##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062576_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	899763
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.229058096409833	32.0	32.0	32.0	32.0	32.0
2	31.40980791608457	32.0	32.0	32.0	32.0	32.0
3	31.504925185854496	32.0	32.0	32.0	32.0	32.0
4	31.609623867618474	32.0	32.0	32.0	32.0	32.0
5	31.54144480268693	32.0	32.0	32.0	32.0	32.0
6	35.15046073243732	36.0	36.0	36.0	36.0	36.0
7	35.16318630572717	36.0	36.0	36.0	36.0	36.0
8	35.10362617711553	36.0	36.0	36.0	36.0	36.0
9	35.234949647851714	36.0	36.0	36.0	36.0	36.0
10	35.11202060987171	36.0	36.0	36.0	36.0	36.0
11	35.23799155999969	36.0	36.0	36.0	36.0	36.0
12	35.14806676869353	36.0	36.0	36.0	36.0	36.0
13	35.183104884286195	36.0	36.0	36.0	36.0	36.0
14	35.145829512882834	36.0	36.0	36.0	36.0	36.0
15	35.1119461458184	36.0	36.0	36.0	36.0	36.0
16	35.104913182693664	36.0	36.0	36.0	36.0	36.0
17	35.081114693535966	36.0	36.0	36.0	36.0	36.0
18	35.08862333747887	36.0	36.0	36.0	36.0	36.0
19	35.07590776682304	36.0	36.0	36.0	36.0	36.0
20	35.06819240177691	36.0	36.0	36.0	36.0	36.0
21	35.03137492873123	36.0	36.0	36.0	36.0	36.0
22	35.00606270762412	36.0	36.0	36.0	36.0	36.0
23	34.9407555100621	36.0	36.0	36.0	32.0	36.0
24	34.91396734473411	36.0	36.0	36.0	32.0	36.0
25	34.88477410162454	36.0	36.0	36.0	32.0	36.0
26	34.81811432566131	36.0	36.0	36.0	32.0	36.0
27	34.79091160672311	36.0	36.0	36.0	32.0	36.0
28	34.75498325670204	36.0	36.0	36.0	32.0	36.0
29	34.71884929698154	36.0	36.0	36.0	32.0	36.0
30	34.69274909059386	36.0	36.0	36.0	32.0	36.0
31	34.69152432362744	36.0	36.0	36.0	32.0	36.0
32	34.64003743207934	36.0	36.0	36.0	32.0	36.0
33	34.60233861583551	36.0	36.0	36.0	32.0	36.0
34	34.57393780362162	36.0	36.0	36.0	32.0	36.0
35	34.51439212325913	36.0	36.0	36.0	32.0	36.0
36	34.47157084698971	36.0	36.0	36.0	32.0	36.0
37	34.41623627555256	36.0	36.0	36.0	32.0	36.0
38	34.340774181645614	36.0	36.0	36.0	32.0	36.0
39	34.23303358773366	36.0	36.0	36.0	32.0	36.0
40	34.16951130464356	36.0	36.0	36.0	32.0	36.0
41	34.16751077783817	36.0	36.0	36.0	32.0	36.0
42	34.034292363655766	36.0	36.0	36.0	32.0	36.0
43	34.07270025551173	36.0	36.0	36.0	32.0	36.0
44	34.04574982523175	36.0	36.0	36.0	32.0	36.0
45	33.88771821023981	36.0	36.0	36.0	32.0	36.0
46	33.980992772541214	36.0	36.0	36.0	32.0	36.0
47	33.89749967491439	36.0	36.0	36.0	32.0	36.0
48	33.88802606908708	36.0	36.0	36.0	32.0	36.0
49	33.92505915446623	36.0	36.0	36.0	32.0	36.0
50	33.35097575694933	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	1.0
21	23.0
22	78.0
23	252.0
24	749.0
25	1785.0
26	4082.0
27	7466.0
28	12429.0
29	17928.0
30	24897.0
31	34282.0
32	50137.0
33	72151.0
34	152958.0
35	520542.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.97615101504081	17.35123424471915	11.947057587712132	25.725557152527905
2	15.247625112950928	20.543231446872216	37.18928074849371	27.019862691683144
3	19.500465680771942	24.029127832150422	28.3377308120628	28.13267567501484
4	12.34429181114964	15.81366142082333	35.410665066239886	36.43138170178714
5	13.842311808776312	36.94917439370145	33.90270549022354	15.3058083072987
6	34.82299645681718	34.492684683403056	16.807223291647958	13.877095568131805
7	29.646061958606833	30.467723686930544	20.75893402922106	19.127280325241564
8	28.38414115717139	33.38957036464046	19.777763699996555	18.44852477819159
9	26.962544581184154	14.640966565640062	18.184455239879835	40.212033613295944
10	15.58754916572475	27.350091079539833	32.07222346328978	24.990136291445637
11	37.72704589986474	20.930845122548938	21.35006662865666	19.99204234892966
12	23.71657869905742	23.744141512820597	29.324611036461825	23.21466875166016
13	30.846789654609047	19.269741031805044	25.05515341262088	24.82831590096503
14	23.630444906047483	19.448343619375326	24.778413871208308	32.14279760336889
15	25.611077583763723	27.066683115442626	23.37871194970231	23.943527351091344
