##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062576_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	899763
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16047892611721	32.0	32.0	32.0	32.0	32.0
2	30.852663423590435	32.0	32.0	32.0	32.0	32.0
3	30.845884971931497	32.0	32.0	32.0	32.0	32.0
4	30.90437593010604	32.0	32.0	32.0	32.0	32.0
5	30.734367827972477	32.0	32.0	32.0	32.0	32.0
6	34.38662292181385	36.0	36.0	36.0	32.0	36.0
7	34.261212119191384	36.0	36.0	36.0	32.0	36.0
8	34.248104223000944	36.0	36.0	36.0	32.0	36.0
9	34.390378355189085	36.0	36.0	36.0	32.0	36.0
10	34.098804907514534	36.0	36.0	36.0	32.0	36.0
11	34.39400153151441	36.0	36.0	36.0	32.0	36.0
12	34.182489166591644	36.0	36.0	36.0	32.0	36.0
13	34.30435459115345	36.0	36.0	36.0	32.0	36.0
14	34.198276657297534	36.0	36.0	36.0	32.0	36.0
15	34.148872536434595	36.0	36.0	36.0	32.0	36.0
16	34.133085045728706	36.0	36.0	36.0	32.0	36.0
17	34.05485555640763	36.0	36.0	36.0	32.0	36.0
18	34.07609670546577	36.0	36.0	36.0	32.0	36.0
19	34.06152620189983	36.0	36.0	36.0	32.0	36.0
20	34.049087370785415	36.0	36.0	36.0	32.0	36.0
21	33.98958170095903	36.0	36.0	36.0	32.0	36.0
22	33.947157195839345	36.0	36.0	36.0	32.0	36.0
23	33.86799079313108	36.0	36.0	36.0	32.0	36.0
24	33.84811555931951	36.0	36.0	36.0	32.0	36.0
25	33.34049633070042	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	35.0
5	81.0
6	151.0
7	39.0
8	128.0
9	135.0
10	120.0
11	49.0
12	58.0
13	53.0
14	204.0
15	228.0
16	319.0
17	480.0
18	579.0
19	876.0
20	1226.0
21	1707.0
22	2615.0
23	3774.0
24	5249.0
25	7301.0
26	10250.0
27	13372.0
28	17912.0
29	24301.0
30	32096.0
31	43940.0
32	63857.0
33	91734.0
34	202338.0
35	374550.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.481863298117695	16.80747364384597	11.415757562284996	25.294905495751337
2	15.709608151724504	19.987418531658175	37.71428507917879	26.58868823743854
3	20.049482666062026	23.999204187131618	27.925140239788643	28.026172907017717
4	12.544935140002	15.329857830441403	35.27250091704367	36.85270611251292
5	14.086669579593503	36.953701140377504	33.83164394694915	15.127985333079842
6	34.57699300038362	34.35649674481701	17.117648039940622	13.948862214858757
7	29.72764974037254	30.53472468590583	20.74283814126981	18.994787432451822
8	28.409610436705556	33.29202730647255	19.88618224411862	18.41218001270327
9	27.04522295544451	14.913830312684942	18.286417984167738	39.754528747702814
10	15.68687450900974	27.228734140875538	32.015457986811974	25.068933363302747
11	37.48424145923253	21.173358969822605	21.415370098996462	19.92702947194841
12	23.84060487083951	23.7465667748383	29.347119652070177	23.065708702252017
13	30.474723826987955	19.477986273689456	25.134970936184224	24.912318963138357
14	23.618472371672965	19.685954993039644	24.70364241911021	31.991930216177177
15	25.52223976465693	26.998919638217885	23.48212507496331	23.996715522161878
16	25.76097768303185	25.722147182062756	24.97824824372648	23.53862689117891
17	23.64001348664188	25.82191178712471	26.508706784604307	24.0293679416291
18	25.099292571724902	24.19589431197398	27.82907322110778	22.87573989519334
19	24.82633749618844	26.640417902873637	25.031215848970934	23.50202875196699
20	24.532582884332484	24.284547471685684	27.292908791069394	23.889960852912438
21	25.64318852804724	25.32391729884744	23.797892075418787	25.235002097686536
22	24.321992865133208	25.869179267702208	25.11973998363767	24.689087883526916
23	23.415351876914308	25.255649440843364	25.16149209345395	26.167506588788374
24	23.638813986175858	26.643355631651517	25.28108456408105	24.436745818091573
25	25.12583337135506	24.76861460323678	24.89069216978686	25.214859855621295
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	131.0
1	131.0
2	336.0
3	541.0
4	541.0
5	541.0
6	1481.0
7	2421.0
8	2421.0
9	2421.0
10	2412.0
11	2403.0
12	2403.0
13	2403.0
14	2450.0
15	2497.0
16	2497.0
17	2497.0
18	4005.5
19	5514.0
20	5514.0
21	5514.0
22	9259.5
23	13005.0
24	13005.0
25	13005.0
26	19763.0
27	26521.0
28	26521.0
29	26521.0
30	34135.0
31	41749.0
32	41749.0
33	41749.0
34	48946.0
35	56143.0
36	56143.0
37	56143.0
38	62689.5
39	69236.0
40	69236.0
41	69236.0
42	81899.5
43	94563.0
44	94563.0
45	94563.0
46	119635.0
47	144707.0
48	144707.0
49	144707.0
50	132714.5
51	120722.0
52	120722.0
