##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062578_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1868405
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.309779196694507	32.0	32.0	32.0	32.0	32.0
2	31.436094957998936	32.0	32.0	32.0	32.0	32.0
3	31.512190344170563	32.0	32.0	32.0	32.0	32.0
4	31.61050575223252	32.0	32.0	32.0	32.0	32.0
5	31.55124504590814	32.0	32.0	32.0	32.0	32.0
6	35.1431343846757	36.0	36.0	36.0	36.0	36.0
7	35.15207677136381	36.0	36.0	36.0	36.0	36.0
8	35.10016243801531	36.0	36.0	36.0	36.0	36.0
9	35.224276856463135	36.0	36.0	36.0	36.0	36.0
10	35.09824529478352	36.0	36.0	36.0	36.0	36.0
11	35.231140999943804	36.0	36.0	36.0	36.0	36.0
12	35.14413470312914	36.0	36.0	36.0	36.0	36.0
13	35.18394191837423	36.0	36.0	36.0	36.0	36.0
14	35.141528737077884	36.0	36.0	36.0	36.0	36.0
15	35.103915906883145	36.0	36.0	36.0	36.0	36.0
16	35.118725865109546	36.0	36.0	36.0	36.0	36.0
17	35.0876421332634	36.0	36.0	36.0	36.0	36.0
18	35.108715722768885	36.0	36.0	36.0	36.0	36.0
19	35.088410703246886	36.0	36.0	36.0	36.0	36.0
20	35.079533077678555	36.0	36.0	36.0	36.0	36.0
21	35.082338679247805	36.0	36.0	36.0	36.0	36.0
22	35.059117803688174	36.0	36.0	36.0	36.0	36.0
23	35.02582255988396	36.0	36.0	36.0	36.0	36.0
24	34.998870159307	36.0	36.0	36.0	36.0	36.0
25	34.971776996957296	36.0	36.0	36.0	32.0	36.0
26	34.93118462003687	36.0	36.0	36.0	32.0	36.0
27	34.92076236147945	36.0	36.0	36.0	32.0	36.0
28	34.899754603525466	36.0	36.0	36.0	32.0	36.0
29	34.878835691405236	36.0	36.0	36.0	32.0	36.0
30	34.86135286514433	36.0	36.0	36.0	32.0	36.0
31	34.86522194063921	36.0	36.0	36.0	32.0	36.0
32	34.83050409306333	36.0	36.0	36.0	32.0	36.0
33	34.809211065052814	36.0	36.0	36.0	32.0	36.0
34	34.79620424907876	36.0	36.0	36.0	32.0	36.0
35	34.7701365603282	36.0	36.0	36.0	32.0	36.0
36	34.743805545371586	36.0	36.0	36.0	32.0	36.0
37	34.73551826290338	36.0	36.0	36.0	32.0	36.0
38	34.70655826761328	36.0	36.0	36.0	32.0	36.0
39	34.70142019530027	36.0	36.0	36.0	32.0	36.0
40	34.693602832362366	36.0	36.0	36.0	32.0	36.0
41	34.647757846933615	36.0	36.0	36.0	32.0	36.0
42	34.628340215317344	36.0	36.0	36.0	32.0	36.0
43	34.62069198059307	36.0	36.0	36.0	32.0	36.0
44	34.58980841948079	36.0	36.0	36.0	32.0	36.0
45	34.55388794185415	36.0	36.0	36.0	32.0	36.0
46	34.549766244470554	36.0	36.0	36.0	32.0	36.0
47	34.52400095268424	36.0	36.0	36.0	32.0	36.0
48	34.48914983635775	36.0	36.0	36.0	32.0	36.0
49	34.47370457689848	36.0	36.0	36.0	32.0	36.0
50	33.95890237930213	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	4.0
20	14.0
21	57.0
22	175.0
23	472.0
24	1223.0
25	2867.0
26	5796.0
27	10715.0
28	18331.0
29	27723.0
30	40507.0
31	57447.0
32	85473.0
33	141337.0
34	315256.0
35	1161005.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.67276301125092	18.078982331913362	11.621346448878388	26.626908207957328
2	16.738977077974283	20.39137541788567	36.20525023898297	26.664397265157085
3	18.71765942551793	23.718954654152547	28.447770172918375	29.115615747411148
4	12.675872404196104	15.439895097409547	35.600888460715055	36.2833440376793
5	14.707946082353665	36.328151551724595	33.18857528212567	15.775327083796073
6	34.68001430103683	35.14265712407862	16.380681611392873	13.79664696349168
7	30.595968856825856	30.224314561687173	20.523677172430144	18.656039409056827
8	28.434214441844485	32.428372022324936	19.406991207469048	19.730422328361534
9	27.69570837157897	14.044010800656176	18.55459603244479	39.705684795320074
10	16.235505685330537	26.220332315531163	31.06965566887265	26.47450633026566
11	37.92346948332936	21.03462579044693	21.873094966027175	19.16880976019653
12	24.83406970116222	23.71193611663424	28.13137408645342	23.322620095750118
13	29.600595160042925	19.00096606463802	25.20213765216856	26.196301123150494
14	23.62480297365935	19.570114616477692	24.630741193691946	32.17434121617101
15	25.385770215772276	27.096962382352864	21.922227782520384	25.595039619354477
16	26.249822709744407	25.569723908895554	23.45439024194433	24.726063139415704