16	25.91760274650102	25.519942473740308	24.894888987433358	23.667565792325313
17	23.727137034974767	25.891262476896692	26.447408928795696	23.934191559332845
18	25.268209517395135	24.023548423307027	27.94658148868091	22.76166057061693
19	25.122837902869975	26.359496889736523	25.005029102108	23.5126361052855
20	24.669940862204825	24.173810214467586	27.304190103393893	23.852058819933696
21	25.701768132274832	25.211416784197617	23.911074360692762	25.17574072283479
22	24.416540800188493	25.66386926335046	25.2497602146343	24.669829721826748
23	23.53908751526791	24.804420719678404	25.248982231987753	26.40750953306593
24	23.7598123061295	26.380946982705446	25.326335935129585	24.532904776035465
25	25.292438119815998	24.324183146006227	24.93523294467543	25.448145789502348
26	23.166322687196516	25.379572176228628	26.182450267459316	25.271654869115533
27	25.18018633795788	25.49015685241558	25.789791311712086	23.53986549791445
28	24.159806526829843	24.383087546387216	25.835247726345717	25.621858200437224
29	23.447730124488338	25.451591141222746	26.454521912992647	24.646156821296277
30	24.850655116958578	24.900001444824916	26.296591435744748	23.95275200247176
31	23.92618945211128	25.055486833755108	25.409024376419126	25.609299337714486
32	23.837388290027487	25.58240336621977	25.629415746146485	24.95079259760626
33	23.578986910997674	24.990914274092177	26.160889034112316	25.26920978079783
34	23.72313598136398	25.869367822415455	26.889747633543497	23.51774856267706
35	25.308886895771444	25.05093007825394	26.16577921074772	23.4744038152269
36	23.804824159250828	25.963170301512733	25.806017806911374	24.42598773232507
37	25.05170806090048	25.33578286726616	25.882593527406662	23.7299155444267
38	24.335519464570115	25.335338305753847	25.119614831905736	25.209527397770305
39	24.457218178564798	24.81875782845038	25.99284478245938	24.73117921052544
40	25.54861669128426	25.139175538447343	25.219307751041107	24.092900019227283
41	23.31491737268592	25.11350211111148	27.018114770222823	24.553465745979775
42	25.865366768804677	25.75189244278771	25.607298810909096	22.77544197749852
43	24.391534215121094	24.13413309949398	25.853141327216168	25.62119135816876
44	23.87550943970801	25.090051491337164	26.722036803024796	24.31240226593003
45	24.410205798638085	25.28299118767942	25.78401201205206	24.522791001630427
46	23.66695155713573	24.657659089469313	26.794448748056425	24.880940605338527
47	24.848571066570937	24.315652361402236	26.881997683831948	23.95377888819488
48	24.287062259728394	26.683359951453884	24.643711732978574	24.38586605583915
49	23.5043005769297	25.06371122173283	27.15281690845256	24.279171292884904
50	23.268153133828722	27.0510423867645	24.490476370417955	25.190328108988822
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	21.0
2	24.0
3	91.0
4	158.0
5	178.0
6	198.0
7	191.0
8	184.0
9	231.5
10	279.0
11	420.5
12	562.0
13	993.0
14	1424.0
15	1906.0
16	2388.0
17	2756.0
18	3124.0
19	3328.0
20	3532.0
21	4011.0
22	4490.0
23	4821.0
24	5152.0
25	5789.0
26	6426.0
27	8431.5
28	10437.0
29	12147.0
30	13857.0
31	15164.5
32	16472.0
33	18942.5
34	21413.0
35	24683.5
36	27954.0
37	31508.0
38	35062.0
39	36936.0
40	38810.0
41	41701.0
42	44592.0
43	46735.5
44	48879.0
45	56989.5
46	65100.0
47	71142.5
48	77185.0
49	75182.0
50	73179.0
51	67311.0
52	61443.0
53	59108.0
54	56773.0
55	57254.5
56	57736.0
57	55749.0
58	53762.0
59	49808.5
60	45855.0
61	40627.0
62	35399.0
63	30975.0
64	26551.0
65	22562.0
66	18573.0
67	15942.5
68	13312.0
69	12068.5
70	10825.0
71	8571.0
72	6317.0
73	5650.0
74	4983.0
75	3599.5
76	2216.0
77	2072.0
78	1928.0
79	1628.0
80	1328.0
81	1062.5
82	797.0
83	664.5
84	532.0
85	426.5
86	321.0
87	237.0
88	153.0
89	94.5
90	36.0
91	23.0
92	10.0
93	8.5
94	7.0
95	5.5
96	4.0
97	3.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016004214443136693
