FastQCFastQC Report
Thu 2 Feb 2017
SRR4062578_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062578_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868405
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34190.1829903045645885No Hit
GTCTTGCGCCGGTCCAAGAATTTCA23770.12722081133373117No Hit
GCGCAAGACGGACCAGAGCGAAAGC23130.12379542979172074No Hit
TATCAACGCAGAGTACTTTTTTTTT22330.11951370286420769No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG21550.11533901910988249No Hit
GAATAACGCCGCCGCATCGCCAGTC21110.11298406929975031No Hit
GATTAAGAGGGACGGCCGGGGGCAT20820.11143194328852686No Hit
GTATCTGATCGTCTTCGAACCTCCG20060.10736430270738946No Hit
GAACTACGACGGTATCTGATCGTCT19870.10634739256210511No Hit
GAATAGGACCGCGGTTCTATTTTGT19740.10565161193638424No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19570.10474174496428772No Hit
GGGTAGGCACACGCTGAGCCAGTCA19030.10185157928821642No Hit
GTCCTATTCCATTATTCCTAGCTGC18840.10083466914293207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATAC1152.5465852E-1114.0368443
CGCGTAA1750.013.57192610
ACGCTTA1205.0931703E-1113.46279717
GCGTAAC1850.013.35148411
CGCCAGT3250.013.15856218
GTCCTAA2600.013.1444621
TGAACGG1207.421477E-1012.6654345
ATCGCCA3400.012.57736316
AATCGTA1001.4398029E-712.35210913
TCGACGC1251.4078978E-912.16207714
TCGCCAG3600.012.14291517
CGCATCG3450.012.118010513
TCGCGTA2000.011.8754359
CGCAAGA4000.011.8668472
CAAGACG4050.011.7259964
CGCTTAC1302.6011548E-911.69649818
CGTTCAA658.01326E-411.69430414
CTCGACG1302.6084308E-911.69430413
CATCGCC3750.011.65563615
AAGACGG4100.011.5842385
Produced by FastQC (version 0.11.5)