##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062574_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2191104
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17566487031195	32.0	32.0	32.0	32.0	32.0
2	30.753836878578106	32.0	32.0	32.0	32.0	32.0
3	30.74562184177474	32.0	32.0	32.0	32.0	32.0
4	30.83683841570277	32.0	32.0	32.0	32.0	32.0
5	30.58249129206099	32.0	32.0	32.0	32.0	32.0
6	34.262055110118006	36.0	36.0	36.0	32.0	36.0
7	34.14294894263349	36.0	36.0	36.0	32.0	36.0
8	34.1410252548487	36.0	36.0	36.0	32.0	36.0
9	34.331690782363594	36.0	36.0	36.0	32.0	36.0
10	33.928468479816566	36.0	36.0	36.0	32.0	36.0
11	34.30488192253768	36.0	36.0	36.0	32.0	36.0
12	34.04760020519336	36.0	36.0	36.0	32.0	36.0
13	34.182124627585	36.0	36.0	36.0	32.0	36.0
14	34.08326441830237	36.0	36.0	36.0	32.0	36.0
15	34.007436890261715	36.0	36.0	36.0	32.0	36.0
16	34.01151611242552	36.0	36.0	36.0	32.0	36.0
17	33.93195758850333	36.0	36.0	36.0	32.0	36.0
18	33.94185351311485	36.0	36.0	36.0	32.0	36.0
19	33.943779026463375	36.0	36.0	36.0	32.0	36.0
20	33.93408026273513	36.0	36.0	36.0	32.0	36.0
21	33.91773142671457	36.0	36.0	36.0	32.0	36.0
22	33.86742619245823	36.0	36.0	36.0	32.0	36.0
23	33.81605848010866	36.0	36.0	36.0	32.0	36.0
24	33.79276885077112	36.0	36.0	36.0	32.0	36.0
25	33.33950510792733	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	15.0
4	94.0
5	229.0
6	383.0
7	110.0
8	325.0
9	437.0
10	250.0
11	76.0
12	162.0
13	131.0
14	398.0
15	479.0
16	701.0
17	1005.0
18	1339.0
19	1941.0
20	2864.0
21	4233.0
22	6637.0
23	9942.0
24	13996.0
25	19915.0
26	28035.0
27	36210.0
28	48413.0
29	64473.0
30	81901.0
31	111816.0
32	159710.0
33	228433.0
34	498533.0
35	867917.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.42264498004249	17.35524817369991	10.891161415031688	26.33094543122591
2	17.266305585589862	19.848490173266885	36.29608770007298	26.589116541070272
3	19.229442113680427	23.265391257991844	28.283273634129685	29.22189299419804
4	13.03630581476398	14.966237605277824	35.40927289317681	36.588183686781385
5	14.97070891680748	36.0623890255801	33.54451098661025	15.42239107100217
6	34.680725350652814	34.85387144997986	16.61357827052982	13.8518249288375
7	30.594054431773028	30.145510680374326	20.714308550236808	18.546126337615835
8	28.304209612221225	32.567725042471615	19.536076093058867	19.59198925224829
9	27.38875414418024	14.25036079551814	18.541486116625013	39.81939894367661
10	16.056203131118856	26.454486092471324	31.5387501205208	25.95056065588902
11	37.551259707014275	21.047484744325217	22.097811791994577	19.303443756665935
12	24.55501327864964	23.65254040150834	28.57567230733162	23.216774012510403
13	29.48718768906818	19.314565584153275	25.234467392079196	25.963779334699343
14	23.428038464543953	19.496403831100018	24.800244573301388	32.27531313105464
15	25.206462408169124	27.04253166731703	22.13148162194419	25.619524302569662
16	25.904158078537577	25.649746891024922	23.60371984978408	24.842375180653416
17	24.25977665119365	25.6640463989369	25.109330145508483	24.96684680436096
18	24.955076821833668	24.816102880019013	26.076145472493124	24.152674825654195
19	25.66923736075407	24.990939731505286	25.2278548986004	24.111968009140245
20	25.557667236195925	24.48343666047176	24.9771860806989	24.98171002263342
21	26.4350843903839	24.25526605866836	24.41864503757488	24.891004513372856
22	25.743920215788172	24.516101606206696	24.678829510979792	25.06114866702534
23	24.506937068204294	24.369045812621458	25.352840733543086	25.771176385631158
24	24.80899860288025	25.02343386166732	25.048021706771213	25.11954582868122
25	24.918150559098862	24.645136287773678	25.1714679521279	25.26524520099956
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	326.0
1	326.0
2	752.0
3	1178.0
4	1178.0
5	1178.0
6	2719.5
7	4261.0
8	4261.0
9	4261.0
10	4753.0
11	5245.0
12	5245.0
13	5245.0
14	6146.0
15	7047.0
16	7047.0
17	7047.0
18	11034.5
19	15022.0
20	15022.0
21	15022.0
22	23809.0
23	32596.0
