900.0
21	883.0
22	812.0
23	809.0
24	835.0
25	865.0
26	918.0
27	983.0
28	1160.0
29	1294.0
30	1495.0
31	1814.0
32	2292.0
33	2926.0
34	5251.0
35	6813.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.84785005512679	30.34117358814161	11.558250643145902	24.25272571358569
2	11.321043606075454	21.025232729054384	39.37101910828025	28.282704556589906
3	24.993875551200393	24.880573248407643	30.14453699167075	19.981014208721216
4	8.458640859951611	17.716595718616972	39.38076133892751	34.44400208250391
5	9.34570850946517	45.72995160203394	34.92005146112847	10.004288427372419
6	31.477850621898167	40.80632314196434	17.89106059677716	9.824765639360333
7	22.187490423217184	41.13879439796512	21.568447182127425	15.105267996690264
8	29.119666503187837	35.25931829328102	20.80983325159392	14.811181951937225
9	22.73953288788083	14.699932569116655	29.626678109483233	32.93385643351928
10	12.039733881104945	34.56173161234939	34.21222062114848	19.186313885397187
11	28.4535892796909	22.553739535739474	26.51559289810187	22.477078286467755
12	19.110702238577122	33.63385464581417	31.241950321987122	16.01349279362159
13	22.837253518967156	21.978594866447914	35.51473519580484	19.66941641878009
14	23.82251931804244	23.948239911688948	27.845578314730773	24.383662455537838
15	26.415672808658066	30.42891743569304	24.760094429285342	18.395315326363555
16	25.98031701260079	28.488211668761686	27.979274611398964	17.552196707238558
17	16.947229632355196	33.22601416613007	30.769938368135406	19.056817833379327
18	19.40687582420953	28.20560002453461	33.94363173551692	18.44389241573895
19	20.831033425329654	30.00306654400491	29.8589389757743	19.306961054891136
20	20.13737695869492	31.796019747937816	30.817822207230687	17.24878108613658
21	19.862635677929724	30.62795118660698	29.131661249770037	20.37775188569326
22	18.285539027756478	31.811071921484434	31.41542708173593	18.487961969023157
23	19.102088380508448	29.611456959735044	30.816645711306695	20.469808948449813
24	21.581674894974086	30.038943914630035	30.28119346232866	18.098187728067217
25	19.776142287641825	28.813247470101196	33.695185525912294	17.71542471634468
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	3.0
2	20.0
3	37.0
4	37.0
5	37.0
6	106.5
7	176.0
8	176.0
9	176.0
10	210.0
11	244.0
12	244.0
13	244.0
14	280.5
15	317.0
16	317.0
17	317.0
18	531.0
19	745.0
20	745.0
21	745.0
22	1260.0
23	1775.0
24	1775.0
25	1775.0
26	2351.5
27	2928.0
28	2928.0
29	2928.0
30	3381.5
31	3835.0
32	3835.0
33	3835.0
34	3891.0
35	3947.0
36	3947.0
37	3947.0
38	3734.5
39	3522.0
40	3522.0
41	3522.0
42	3410.0
43	3298.0
44	3298.0
45	3298.0
46	3941.0
47	4584.0
48	4584.0
49	4584.0
50	3785.5
51	2987.0
52	2987.0
53	2987.0
54	2450.0
55	1913.0
56	1913.0
57	1913.0
58	1415.5
59	918.0
60	918.0
61	918.0
62	739.5
63	561.0
64	561.0
65	561.0
66	454.0
67	347.0
68	347.0
69	347.0
70	285.0
71	223.0
72	223.0
73	223.0
74	180.0
75	137.0
76	137.0
77	137.0
78	105.5
79	74.0
80	74.0
81	74.0
82	56.0
83	38.0
84	38.0
85	38.0
86	23.0
87	8.0
88	8.0
89	8.0
90	7.0
91	6.0
92	6.0
93	6.0
94	4.5
95	3.0
96	3.0
97	3.0
98	16.5
99	30.0
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01224889759921607
2	0.0
3	0.0
4	0.009186673199412052
5	0.030622243998040175
6	0.042871141597256246
7	0.07655560999510044
8	0.09799118079372857
9	0.09186673199412053
10	0.11942675159235669
11	0.1378000979911808
12	0.1408623223909848
13	0.1439245467907888
14	0.1347378735913768
15	0.11942675159235669
16	0.11942675159235669
17	0.13167564919157276