17	24.41537032923804	25.629132870014796	24.9960260221954	24.959470778551758
18	25.344183942988806	24.403006842734847	26.086635392219566	24.16617382205678
19	25.88154067239169	24.714020782432076	25.18993473042515	24.214503814751083
20	25.96647943031623	23.968625645938648	24.930301513858076	25.13459340988704
21	27.090111619268843	23.943845151345666	24.16660199474953	24.79944123463596
22	26.125973758366094	24.24436886007049	24.72766878701352	24.9019885945499
23	24.43463810041185	23.96605660978214	25.500199367910064	26.099105921895948
24	24.886788463957227	24.763153598925285	25.128224341082365	25.221833596035122
25	24.902791418348805	24.264439455043206	25.32149079027299	25.511278336335003
26	24.605211396886652	25.16868666054737	25.628918783668425	24.59718315889756
27	25.67104027231783	24.50865845467123	24.734144898991385	25.086156374019552
28	24.56378568886296	24.581661898785327	25.656696487110665	25.197855925241047
29	24.633470794608236	24.81025259512793	25.37624337335856	25.180033236905274
30	24.572028013198423	24.89631530637094	25.662048645770053	24.86960803466058
31	25.234357647298093	24.6663330487769	24.59177747865158	25.50753182527343
32	25.043820799023763	24.651935741983134	24.70754467045421	25.596698788538884
33	24.649045576307063	24.118700174747982	25.367786962676718	25.864467286268233
34	25.230129441957178	24.490835766335454	25.499075414591587	24.779959377115777
35	25.945713054717796	24.28622274078693	25.26299169612584	24.505072508369437
36	24.501486562067644	24.90803653383501	25.205509512123975	25.38496739197337
37	25.76149175366155	24.693522014766607	24.683138826967387	24.86184740460446
38	24.680355704464503	24.3603501382195	25.475525916490266	25.483768240825732
39	25.31265972848499	24.30559755513392	24.720336329650156	25.661406386730928
40	25.565067530861885	24.422542221841624	25.44426930991942	24.568120937377067
41	24.423238002467347	24.89508430987928	25.78734267998641	24.894335007666967
42	25.831016294647043	24.901667465030332	25.1973742309617	24.069942009360926
43	24.97162017870858	23.843599219655268	25.583639521409975	25.60114108022618
44	24.478525801418858	24.520540246895077	25.217177218001453	25.783756733684616
45	24.49843583163179	24.87629823298482	25.327110556865346	25.29815537851804
46	24.351866272961452	24.353204314058583	25.4296316158773	25.86529779710266
47	24.648885011547282	24.201123418102608	25.86098838313963	25.28900318721048
48	25.33176693489902	24.983180841412864	24.86398826806822	24.821063955619902
49	24.297462273971647	24.96096938297639	25.35997281103401	25.381595532017954
50	24.111273578947596	25.36582024016219	25.114108083690677	25.408798097199536
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	48.0
1	55.0
2	62.0
3	167.0
4	272.0
5	315.5
6	359.0
7	333.5
8	308.0
9	421.0
10	534.0
11	769.0
12	1004.0
13	1740.0
14	2476.0
15	3310.0
16	4144.0
17	4691.0
18	5238.0
19	5623.0
20	6008.0
21	6928.5
22	7849.0
23	8636.5
24	9424.0
25	11168.5
26	12913.0
27	15890.0
28	18867.0
29	21842.5
30	24818.0
31	27516.0
32	30214.0
33	34974.5
34	39735.0
35	45660.0
36	51585.0
37	61138.5
38	70692.0
39	76230.5
40	81769.0
41	88286.5
42	94804.0
43	97067.0
44	99330.0
45	108855.0
46	118380.0
47	125741.0
48	133102.0
49	136448.0
50	139794.0
51	133260.0
52	126726.0
53	123990.0
54	121254.0
55	123521.0
56	125788.0
57	123877.5
58	121967.0
59	112499.0
60	103031.0
61	91007.0
62	78983.0
63	70929.5
64	62876.0
65	53960.5
66	45045.0
67	41652.5
68	38260.0
69	35933.5
70	33607.0
71	26367.0
72	19127.0
73	17067.0
74	15007.0
75	11109.0
76	7211.0
77	6440.0
78	5669.0
79	4905.0
80	4141.0
81	3198.0
82	2255.0
83	2019.0
84	1783.0
85	1482.0
86	1181.0
87	849.5
88	518.0
89	345.5
90	173.0
91	108.0
92	43.0
93	28.0
94	13.0
95	10.0
96	7.0
97	7.0
98	7.0
99	5.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01557478169882868
2	0.003800032648167822
3	4.816942793452169E-4
4	2.6760793296956494E-4
5	0.0
6	4.816942793452169E-4
7	1.0704317318782599E-4
8	5.3521586593912995E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.0704317318782599E-4
