FastQCFastQC Report
Thu 2 Feb 2017
SRR4062576_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062576_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences899763
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG85110.945915757816225No Hit
TCCATGTACTCTGCGTTGATACCAC69990.7778715061632897No Hit
GAGTACATGGAAGCAGTGGTATCAA38890.4322249303427681No Hit
CATGTACTCTGCGTTGATACCACTG31780.3532041215297806No Hit
GCTTCCATGTACTCTGCGTTGATAC23090.25662313298057376No Hit
GTATCAACGCAGAGTACTTTTTTTT22500.2500658506740108No Hit
GCGTTGATACCACTGCTTCCATGTA19470.21639031611657733No Hit
CATGGAAGCAGTGGTATCAACGCAG18460.20516513793076618No Hit
CCCATGTACTCTGCGTTGATACCAC17790.19771873259958453No Hit
GTACATGGGAAGCAGTGGTATCAAC16320.18138109702221586No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15790.17549065698411692No Hit
TATCAACGCAGAGTACTTTTTTTTT15220.16915565543370864No Hit
GTATCAACGCAGAGTACATGGAAGC15050.1672662690063939No Hit
ACGCAGAGTACATGGAAGCAGTGGT13420.1491503873797878No Hit
ACTCTGCGTTGATACCACTGCTTCC12850.14281538582937953No Hit
CAGTGGTATCAACGCAGAGTACATG12460.1384809110843633No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11280.12536634647123743No Hit
GTCTTGCGCCGGTCCAAGAATTTCA9770.10858414938155936No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9150.1016934459407644No Hit
GCGCAAGACGGACCAGAGCGAAAGC9000.10002634026960433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT402.7696782E-416.6169722
GCGTTAT508.747523E-515.1918151
TCGGCGT851.7142156E-814.52885813
ATCGCCA1600.014.25342216
TCCCGTT707.2489147E-613.57242211
GCACGCG500.0014959813.30319319
GCCGTTA500.001498491213.30023515
AATACTG500.00150100613.2972765
TCCAACG1153.7471182E-1013.22056718
CCCGTTT801.9925956E-613.0649112
CCAACGT604.080768E-412.66970919
ATAACGA604.0826842E-412.669003512
GTCGGCG905.384409E-712.669003512
ACCGTCG1352.7284841E-1112.66838
TTGCGCG1001.4352918E-712.35296518
GCGGTCG1001.4384023E-712.3509049
TCGCCAG1850.012.32728417
CGCATCG1850.012.32385513
ATTTGCG853.927533E-612.2970716
CGAACGA701.0874731E-412.21721916
Produced by FastQC (version 0.11.5)