FastQCFastQC Report
Thu 2 Feb 2017
SRR4062577_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062577_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1594538
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC22380.1403541339246854No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT21420.13433358126303668No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA18930.11871777279688536No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT18550.11633463736831608No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA17300.10849537609012767No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17120.10736652246606854No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17090.10717838019539201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTTA5750.018.36511242
CGCAATA6300.017.80942236
TCTAGCG6600.017.66656528
CTAGCGG6550.017.12967329
GTATAGT902.2144338E-417.1126231
CATACGG902.215495E-417.111555
CGGTCCA6950.017.09342810
TTAGAAC1551.4395482E-817.0326963
TAACAGT2350.016.8514984
ATACGAA6850.016.70063440
TAGCGGC6600.016.6665730
ATACCGT5150.016.6606226
TAGGACG3450.016.5801354
CGTTATT6500.016.24606144
TACGAAT7050.016.22685641
GTAATAC953.3464967E-416.2109433
CCTAACC1501.8381252E-716.1337473
TACACCG1501.8381252E-716.1337475
CGGCGTT6550.016.12204641
TACGCTA5050.016.1187219
Produced by FastQC (version 0.11.5)