2	0.004001053610784173
3	5.557018903866907E-4
4	3.334211342320144E-4
5	0.0
6	7.77982646541367E-4
7	1.1114037807733815E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.222807561546763E-4
47	1.1114037807733815E-4
48	0.0
49	0.0
50	1.1114037807733815E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	899763.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.83430061785047
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.09389075206047	46.90008456025789
2	11.611955205530911	13.431386162353768
3	3.0701437653980284	5.3267885240414685
4	1.3327299923298646	3.0831002807532366
5	0.7144294786897964	2.065926462039994
6	0.446206932197972	1.5483639512703797
7	0.3122769654675255	1.2642223927815286
8	0.19912515060854724	0.9213011056695577
9	0.16739233630233943	0.8712916828950441
>10	0.8848945420856056	9.414670442478162
>50	0.08338295905678149	3.372506390296733
>100	0.07699450065905199	8.553975739261595
>500	0.005803605540987327	2.22243376497143
>1k	7.738140721316435E-4	1.0239485409292453
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3259	0.36220649215404505	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	3110	0.3456465758205216	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1455	0.16170925010252699	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1328	0.14759442208670506	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	993	0.11036239543079678	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	950	0.10558335917347124	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.1114037807733814E-4	0.0
11	0.0	0.0	0.0	1.1114037807733814E-4	0.0
12	0.0	0.0	0.0	1.1114037807733814E-4	3.334211342320144E-4
13	0.0	0.0	0.0	2.2228075615467627E-4	3.334211342320144E-4
14	0.0	0.0	0.0	2.2228075615467627E-4	3.334211342320144E-4
15	0.0	0.0	0.0	3.334211342320144E-4	4.4456151230935255E-4
16	0.0	0.0	0.0	3.334211342320144E-4	4.4456151230935255E-4
17	0.0	0.0	0.0	4.4456151230935255E-4	4.4456151230935255E-4
18	0.0	0.0	0.0	4.4456151230935255E-4	4.4456151230935255E-4
19	0.0	0.0	0.0	4.4456151230935255E-4	4.4456151230935255E-4
20	0.0	0.0	0.0	7.77982646541367E-4	6.668422684640288E-4
21	0.0	0.0	0.0	0.0014448249150053958	6.668422684640288E-4
22	0.0	0.0	0.0	0.003223070964242806	6.668422684640288E-4
23	0.0	0.0	0.0	0.0051124573915575545	6.668422684640288E-4
24	0.0	0.0	0.0	0.008002107221568347	6.668422684640288E-4
25	0.0	0.0	0.0	0.00955807251465108	6.668422684640288E-4
26	0.0	0.0	0.0	0.012114301210429858	6.668422684640288E-4
27	0.0	0.0	0.0	0.015226231796595326	6.668422684640288E-4
28	0.0	0.0	0.0	0.022561496749699643	6.668422684640288E-4
29	0.0	0.0	0.0	0.03545378060667087	6.668422684640288E-4
30	0.0	0.0	0.0	0.058237558112525185	6.668422684640288E-4
31	0.0	0.0	0.0	0.10224914783115109	6.668422684640288E-4
32	0.0	0.0	0.0	0.15804161762597485	6.668422684640288E-4
33	0.0	0.0	0.0	0.2176128602754281	6.668422684640288E-4
34	0.0	0.0	0.0	0.2851862101464497	6.668422684640288E-4
35	0.0	0.0	0.0	0.36120622875134895	6.668422684640288E-4
36	0.0	0.0	0.0	0.4790150295133274	6.668422684640288E-4
37	0.0	0.0	0.0	0.6592847227547699	6.668422684640288E-4
38	0.0	0.0	0.0	0.8566700342201224	7.77982646541367E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGACTA	25	0.0023525325	35.199825	5
TTAACGG	100	1.7444108E-9	24.199879	35
GATATAC	410	0.0	20.393541	1
TGGTATA	260	0.0	20.307589	44
CGCAATA	240	0.0	19.249903	36
TGTAGGA	525	0.0	18.858095	2
ATACACT	445	0.0	18.786423	4
GTCCTAA	225	0.0	18.580782	1
CTAGATT	60	0.007410882	18.33324	4
CTAGTAT	60	0.007410882	18.33324	3
GGACCGT	60	0.007410882	18.33324	6
TAACGGC	145	6.3009793E-9	18.206806	36
GTCCTAC	460	0.0	18.176851	1
TAAGACA	160	1.1004886E-9	17.87491	4
GTAGGAC	580	0.0	17.827497	3
TCTAGCG	285	0.0	17.754297	28
TCCTATA	75	0.0012900858	17.60089	2
CGATGAT	100	2.4819772E-5	17.599913	43
CTCGAGC	75	0.0012905666	17.59991	12
CTATTCC	240	0.0	17.416578	4
>>END_MODULE