FastQCFastQC Report
Thu 2 Feb 2017
SRR4062574_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062574_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191104
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG2000.019.8007812
TAGATCG1900.019.6840865
TAGGACG4700.018.7237154
GTATCAA37850.018.4867481
TACCGTC5050.016.9899947
TTAACGG3550.016.73228835
ATACCGT5150.016.6600916
TATAACG800.001988521816.500652
TAACGGC3600.016.49989736
ATACGAA6300.016.41259640
GTTATAG5800.016.3132211
CTATACA2850.016.2107944
CGCAATA6150.016.0974636
TGTAGGA7750.015.8974012
GTCCTAA3750.015.84279251
ATCGATT1259.447747E-615.83990229
AACGCAG43500.015.8297866
AGGACGT5500.015.5999035
CTAGCGG6800.015.52931429
TGCGTAG2700.015.48138513
Produced by FastQC (version 0.11.5)