##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062578_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1868405
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.169361567754315	32.0	32.0	32.0	32.0	32.0
2	30.73643401725001	32.0	32.0	32.0	32.0	32.0
3	30.731324846593754	32.0	32.0	32.0	32.0	32.0
4	30.819189094441516	32.0	32.0	32.0	32.0	32.0
5	30.572602835038442	32.0	32.0	32.0	32.0	32.0
6	34.24255983044361	36.0	36.0	36.0	32.0	36.0
7	34.12238353033737	36.0	36.0	36.0	32.0	36.0
8	34.10614347531718	36.0	36.0	36.0	32.0	36.0
9	34.308048843799924	36.0	36.0	36.0	32.0	36.0
10	33.91201800466173	36.0	36.0	36.0	32.0	36.0
11	34.287934896342065	36.0	36.0	36.0	32.0	36.0
12	34.01298273126009	36.0	36.0	36.0	32.0	36.0
13	34.168777647244575	36.0	36.0	36.0	32.0	36.0
14	34.049514425405626	36.0	36.0	36.0	32.0	36.0
15	33.977976937548334	36.0	36.0	36.0	32.0	36.0
16	33.98927427404658	36.0	36.0	36.0	32.0	36.0
17	33.89422528841445	36.0	36.0	36.0	32.0	36.0
18	33.91464163283656	36.0	36.0	36.0	32.0	36.0
19	33.90693238350357	36.0	36.0	36.0	32.0	36.0
20	33.909489109695166	36.0	36.0	36.0	32.0	36.0
21	33.87753297598754	36.0	36.0	36.0	32.0	36.0
22	33.828432272446285	36.0	36.0	36.0	32.0	36.0
23	33.80003478903129	36.0	36.0	36.0	32.0	36.0
24	33.75672083943257	36.0	36.0	36.0	32.0	36.0
25	33.30292415188356	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3.0
3	6.0
4	78.0
5	177.0
6	331.0
7	91.0
8	286.0
9	354.0
10	223.0
11	77.0
12	145.0
13	121.0
14	333.0
15	389.0
16	594.0
17	845.0
18	1164.0
19	1577.0
20	2502.0
21	3779.0
22	5918.0
23	8744.0
24	12443.0
25	17599.0
26	24354.0
27	31185.0
28	42212.0
29	55378.0
30	70840.0
31	95546.0
32	137623.0
33	195973.0
34	426200.0
35	731315.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.11267610911071	17.558535320311034	11.134062115445039	26.194726455133228
2	17.339914608278725	19.911782227025082	36.39890362039703	26.349399544299164
3	19.415065577912042	23.410436800705664	27.949667935548238	29.22482968583406
4	12.976612976612978	14.939110233227881	35.45454545454545	36.62973133561369
5	15.058145137974236	36.063049460845725	33.212706265326005	15.666099135854028
6	34.56936947163873	34.88160023217846	16.647880702254156	13.901149593928661
7	30.630463681148644	30.20938331167767	20.46781589864935	18.692337108524335
8	28.473102427097608	32.24644474610992	19.507851933438833	19.772600893353633
9	27.59232749069385	14.197903250347707	18.66539012958959	39.544379129368856
10	16.271202244710288	26.14111108014893	31.108647236232606	26.47903943890817
11	37.816687771593074	21.123932450659755	21.849252414026676	19.210127363720495
12	24.86059808299586	23.743774881714714	28.170281505047623	23.2253455302418
13	29.477108841787565	19.104706845697432	25.246617450354975	26.171566862160027
14	23.60615395168672	19.677457732847156	24.601605155017072	32.11478316044905
15	25.30664080291932	27.087166901548077	21.920704751391874	25.685487544140734
16	26.116039134111173	25.6646936713039	23.507312728874428	24.711954465710498
17	24.34759541698374	25.594098669896358	25.045980214574335	25.012325698545567
18	25.196556259305268	24.50980181984031	26.06574492008588	24.22789700076854
19	25.794921761947943	24.82865662938595	25.156390352033718	24.22003125663239
20	25.820443922353782	24.077894878997984	24.87960891788969	25.22205228075854
21	26.868628943221896	24.037280396171205	24.12463957638837	24.969451084218537
22	26.033424678683083	24.37468104176584	24.624862231485412	24.967032048065665
23	24.436732013882377	24.084527047851314	25.384592707733127	26.094148230533186
24	24.68779947132257	24.936273574489707	25.09652757976791	25.279399374419814
25	24.796155383888156	24.54201283896838	25.14928596770857	25.512545809434894
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	297.0
1	297.0
2	651.5
3	1006.0
4	1006.0
5	1006.0
6	2468.0
7	3930.0
8	3930.0
9	3930.0
10	4304.0
11	4678.0
12	4678.0
13	4678.0
14	5287.0
15	5896.0
16	5896.0
17	5896.0
18	9286.0
19	12676.0
20	12676.0
21	12676.0
22	20326.0
23	27976.0
24	27976.0
25	27976.0
26	41218.0
27	54460.0
28	54460.0
29	54460.0
30	66820.0
31	79180.0
32	79180.0
33	79180.0
34	97797.5
35	116415.0
36	116415.0
37	116415.0
38	131487.5
39	146560.0
40	146560.0
41	146560.0
42	166341.0
43	186122.0
44	186122.0
45	186122.0
46	210948.5
47	235775.0
48	235775.0
49	235775.0
50	238972.5
51	242170.0