18	0.15004899559039686
19	0.1408623223909848
20	0.1378000979911808
21	0.12861342479176874
22	0.1561734443900049
23	0.1439245467907888
24	0.1378000979911808
25	0.1408623223909848
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	32656.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.66144047035767
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.49250483558994	42.2985056344929
2	7.4286750483559	7.526947574718275
3	2.3815280464216637	3.619549240568349
4	1.36605415860735	2.768250857422832
5	0.9731624758220503	2.465090641842234
6	0.5379593810444875	1.6352278294953455
7	0.4654255319148936	1.6505389514943656
8	0.3566247582205029	1.4453699167074963
9	0.3505802707930368	1.5984811366976972
>10	2.4117504835589942	23.99252817246448
>50	0.19342359767891684	6.528662420382165
>100	0.03626692456479691	2.7253797158255755
>500	0.006044487427466151	1.74546790788829
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ACGCAGAGTACATGGGAGAAATCGT	570	1.74546790788829	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	273	0.8359872611464968	No Hit
CTCCGAGGTCACCCCAACCGAAATT	157	0.4807692307692308	No Hit
GTATCAACGCAGAGTACTTTTTTTT	124	0.37971582557569816	No Hit
ACGCAGAGTACATGGGACTAATCCT	118	0.36134247917687407	No Hit
CCATTGGGATGTCCTGATCCAACAT	112	0.34296913277804997	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	106	0.32459578637922587	No Hit
ATCTATAACTTTATAGATGCAACAC	93	0.28478686918177365	No Hit
GTCCAACTGTCTCTTATCTTTAATC	93	0.28478686918177365	No Hit
TATCAACGCAGAGTACTTTTTTTTT	93	0.28478686918177365	No Hit
GTTATAGATTAACCCAATTTTAAGT	87	0.26641352278294955	No Hit
AAGAGGGACAGCTCTTCTGGAACGG	83	0.25416462518373345	No Hit
GTACATGGAAGCAGTGGTATCAACG	79	0.24191572758451738	No Hit
TCCATGTACTCTGCGTTGATACCAC	78	0.23885350318471338	No Hit
CCGCAAGGGAAAGATGAAAGACTAA	75	0.2296668299853013	No Hit
GTCCTACAGTGGACATTTCTAAATT	73	0.2235423811856933	No Hit
CTGTTAGTATGAGTAACAAGAATTC	71	0.21741793238608526	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	69	0.21129348358647723	No Hit
CTTTTAGGCCTACAATGGTTAAAAG	69	0.21129348358647723	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	67	0.20516903478686918	No Hit
CCAATAAAGAAAGCGTTCAAGCTCA	66	0.20210681038706516	No Hit
CTGTAGGACGTGGAATATGGCAAGA	65	0.19904458598726116	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	63	0.1929201371876531	No Hit
TACTAACAGTGTTGCATCTATAAAG	62	0.18985791278784908	No Hit
AAATATATCTGGGTCAATAAGATAT	62	0.18985791278784908	No Hit
GTCCTACAGTGTGCATTTCTCATTT	60	0.18373346398824106	No Hit
CTTCTACACCATTGGGATGTCCTGA	59	0.18067123958843703	No Hit
TTGTAGAACAGTGTATATCAATGAG	59	0.18067123958843703	No Hit
CTTTAGGACGTGAAATATGGCGAGG	59	0.18067123958843703	No Hit
ATCCTAGCCCTAGCCCTACACAAAT	58	0.17760901518863303	No Hit
CTATAGAACTAGTACCGCAAGGGAA	57	0.174546790788829	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	56	0.17148456638902498	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	55	0.16842234198922096	No Hit
CCTATAGTCTGATTAACTAACAATG	55	0.16842234198922096	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	54	0.16536011758941693	No Hit
GATATACACTGTTCTACAAATCCCG	54	0.16536011758941693	No Hit
CCTTTAGGCATTCCGGTGTTGGGTT	54	0.16536011758941693	No Hit
CTATTATATAAATCAAAACATTTAT	53	0.16229789318961294	No Hit
GATTAAACCTTGTACCTTTTGCATA	51	0.1561734443900049	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	50	0.1531112199902009	No Hit
CCTCTAGCATTACAAGTATTAGAGG	50	0.1531112199902009	No Hit
GTTATATAATTTAAGCTCCATAGGG	50	0.1531112199902009	No Hit