53	120722.0
54	107914.5
55	95107.0
56	95107.0
57	95107.0
58	87257.5
59	79408.0
60	79408.0
61	79408.0
62	70514.0
63	61620.0
64	61620.0
65	61620.0
66	50638.5
67	39657.0
68	39657.0
69	39657.0
70	30082.0
71	20507.0
72	20507.0
73	20507.0
74	15842.0
75	11177.0
76	11177.0
77	11177.0
78	9140.5
79	7104.0
80	7104.0
81	7104.0
82	4920.0
83	2736.0
84	2736.0
85	2736.0
86	2027.0
87	1318.0
88	1318.0
89	1318.0
90	886.5
91	455.0
92	455.0
93	455.0
94	275.5
95	96.0
96	96.0
97	96.0
98	260.5
99	425.0
100	425.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007557545709258994
2	0.0030007902080881298
3	0.0060015804161762595
4	0.014781670284285973
5	0.03600948249705756
6	0.047568081817100726
7	0.0655728230656295
8	0.08691177565647844
9	0.0932467772068867
10	0.11880906416467447
11	0.11603055471274101
12	0.13236819029010974
13	0.11680853735928239
14	0.12247669664122664
15	0.11002897429656477
16	0.10947327240617807
17	0.1218098543727626
18	0.1284782770574029
19	0.13125678650933634
20	0.1223655562631493
21	0.12825599630124823
22	0.1509286334290252
23	0.14081485902398744
24	0.1322570499120324
25	0.12825599630124823
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	899763.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.02056123502818
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.116854118113	41.67723715609117
2	11.894046609281302	12.374699599407947
3	3.368611151111377	5.257111279901646
4	1.3849630841976988	2.8818622771903954
5	0.7733297858564847	2.0114524740009245
6	0.48877995580050015	1.5255964572704572
7	0.32123221419915066	1.1697476048587423
8	0.24967110550933347	1.0390424826212372
9	0.1737421959419794	0.8134349888797205
>10	1.018512396028033	9.669939470559632
>50	0.10213219889244414	3.7077053901032624
>100	0.09613506554575676	10.013806493779628
>500	0.008350261810624553	2.9635102135130036
>1k	0.003211639157932521	3.167337164718656
>5k	4.282185543910028E-4	1.727516947103645
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	8511	0.945915757816225	No Hit
TCCATGTACTCTGCGTTGATACCAC	6999	0.7778715061632897	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3889	0.4322249303427681	No Hit
CATGTACTCTGCGTTGATACCACTG	3178	0.3532041215297806	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2309	0.25662313298057376	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2250	0.2500658506740108	No Hit
GCGTTGATACCACTGCTTCCATGTA	1947	0.21639031611657733	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1846	0.20516513793076618	No Hit
CCCATGTACTCTGCGTTGATACCAC	1779	0.19771873259958453	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1632	0.18138109702221586	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1579	0.17549065698411692	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1522	0.16915565543370864	No Hit
GTATCAACGCAGAGTACATGGAAGC	1505	0.1672662690063939	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1342	0.1491503873797878	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1285	0.14281538582937953	No Hit
CAGTGGTATCAACGCAGAGTACATG	1246	0.1384809110843633	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1128	0.12536634647123743	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	977	0.10858414938155936	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	915	0.1016934459407644	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	900	0.10002634026960433	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	3.334211342320144E-4
13	0.0	0.0	0.0	1.1114037807733814E-4	6.668422684640288E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	40	2.7696782E-4	16.616972	2
GCGTTAT	50	8.747523E-5	15.191815	1
TCGGCGT	85	1.7142156E-8	14.528858	13
ATCGCCA	160	0.0	14.253422	16
TCCCGTT	70	7.2489147E-6	13.572422	11
GCACGCG	50	0.00149598	13.303193	19
GCCGTTA	50	0.0014984912	13.300235	15
AATACTG	50	0.001501006	13.297276	5
TCCAACG	115	3.7471182E-10	13.220567	18
CCCGTTT	80	1.9925956E-6	13.06491	12
CCAACGT	60	4.080768E-4	12.669709	19
ATAACGA	60	4.0826842E-4	12.6690035	12
GTCGGCG	90	5.384409E-7	12.6690035	12
ACCGTCG	135	2.7284841E-11	12.6683	8
TTGCGCG	100	1.4352918E-7	12.352965	18
GCGGTCG	100	1.4384023E-7	12.350904	9
TCGCCAG	185	0.0	12.327284	17
CGCATCG	185	0.0	12.323855	13
ATTTGCG	85	3.927533E-6	12.29707	16
CGAACGA	70	1.0874731E-4	12.217219	16
>>END_MODULE