FastQCFastQC Report
Thu 2 Feb 2017
SRR4062579_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062579_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32656
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG1070.3276580107790299No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC1000.3062224399804018No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC860.2633512983831455No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT770.23579127878490935No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT750.2296668299853013No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT740.22660460558549728No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCTCGATGTTGGATC710.21741793238608526No Hit
AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCGACAATTAGGGTT670.20516903478686918No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA630.1929201371876531No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG630.1929201371876531No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGAC580.17760901518863303No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA580.17760901518863303No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA570.174546790788829No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA570.174546790788829No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA570.174546790788829No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC560.17148456638902498No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT550.16842234198922096No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT530.16229789318961294No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA530.16229789318961294No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT520.15923566878980894No Hit
CAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGTGTA520.15923566878980894No Hit
CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT520.15923566878980894No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.1561734443900049No Hit
AAGTTACCCTAGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCGAC510.1561734443900049No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA500.1531112199902009No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC500.1531112199902009No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC490.15004899559039686No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG480.14698677119059284No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC470.1439245467907888No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA460.1408623223909848No Hit
GTCCAACTGTCTCTTATCTTTAATCAGTGAAATTGACCTTTCAGTGAAGA450.1378000979911808No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTATCTTTAATCAGTG440.1347378735913768No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC440.1347378735913768No Hit
TTGTAGGCCTAAAAGCAGCCACCAATAAAGAAAGCGTTCAAGCTCAACAT440.1347378735913768No Hit
CTATTATATAAATCAAAACATTTATCCTACTAAAAGTATTGGAGAAAGAA440.1347378735913768No Hit
TTATATAACTTATCTATTTAATTTATTAAACCTAATGGCCCAAAAACTAT430.13167564919157276No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCA430.13167564919157276No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC430.13167564919157276No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA430.13167564919157276No Hit
GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT420.12861342479176874No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT410.1255512003919647No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA410.1255512003919647No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG390.11942675159235669No Hit
CCATTAATAGCTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTC380.11636452719255266No Hit
GGGCAGGCAGTGCCTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGT370.11330230279274864No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT370.11330230279274864No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA370.11330230279274864No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT360.11024007839294464No Hit
TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA350.10717785399314063No Hit
AATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCT350.10717785399314063No Hit
CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG350.10717785399314063No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA350.10717785399314063No Hit
CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAAAAA350.10717785399314063No Hit
GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC350.10717785399314063No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA350.10717785399314063No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG350.10717785399314063No Hit
GATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGA340.1041156295933366No Hit
GTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATCTGGGTCAAT340.1041156295933366No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC340.1041156295933366No Hit
ATATAAATCAAAACATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCG340.1041156295933366No Hit
GTTCTATAGCTCCTAGATGTACGAATTTCTTTCTCCAATACTTTTAGTAG340.1041156295933366No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA340.1041156295933366No Hit
ATTTCAGCCTCTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGA340.1041156295933366No Hit
ACATAAAATTTCAATTAATTCCATAATTTACACCAACTTCCTAAACTTAA330.10105340519353258No Hit
CTATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCTCGATGTTGGA330.10105340519353258No Hit
GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT330.10105340519353258No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACC330.10105340519353258No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGTG301.2736085E-436.6666645
GGACCAA250.0023195335.218
AGTGTGT401.7667022E-533.08
GTCCTAA353.1503197E-431.4285721
ATATATT353.1503197E-431.4285722
AAAGTGT502.2699624E-630.86
AAGTGTG453.9510072E-529.3333367
GTGTGTA453.9510072E-529.3333369
ACCAAGT300.005664569329.33333220
CGGACCA300.005664569329.33333217
GATCAAC300.005664569329.33333211
AACGGAC300.005664569329.33333215
ATTTTGA300.005664569329.3333326
GACCAAG300.005664569329.33333219
CAACGGA300.005664569329.33333214
TTTGATC300.005664569329.3333328
GTGCATT406.885688E-427.50000211
CTAATAC406.885688E-427.50000216
TAATACT406.885688E-427.50000217
GTAATGC406.885688E-427.50000225
Produced by FastQC (version 0.11.5)