GATATATTTTGATCAACGGACCAAG	49	0.15004899559039686	No Hit
ATTTTAAACTTGCTAAAAAAACAAC	47	0.1439245467907888	No Hit
GTGTAGAAGCTATTAATGGTTCGTT	47	0.1439245467907888	No Hit
AGCATGAACGGCTAAACGAGGGTCC	47	0.1439245467907888	No Hit
CTCTAATACTTGTAATGCTAGAGGT	46	0.1408623223909848	No Hit
GTTATAGGTGGATTATTTATAGTGT	46	0.1408623223909848	No Hit
GGTATCAACGCAGAGTACTTTTTTT	46	0.1408623223909848	No Hit
ATCGTAAATAGATAGAAACCGACCT	45	0.1378000979911808	No Hit
GTGTATATCAATGAGTTACAATGAA	44	0.1347378735913768	No Hit
CTGTAGGACCTGGAATATGGCGAGA	44	0.1347378735913768	No Hit
ACGCAGAGTACATGGGCAGAAATCA	43	0.13167564919157276	No Hit
ATAAATAATCCACCTATAACTTCTC	43	0.13167564919157276	No Hit
TCCCAATGGTGTAGAAGCTATTAAT	43	0.13167564919157276	No Hit
GTTATACGCGTATGCCTGGAGAATT	42	0.12861342479176874	No Hit
GTTATCCCTAGGGTAACTTGGTCCG	42	0.12861342479176874	No Hit
GAGTACATGGAAGCAGTGGTATCAA	42	0.12861342479176874	No Hit
CATTTATCCTACTAAAAGTATTGGA	42	0.12861342479176874	No Hit
GTCAGGATACCGCGGCCGTTAAACT	42	0.12861342479176874	No Hit
AATCTAAACTTACTTTTTGATTTTG	41	0.1255512003919647	No Hit
ATTATAACCTAGACTTACAAGTCAA	41	0.1255512003919647	No Hit
GTACATGGGAGAAATCGTAAATAGA	40	0.1224889759921607	No Hit
GCGTATGCCTGGAGAATTGGAATTC	40	0.1224889759921607	No Hit
GTTTAAAATTGAACTTAAATTCATT	38	0.11636452719255266	No Hit
TCTTTAATCAGTGAAATTGACCTTT	38	0.11636452719255266	No Hit
GTATCAACGCAGAGTACATGGGAGA	37	0.11330230279274864	No Hit
ATTATTTATAGTGTGATTATTGCCT	37	0.11330230279274864	No Hit
CTATTAAAGGTTTTTTCCGTTCCAG	37	0.11330230279274864	No Hit
GGTTTAATCTTTGCTTGTTCTTACT	36	0.11024007839294464	No Hit
GACCTGGATTGCTCCGGTCTGAACT	36	0.11024007839294464	No Hit
ACTAAAAGTATTGGAGAAAGAAATT	36	0.11024007839294464	No Hit
ACGTAGGACTTTAATCGTTGAACAA	36	0.11024007839294464	No Hit
CCAGAAGAGCTGTCCCTCTTTTGGC	36	0.11024007839294464	No Hit
GTTAGTATGAGTAACAAGAATTCCA	36	0.11024007839294464	No Hit
GATAGAAACCGACCTGGATTGCTCC	35	0.10717785399314063	No Hit
GGTGATAGCTGGTTACCCAAAAAAT	35	0.10717785399314063	No Hit
CCTTTAATAGTGAATAATTAACAAA	35	0.10717785399314063	No Hit
CTATAGTATAAGTTTGAAATTTCGG	34	0.1041156295933366	No Hit
CTTAAATAGGATTGCGCTGTTATCC	34	0.1041156295933366	No Hit
CTTTATTGGTGGCTGCTTTTAGGCC	33	0.10105340519353258	No Hit
GATTAAAGATAAGAGACAGTTGGAC	33	0.10105340519353258	No Hit
TTTATAGTGTGATTATTGCCTATAG	33	0.10105340519353258	No Hit
ACTTTATAGATGCAACACTGTTAGT	33	0.10105340519353258	No Hit
AGGGATAACAGCGCAATCCTATTTA	33	0.10105340519353258	No Hit
CATGTACTCTGCGTTGATACCACTG	33	0.10105340519353258	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0030622243998040177	0.0
10	0.0	0.0	0.0	0.0030622243998040177	0.0
11	0.0	0.0	0.0	0.0030622243998040177	0.0
12	0.0	0.0	0.0	0.0030622243998040177	0.0
13	0.0	0.0	0.0	0.0030622243998040177	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAATTT	25	0.0059035537	19.003069	14
TAGGACC	25	0.0059035537	19.003069	4
TTTTAAG	25	0.0059035537	19.003069	18
CCCAATT	25	0.0059035537	19.003069	13
ATGGGAG	55	2.5893314E-8	19.003067	12
AAATCGT	45	1.5663045E-6	19.003067	19
GAAATCG	45	1.5663045E-6	19.003067	18
AGGACCT	30	7.495294E-4	19.003067	5
GGGAGAA	45	1.5663045E-6	19.003067	14
TGGGAGA	45	1.5663045E-6	19.003067	13
TGTATAT	25	0.0059562493	18.973967	2
GAGAAAT	50	4.286054E-6	17.102762	16
AGAAATC	55	1.06071475E-5	15.547964	17
CATGGGA	155	0.0	15.325055	11
AGTACAT	295	0.0	15.138036	7
GAGTACA	300	0.0	14.8857355	6
TGTAGGA	45	6.5831706E-4	14.757529	2
CTGTAGG	45	6.5831706E-4	14.757529	1
ACGCAGA	335	0.0	14.44287	1
CGCAGAG	330	0.0	14.374217	2
>>END_MODULE