FastQCFastQC Report
Thu 2 Feb 2017
SRR4062578_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062578_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868405
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC23540.1259898148420712No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT23300.12470529676381727No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA21650.11587423497582162No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT21490.11501788959031901No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA19990.10698965160123207No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA19680.10533048241682076No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG19170.1026008815005312No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG500.002580996121.9999479
GTATCAA37500.018.598781
ACCGTCG4750.018.0631148
AAACGCT4850.017.6906829
TAGGACG4400.017.4999584
ACGCTTC5050.017.425731
GTAAACG4950.017.33329227
CGTCGTA4700.017.31910710
TAGTACG2700.017.1115282
TAAACGC5150.017.08733728
CGCAATA6850.017.02185636
TATACTG3250.016.9230375
TTAGTAC1307.322251E-716.9230353
ATACGAA6650.016.8721440
ATACCGT4600.016.739096
CGCTTCG5300.016.60373332
GTATTAG5200.016.5012841
TGCGTAG2550.016.39211713
TGCACCG1351.0862495E-616.2962575
CCGTCGT4750.016.2104859
Produced by FastQC (version 0.11.5)