FastQCFastQC Report
Thu 2 Feb 2017
SRR4062574_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062574_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191104
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36030.16443765334735366No Hit
TATCAACGCAGAGTACTTTTTTTTT23760.1084384858044164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCAGT4300.013.25721618
CGCATCG4750.012.60104613
GCGTTAT1405.2750693E-1112.2080471
CGGTCCA4450.011.95632210
AACGCCG5550.011.8107115
CCGCATC5200.011.69301112
TAGATCG658.0262823E-411.6922095
TCGCCAG5050.011.664617
TAACGCC5550.011.6392764
GTACCGT907.4536583E-611.6126066
GCATCGC4950.011.51610714
ATAACGC5650.011.4296153
CGAACGA1251.8295395E-811.40146716
GTATTAA1604.5474735E-1111.2754881
CCGACCA3050.011.2143019
AGAATTT4900.011.0521917
ATCGCCA5250.011.039515516
CGACCAT3100.011.03342510
CAAGACG4050.011.0243464
ACGCTTA951.3591916E-511.00116417
Produced by FastQC (version 0.11.5)