##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062577_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1594538
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.137623562436268	32.0	32.0	32.0	32.0	32.0
2	30.71906345286221	32.0	32.0	32.0	32.0	32.0
3	30.704693773368838	32.0	32.0	32.0	32.0	32.0
4	30.791560314021993	32.0	32.0	32.0	32.0	32.0
5	30.53945970556989	32.0	32.0	32.0	32.0	32.0
6	34.200485030773805	36.0	36.0	36.0	32.0	36.0
7	34.08448026952007	36.0	36.0	36.0	32.0	36.0
8	34.071092065538735	36.0	36.0	36.0	32.0	36.0
9	34.27781714828998	36.0	36.0	36.0	32.0	36.0
10	33.864871831213804	36.0	36.0	36.0	32.0	36.0
11	34.23706365103873	36.0	36.0	36.0	32.0	36.0
12	33.97848028708002	36.0	36.0	36.0	32.0	36.0
13	34.12454328463793	36.0	36.0	36.0	32.0	36.0
14	34.01138887878495	36.0	36.0	36.0	32.0	36.0
15	33.9306413519151	36.0	36.0	36.0	32.0	36.0
16	33.932847633609235	36.0	36.0	36.0	32.0	36.0
17	33.85131931631607	36.0	36.0	36.0	32.0	36.0
18	33.86940856850072	36.0	36.0	36.0	32.0	36.0
19	33.863788758875614	36.0	36.0	36.0	32.0	36.0
20	33.85498495488976	36.0	36.0	36.0	32.0	36.0
21	33.84727676606014	36.0	36.0	36.0	32.0	36.0
22	33.79666461382545	36.0	36.0	36.0	32.0	36.0
23	33.744992593465945	36.0	36.0	36.0	32.0	36.0
24	33.72297806637408	36.0	36.0	36.0	27.0	36.0
25	33.249579502025036	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	12.0
4	64.0
5	147.0
6	255.0
7	82.0
8	229.0
9	287.0
10	165.0
11	66.0
12	105.0
13	101.0
14	236.0
15	331.0
16	513.0
17	706.0
18	985.0
19	1365.0
20	2234.0
21	3460.0
22	5233.0
23	7677.0
24	11071.0
25	15538.0
26	21585.0
27	27535.0
28	36943.0
29	48487.0
30	61741.0
31	83926.0
32	118383.0
33	169565.0
34	362036.0
35	613473.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.50846676296137	17.161384360745128	11.00920653457609	26.320942341717412
2	17.15902370250233	19.297974942190933	36.267343504719115	27.275657850587624
3	19.63386954446618	22.67584177454547	27.840101250643635	29.85018743034471
4	12.871441143024168	14.622871397236526	34.86056863934386	37.645118820395446
5	15.056562249842534	35.92505878308352	33.248640523564674	15.769738443509269
6	35.63633648575137	34.33354059441474	16.143086692646456	13.887036227187425
7	31.128272808228076	29.767407125702317	20.235385520037706	18.868934546031902
8	28.65048927609734	32.59959702982105	19.061556519392155	19.688357174689454
9	26.919332270795287	14.451919316577538	18.583388625904796	40.04535978672237
10	16.24992544177709	26.23959716584574	31.015467299548256	26.495010092828913
11	38.28305215638754	20.820299991837814	21.371436105756857	19.525211746017792
12	24.72349956954285	23.42723013539149	27.99101785690738	23.858252438158285
13	29.394380231292917	18.839969134155293	25.19735367448589	26.5682969600659
14	23.404430324111864	19.503911694900356	24.29206484748785	32.79959313349993
15	25.40301594107997	27.05959242919558	21.709903861306138	25.827487768418315
16	26.465641450621064	25.367850405095314	23.249300775033195	24.917207369250423
17	24.37646727893416	25.890287877065965	24.743714756266094	24.989530087733783
18	25.50766353896069	24.283707609411625	25.766557972447806	24.442070879179877
19	25.65091313706726	24.96646849168494	24.79780626072192	24.58481211052588
20	25.71725107021005	24.063369996948538	24.64063671376135	25.578742219080063
21	26.617487121137913	24.14362788314458	24.08447679544273	25.15440820027478
22	25.739511742314097	24.253359903983903	24.481718441961696	25.525409911740304
23	24.25412782504503	24.04412558937529	25.240460095807236	26.46128648977244
24	24.799919119914147	25.014678354090254	24.7109384664558	25.474464059539798
25	25.07031216150793	24.430893772907574	24.68834397356175	25.810450092022748
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	182.0
1	182.0
2	387.0
3	592.0
4	592.0
5	592.0
6	1490.0
7	2388.0
8	2388.0
9	2388.0
10	2707.0
11	3026.0
12	3026.0
13	3026.0
14	3599.0
15	4172.0
16	4172.0
17	4172.0
18	6672.5
19	9173.0
20	9173.0
21	9173.0
22	14451.5
23	19730.0
24	19730.0
25	19730.0
26	29658.5
27	39587.0
28	39587.0
29	39587.0
30	49663.5
31	59740.0
32	59740.0
33	59740.0
34	76265.5
35	92791.0
36	92791.0
37	92791.0
38	107112.0
39	121433.0
40	121433.0
41	121433.0
42	140003.0
43	158573.0
44	158573.0
45	158573.0
46	183280.0
47	207987.0
48	207987.0
