FastQCFastQC Report
Thu 2 Feb 2017
SRR4062577_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062577_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1594538
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGC22660.1421101284509996No Hit
GTCTTGCGCCGGTCCAAGAATTTCA22610.14179655799987206No Hit
GTATCAACGCAGAGTACTTTTTTTT20050.12574175090214218No Hit
GTACATGGAAGCAGTGGTATCAACG19970.12524003818033813No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG18620.11677363599989464No Hit
GAACTACGACGGTATCTGATCGTCT18270.11457864284200188No Hit
GAATAGGACCGCGGTTCTATTTTGT18130.11370064557884478No Hit
TCCATGTACTCTGCGTTGATACCAC17200.10786823518787261No Hit
GGGTAGGCACACGCTGAGCCAGTCA16850.10567324202997984No Hit
GTCCTATTCCATTATTCCTAGCTGC16630.10429353204501868No Hit
GATTAAGAGGGACGGCCGGGGGCAT16300.10222396706757693No Hit
GTATCTGATCGTCTTCGAACCTCCG16220.10172225434577288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA902.2846507E-914.77948815
CAAGACG3950.013.7065744
AAGACGG3950.013.4669525
GACGCTT500.001497205513.30279316
ACGGTAT4050.012.9027279
TAAATCC2150.012.8118164
AGGCCCG3550.012.57891910
CGCAAGA4250.012.5120232
CGGTCCA4200.012.441915510
GTCGCGT1700.012.2951548
CGCCGGT4050.012.1985597
CGCATCG2350.012.12830113
TCGCGTA1650.012.0923089
AAGATAG550.003070896512.0896515
TCGGCGT2450.012.02104413
CGGTCGG2550.011.92294910
GCGCAAG4300.011.9226271
ATCGCCA2400.011.87749216
CGGTTCT3700.011.81180612
CGGCGTC2500.011.780622514
Produced by FastQC (version 0.11.5)