FastQCFastQC Report
Thu 2 Feb 2017
SRR4062576_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062576_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences899763
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA32590.36220649215404505No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC31100.3456465758205216No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA14550.16170925010252699No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC13280.14759442208670506No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC9930.11036239543079678No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9500.10558335917347124No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACTA250.002352532535.1998255
TTAACGG1001.7444108E-924.19987935
GATATAC4100.020.3935411
TGGTATA2600.020.30758944
CGCAATA2400.019.24990336
TGTAGGA5250.018.8580952
ATACACT4450.018.7864234
GTCCTAA2250.018.5807821
CTAGATT600.00741088218.333244
CTAGTAT600.00741088218.333243
GGACCGT600.00741088218.333246
TAACGGC1456.3009793E-918.20680636
GTCCTAC4600.018.1768511
TAAGACA1601.1004886E-917.874914
GTAGGAC5800.017.8274973
TCTAGCG2850.017.75429728
TCCTATA750.001290085817.600892
CGATGAT1002.4819772E-517.59991343
CTCGAGC750.001290566617.5999112
CTATTCC2400.017.4165784
Produced by FastQC (version 0.11.5)