47	0.0
48	0.0
49	0.0
50	5.3521586593912995E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1868405.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.87853690494667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.58834412958356	43.41982073294311
2	12.057586787818295	12.992902694641309
3	3.2535445289535483	5.258886569253333
4	1.301292972668045	2.8044704580817212
5	0.6823488164776257	1.838197794531822
6	0.3959140684608815	1.2798762449254315
7	0.29137640469636983	1.0989254061564826
8	0.20050570749998034	0.8642363328952107
9	0.15736269964403996	0.7630624818210102
>10	0.8751486146671632	8.641427858165498
>50	0.09250008818676209	3.530298046590661
>100	0.08829494292506616	10.05273733070754
>500	0.011785241350619143	4.315169868779846
>1k	0.0039949970680064895	3.1399881805070593
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2354	0.1259898148420712	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2330	0.12470529676381727	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2165	0.11587423497582162	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2149	0.11501788959031901	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1999	0.10698965160123207	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1968	0.10533048241682076	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1917	0.1026008815005312	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.0704317318782598E-4	0.0
5	0.0	0.0	0.0	1.0704317318782598E-4	0.0
6	0.0	0.0	0.0	1.0704317318782598E-4	0.0
7	0.0	0.0	0.0	1.0704317318782598E-4	0.0
8	0.0	0.0	0.0	1.0704317318782598E-4	0.0
9	0.0	0.0	0.0	1.0704317318782598E-4	5.352158659391299E-5
10	0.0	0.0	0.0	1.0704317318782598E-4	5.352158659391299E-5
11	0.0	0.0	0.0	1.0704317318782598E-4	5.352158659391299E-5
12	0.0	0.0	0.0	1.0704317318782598E-4	5.352158659391299E-5
13	0.0	0.0	0.0	1.0704317318782598E-4	5.352158659391299E-5
14	0.0	0.0	0.0	1.0704317318782598E-4	1.0704317318782598E-4
15	0.0	0.0	0.0	1.6056475978173896E-4	1.6056475978173896E-4
16	0.0	0.0	0.0	1.6056475978173896E-4	1.6056475978173896E-4
17	0.0	0.0	0.0	2.1408634637565195E-4	1.6056475978173896E-4
18	0.0	0.0	0.0	3.2112951956347793E-4	2.1408634637565195E-4
19	0.0	0.0	0.0	3.746511061573909E-4	2.1408634637565195E-4
20	0.0	0.0	0.0	5.887374525330429E-4	2.6760793296956494E-4
21	0.0	0.0	0.0	5.887374525330429E-4	3.2112951956347793E-4
22	0.0	0.0	0.0	9.633885586904338E-4	3.2112951956347793E-4
23	0.0	0.0	0.0	0.0016591691844113027	3.2112951956347793E-4
24	0.0	0.0	0.0	0.0027296009162895624	3.2112951956347793E-4
25	0.0	0.0	0.0	0.0033183383688226054	3.2112951956347793E-4
26	0.0	0.0	0.0	0.004174683754325214	5.352158659391299E-4
27	0.0	0.0	0.0	0.006315547218081733	5.887374525330429E-4
28	0.0	0.0	0.0	0.014290263620574768	5.887374525330429E-4
29	0.0	0.0	0.0	0.028419962481367796	5.887374525330429E-4
30	0.0	0.0	0.0	0.05951600429243124	5.887374525330429E-4
31	0.0	0.0	0.0	0.12117287204861901	5.887374525330429E-4
32	0.0	0.0	0.0	0.19712000342538155	5.887374525330429E-4
33	0.0	0.0	0.0	0.2715685303775145	5.887374525330429E-4
34	0.0	0.0	0.0	0.3553298133969883	6.957806257208689E-4
35	0.0	0.0	0.0	0.44915315469611783	6.957806257208689E-4
36	0.0	0.0	0.0	0.5833317722870577	6.957806257208689E-4
37	0.0	0.0	0.0	0.7608093534324731	6.957806257208689E-4
38	0.0	0.0	0.0	1.0070621733510667	6.957806257208689E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTACG	50	0.0025809961	21.999947	9
GTATCAA	3750	0.0	18.59878	1
ACCGTCG	475	0.0	18.063114	8
AAACGCT	485	0.0	17.69068	29
TAGGACG	440	0.0	17.499958	4
ACGCTTC	505	0.0	17.4257	31
GTAAACG	495	0.0	17.333292	27
CGTCGTA	470	0.0	17.319107	10
TAGTACG	270	0.0	17.111528	2
TAAACGC	515	0.0	17.087337	28
CGCAATA	685	0.0	17.021856	36
TATACTG	325	0.0	16.923037	5
TTAGTAC	130	7.322251E-7	16.923035	3
ATACGAA	665	0.0	16.87214	40
ATACCGT	460	0.0	16.73909	6
CGCTTCG	530	0.0	16.603733	32
GTATTAG	520	0.0	16.501284	1
TGCGTAG	255	0.0	16.392117	13
TGCACCG	135	1.0862495E-6	16.296257	5
CCGTCGT	475	0.0	16.210485	9
>>END_MODULE