49	207987.0
50	211814.0
51	215641.0
52	215641.0
53	215641.0
54	203315.5
55	190990.0
56	190990.0
57	190990.0
58	178019.0
59	165048.0
60	165048.0
61	165048.0
62	145956.0
63	126864.0
64	126864.0
65	126864.0
66	104689.0
67	82514.0
68	82514.0
69	82514.0
70	62791.0
71	43068.0
72	43068.0
73	43068.0
74	33732.5
75	24397.0
76	24397.0
77	24397.0
78	20005.0
79	15613.0
80	15613.0
81	15613.0
82	10889.0
83	6165.0
84	6165.0
85	6165.0
86	4575.5
87	2986.0
88	2986.0
89	2986.0
90	1995.0
91	1004.0
92	1004.0
93	1004.0
94	598.5
95	193.0
96	193.0
97	193.0
98	437.0
99	681.0
100	681.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00796468945863943
2	0.004076415864657976
3	0.004954413127815079
4	0.015114095744347264
5	0.03399103690222497
6	0.04609485631574788
7	0.06691593427061632
8	0.08579287542849402
9	0.09388299306758446
10	0.11432778648109984
11	0.1146413569322274
12	0.12825031451116248
13	0.11533121192470798
14	0.11777706144350276
15	0.1088089465412552
16	0.10680209565403898
17	0.11783977553372826
18	0.12524003818033813
19	0.1258044649923677
20	0.11664820781944363
21	0.1258044649923677
22	0.14455597796979439
23	0.1363404321502529
24	0.12900288359386855
25	0.12524003818033813
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1594538.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.857895559479104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.41927936086365	42.507938694251045
2	11.603236675593365	12.266453447008633
3	3.264974058521628	5.177389733692346
4	1.485376995667591	3.1405560841380145
5	0.8167334822232314	2.1585406551642623
6	0.4957291738859837	1.5721920539431309
7	0.34669634984054565	1.2827947615506932
8	0.24576524547720227	1.0392506942066961
9	0.17320102898249506	0.8239537710675933
>10	0.9587928402215097	9.04439772270121
>50	0.08515988284120865	3.151348205555829
>100	0.08843621111007069	10.087061875790711
>500	0.012107906190694706	4.36171486443108
>1k	0.0045107885808470475	3.386407436498738
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGC	2266	0.1421101284509996	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2261	0.14179655799987206	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2005	0.12574175090214218	No Hit
GTACATGGAAGCAGTGGTATCAACG	1997	0.12524003818033813	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1862	0.11677363599989464	No Hit
GAACTACGACGGTATCTGATCGTCT	1827	0.11457864284200188	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1813	0.11370064557884478	No Hit
TCCATGTACTCTGCGTTGATACCAC	1720	0.10786823518787261	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1685	0.10567324202997984	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1663	0.10429353204501868	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1630	0.10222396706757693	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1622	0.10172225434577288	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.5085636090202933E-4
2	0.0	0.0	0.0	0.0	2.5085636090202933E-4
3	0.0	0.0	0.0	0.0	2.5085636090202933E-4
4	0.0	0.0	0.0	0.0	2.5085636090202933E-4
5	0.0	0.0	0.0	0.0	3.1357045112753663E-4
6	0.0	0.0	0.0	0.0	3.1357045112753663E-4
7	0.0	0.0	0.0	6.271409022550733E-5	3.1357045112753663E-4
8	0.0	0.0	0.0	6.271409022550733E-5	3.1357045112753663E-4
9	0.0	0.0	0.0	6.271409022550733E-5	3.1357045112753663E-4
10	6.271409022550733E-5	0.0	0.0	6.271409022550733E-5	3.1357045112753663E-4
11	6.271409022550733E-5	0.0	0.0	6.271409022550733E-5	3.1357045112753663E-4
12	6.271409022550733E-5	0.0	0.0	6.271409022550733E-5	4.389986315785513E-4
13	6.271409022550733E-5	0.0	0.0	6.271409022550733E-5	6.271409022550733E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTA	90	2.2846507E-9	14.779488	15
CAAGACG	395	0.0	13.706574	4
AAGACGG	395	0.0	13.466952	5
GACGCTT	50	0.0014972055	13.302793	16
ACGGTAT	405	0.0	12.902727	9
TAAATCC	215	0.0	12.811816	4
AGGCCCG	355	0.0	12.578919	10
CGCAAGA	425	0.0	12.512023	2
CGGTCCA	420	0.0	12.4419155	10
GTCGCGT	170	0.0	12.295154	8
CGCCGGT	405	0.0	12.198559	7
CGCATCG	235	0.0	12.128301	13
TCGCGTA	165	0.0	12.092308	9
AAGATAG	55	0.0030708965	12.089651	5
TCGGCGT	245	0.0	12.021044	13
CGGTCGG	255	0.0	11.922949	10
GCGCAAG	430	0.0	11.922627	1
ATCGCCA	240	0.0	11.877492	16
CGGTTCT	370	0.0	11.811806	12
CGGCGTC	250	0.0	11.7806225	14
>>END_MODULE