24	32596.0
25	32596.0
26	47539.0
27	62482.0
28	62482.0
29	62482.0
30	77131.0
31	91780.0
32	91780.0
33	91780.0
34	113121.0
35	134462.0
36	134462.0
37	134462.0
38	153540.5
39	172619.0
40	172619.0
41	172619.0
42	196657.0
43	220695.0
44	220695.0
45	220695.0
46	250836.0
47	280977.0
48	280977.0
49	280977.0
50	284632.0
51	288287.0
52	288287.0
53	288287.0
54	271329.0
55	254371.0
56	254371.0
57	254371.0
58	234330.5
59	214290.0
60	214290.0
61	214290.0
62	189990.0
63	165690.0
64	165690.0
65	165690.0
66	138392.0
67	111094.0
68	111094.0
69	111094.0
70	85341.0
71	59588.0
72	59588.0
73	59588.0
74	46637.0
75	33686.0
76	33686.0
77	33686.0
78	26876.5
79	20067.0
80	20067.0
81	20067.0
82	14332.5
83	8598.0
84	8598.0
85	8598.0
86	6349.5
87	4101.0
88	4101.0
89	4101.0
90	2756.5
91	1412.0
92	1412.0
93	1412.0
94	860.5
95	309.0
96	309.0
97	309.0
98	615.0
99	921.0
100	921.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009082179577053394
2	0.004153157495034467
3	0.005476691202243253
4	0.016110599953265567
5	0.038656312069166955
6	0.04773849164622035
7	0.07183593293609067
8	0.09104999123729408
9	0.09999525353429138
10	0.12254096565019276
11	0.12495983759785022
12	0.13714547552284145
13	0.12505111578455427
14	0.12792637866573198
15	0.11729246991470967
16	0.11505615434046032
17	0.1267854013319313
18	0.13344870896132727
19	0.13595885909568875
20	0.12537058943801846
21	0.13417893445495968
22	0.15530983467694826
23	0.14367186587218134
24	0.13833209194999416
25	0.13417893445495968
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2191104.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.80731545830167
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.18727068710145	41.816671834556914
2	12.307643074134239	12.998671807279772
3	3.5928561914169745	5.691872708894054
4	1.5034131584534882	3.1756485169046016
5	0.8156036931939815	2.1534920757725238
6	0.5202270923704228	1.6483077706055973
7	0.3439758331060834	1.271510823020555
8	0.27125869996525415	1.1459554991899186
9	0.19662960855888478	0.9345133590850265
>10	1.0713208869553317	10.178755771460848
>50	0.09405677514817269	3.4662142591030642
>100	0.08178480920504648	9.137528276987748
>500	0.01066470383875641	3.7292674851264604
>1k	0.0032947865518109156	2.651589812012955
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3603	0.16443765334735366	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2376	0.1084384858044164	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8255637340810842E-4
2	0.0	0.0	0.0	0.0	1.8255637340810842E-4
3	0.0	0.0	0.0	0.0	1.8255637340810842E-4
4	0.0	0.0	0.0	0.0	1.8255637340810842E-4
5	0.0	4.5639093352027105E-5	0.0	0.0	1.8255637340810842E-4
6	0.0	4.5639093352027105E-5	0.0	0.0	1.8255637340810842E-4
7	0.0	4.5639093352027105E-5	0.0	4.5639093352027105E-5	1.8255637340810842E-4
8	0.0	4.5639093352027105E-5	0.0	9.127818670405421E-5	1.8255637340810842E-4
9	0.0	4.5639093352027105E-5	0.0	1.8255637340810842E-4	2.2819546676013553E-4
10	0.0	4.5639093352027105E-5	0.0	1.8255637340810842E-4	2.2819546676013553E-4
11	0.0	4.5639093352027105E-5	0.0	1.8255637340810842E-4	2.2819546676013553E-4
12	0.0	4.5639093352027105E-5	0.0	1.8255637340810842E-4	3.1947365346418974E-4
13	0.0	4.5639093352027105E-5	0.0	1.8255637340810842E-4	3.6511274681621684E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCAGT	430	0.0	13.257216	18
CGCATCG	475	0.0	12.601046	13
GCGTTAT	140	5.2750693E-11	12.208047	1
CGGTCCA	445	0.0	11.956322	10
AACGCCG	555	0.0	11.810711	5
CCGCATC	520	0.0	11.693011	12
TAGATCG	65	8.0262823E-4	11.692209	5
TCGCCAG	505	0.0	11.6646	17
TAACGCC	555	0.0	11.639276	4
GTACCGT	90	7.4536583E-6	11.612606	6
GCATCGC	495	0.0	11.516107	14
ATAACGC	565	0.0	11.429615	3
CGAACGA	125	1.8295395E-8	11.401467	16
GTATTAA	160	4.5474735E-11	11.275488	1
CCGACCA	305	0.0	11.214301	9
AGAATTT	490	0.0	11.05219	17
ATCGCCA	525	0.0	11.0395155	16
CGACCAT	310	0.0	11.033425	10
CAAGACG	405	0.0	11.024346	4
ACGCTTA	95	1.3591916E-5	11.001164	17
>>END_MODULE