52	242170.0
53	242170.0
54	225078.5
55	207987.0
56	207987.0
57	207987.0
58	192498.5
59	177010.0
60	177010.0
61	177010.0
62	159835.0
63	142660.0
64	142660.0
65	142660.0
66	121675.0
67	100690.0
68	100690.0
69	100690.0
70	77365.5
71	54041.0
72	54041.0
73	54041.0
74	43421.0
75	32801.0
76	32801.0
77	32801.0
78	26607.0
79	20413.0
80	20413.0
81	20413.0
82	14625.0
83	8837.0
84	8837.0
85	8837.0
86	6551.0
87	4265.0
88	4265.0
89	4265.0
90	2852.5
91	1440.0
92	1440.0
93	1440.0
94	867.0
95	294.0
96	294.0
97	294.0
98	560.0
99	826.0
100	826.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00931275606734086
2	0.003960597407949561
3	0.004977507553233908
4	0.014718436313326073
5	0.035591855084952134
6	0.04688490985626778
7	0.06973862733186863
8	0.09264586639406339
9	0.10153044976865294
10	0.12465177517722335
11	0.12481233993700509
12	0.13792512865251377
13	0.12615037960185294
14	0.1293616747974877
15	0.11855031430551728
16	0.11812214161276598
17	0.12764898402648248
18	0.13524904932281812
19	0.13706878326701116
20	0.1286123725851729
21	0.13621243788150855
22	0.15826333155820071
23	0.14611393140138246
24	0.1400659921162703
25	0.13503496297644246
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1868405.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.919120360892855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.2200091597575	40.847323017576194
2	12.143056021377083	12.366274622031288
3	3.2903139821650553	5.026196810489734
4	1.3359971531267942	2.721111993674938
5	0.7175620466357199	1.8268814109526417
6	0.42919047300511143	1.3112400811617484
7	0.2925277805401588	1.0426680086360438
8	0.22314662965814697	0.9089944074952614
9	0.15776609764669403	0.7229979823446351
>10	0.9618487129379883	9.038849767368182
>50	0.10515800214292796	3.7938543570208414
>100	0.10346983804443387	11.171893744750111
>500	0.014675239744829862	5.1035045053747865
>1k	0.005278863217564699	4.118209291123587
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3419	0.1829903045645885	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2377	0.12722081133373117	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2313	0.12379542979172074	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2233	0.11951370286420769	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2155	0.11533901910988249	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2111	0.11298406929975031	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2082	0.11143194328852686	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2006	0.10736430270738946	No Hit
GAACTACGACGGTATCTGATCGTCT	1987	0.10634739256210511	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1974	0.10565161193638424	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1957	0.10474174496428772	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1903	0.10185157928821642	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1884	0.10083466914293207	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.352158659391299E-5
2	0.0	0.0	0.0	0.0	5.352158659391299E-5
3	0.0	0.0	0.0	0.0	5.352158659391299E-5
4	0.0	0.0	0.0	0.0	5.352158659391299E-5
5	0.0	0.0	0.0	0.0	5.352158659391299E-5
6	0.0	0.0	0.0	0.0	5.352158659391299E-5
7	0.0	0.0	0.0	1.0704317318782598E-4	5.352158659391299E-5
8	0.0	0.0	0.0	2.6760793296956494E-4	5.352158659391299E-5
9	0.0	0.0	0.0	3.2112951956347793E-4	1.0704317318782598E-4
10	0.0	0.0	0.0	3.746511061573909E-4	1.0704317318782598E-4
11	0.0	0.0	0.0	3.746511061573909E-4	2.1408634637565195E-4
12	0.0	0.0	0.0	3.746511061573909E-4	4.281726927513039E-4
13	0.0	0.0	0.0	4.281726927513039E-4	4.816942793452169E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAATAC	115	2.5465852E-11	14.036844	3
CGCGTAA	175	0.0	13.571926	10
ACGCTTA	120	5.0931703E-11	13.462797	17
GCGTAAC	185	0.0	13.351484	11
CGCCAGT	325	0.0	13.158562	18
GTCCTAA	260	0.0	13.144462	1
TGAACGG	120	7.421477E-10	12.665434	5
ATCGCCA	340	0.0	12.577363	16
AATCGTA	100	1.4398029E-7	12.352109	13
TCGACGC	125	1.4078978E-9	12.162077	14
TCGCCAG	360	0.0	12.142915	17
CGCATCG	345	0.0	12.1180105	13
TCGCGTA	200	0.0	11.875435	9
CGCAAGA	400	0.0	11.866847	2
CAAGACG	405	0.0	11.725996	4
CGCTTAC	130	2.6011548E-9	11.696498	18
CGTTCAA	65	8.01326E-4	11.694304	14
CTCGACG	130	2.6084308E-9	11.694304	13
CATCGCC	375	0.0	11.655636	15
AAGACGG	410	0.0	11.584238	5
>>END_MODULE