##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062574_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2191104
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31491522082019	32.0	32.0	32.0	32.0	32.0
2	31.446861034437433	32.0	32.0	32.0	32.0	32.0
3	31.51795213737002	32.0	32.0	32.0	32.0	32.0
4	31.611651934367334	32.0	32.0	32.0	32.0	32.0
5	31.553857324891926	32.0	32.0	32.0	32.0	32.0
6	35.151385329039606	36.0	36.0	36.0	36.0	36.0
7	35.17354310886201	36.0	36.0	36.0	36.0	36.0
8	35.109709990945206	36.0	36.0	36.0	36.0	36.0
9	35.232960188106084	36.0	36.0	36.0	36.0	36.0
10	35.11016181796939	36.0	36.0	36.0	36.0	36.0
11	35.245642835757685	36.0	36.0	36.0	36.0	36.0
12	35.15138441625774	36.0	36.0	36.0	36.0	36.0
13	35.19425960611637	36.0	36.0	36.0	36.0	36.0
14	35.16108865667718	36.0	36.0	36.0	36.0	36.0
15	35.119393693772636	36.0	36.0	36.0	36.0	36.0
16	35.1367105349632	36.0	36.0	36.0	36.0	36.0
17	35.11169483511508	36.0	36.0	36.0	36.0	36.0
18	35.119756524564785	36.0	36.0	36.0	36.0	36.0
19	35.11242414782685	36.0	36.0	36.0	36.0	36.0
20	35.09498590664797	36.0	36.0	36.0	36.0	36.0
21	35.10198420522257	36.0	36.0	36.0	36.0	36.0
22	35.08248398980605	36.0	36.0	36.0	36.0	36.0
23	35.04307326352377	36.0	36.0	36.0	36.0	36.0
24	35.022201136961094	36.0	36.0	36.0	36.0	36.0
25	34.99182740755345	36.0	36.0	36.0	36.0	36.0
26	34.9571918996086	36.0	36.0	36.0	32.0	36.0
27	34.93819827812828	36.0	36.0	36.0	32.0	36.0
28	34.92722025061339	36.0	36.0	36.0	32.0	36.0
29	34.90672327739806	36.0	36.0	36.0	32.0	36.0
30	34.88919056329595	36.0	36.0	36.0	32.0	36.0
31	34.891029818758035	36.0	36.0	36.0	32.0	36.0
32	34.863812489046616	36.0	36.0	36.0	32.0	36.0
33	34.839197044047204	36.0	36.0	36.0	32.0	36.0
34	34.83374362878257	36.0	36.0	36.0	32.0	36.0
35	34.80474363608482	36.0	36.0	36.0	32.0	36.0
36	34.77780196649726	36.0	36.0	36.0	32.0	36.0
37	34.77178217008412	36.0	36.0	36.0	32.0	36.0
38	34.74042172347821	36.0	36.0	36.0	32.0	36.0
39	34.73280592797056	36.0	36.0	36.0	32.0	36.0
40	34.72215285080033	36.0	36.0	36.0	32.0	36.0
41	34.693553569342214	36.0	36.0	36.0	32.0	36.0
42	34.67773825432293	36.0	36.0	36.0	32.0	36.0
43	34.6659318772637	36.0	36.0	36.0	32.0	36.0
44	34.64217901112864	36.0	36.0	36.0	32.0	36.0
45	34.603254341190556	36.0	36.0	36.0	32.0	36.0
46	34.60175692253768	36.0	36.0	36.0	32.0	36.0
47	34.57476368077462	36.0	36.0	36.0	32.0	36.0
48	34.53829028654048	36.0	36.0	36.0	32.0	36.0
49	34.53450224179227	36.0	36.0	36.0	32.0	36.0
50	34.02784943115434	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	9.0
21	51.0
22	185.0
23	509.0
24	1251.0
25	3110.0
26	6397.0
27	12042.0
28	20398.0
29	31599.0
30	46161.0
31	66392.0
32	97960.0
33	162471.0
34	364109.0
35	1378453.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.90279973854013	17.91068552878067	11.388612984622801	26.797901748056397
2	16.701954854548617	20.28291100849974	36.066977421797375	26.948156715154266
3	18.66421796264966	23.6188729724142	28.55490712161202	29.16200194332412
4	12.782124404237324	15.43385305730138	35.59172816928856	36.19229436917273
5	14.616786788760368	36.34195364528566	33.4664625686412	15.57479699731277
6	34.874300120670426	35.06201473968231	16.352896181447424	13.710788958199839
7	30.432786394438605	30.299520241850686	20.690893722981656	18.576799640729057
8	28.365015991938307	32.77822504089263	19.342669266269425	19.514089700899635
9	27.55149915293843	14.033884288468279	18.451794164037853	39.96282239455544
10	15.949767788293027	26.572266765977332	31.59854575592943	25.87941968980021
11	37.78241470966234	20.942730240098143	22.032956902091367	19.24189814814815
12	24.601844549596915	23.582997429606262	28.54131980955719	23.27383821123963
13	29.619817224558943	19.1811525148966	25.203230882696577	25.995799377847877
14	23.511389692136934	19.353440092300502	24.86066384799626	32.2745063675663
15	25.281593205982006	27.06393671865872	22.087860731393853	25.566609343965418
16	26.020900879191494	25.59180212349574	23.569533897067416	24.817763100245354
17	24.342888333917514	25.648120764692138	25.0606999941582	24.948290907232153
18	25.121536905596447	24.674273790746582	26.03833501285197	24.165854290805
19	25.773719549596912	24.897631513611405	25.226187346652644	24.102461590139036
20	25.67769489718425	24.35521088912256	25.025284057717023	24.941810155976167
21	26.526536394438605	24.13185316625774	24.44347689566538	24.898133543638277
22	25.770479173968923	24.4652467431943	24.744421077228647	25.019853005608134
23	24.582265378548897	24.22687375861666	25.43585334151186	25.755007521322586
24	24.94600895256455	24.880060462670873	25.042444356817384	25.131486227947192
25	25.01889458464774	24.4337557687814	25.250010953382407	25.297338693188458
26	24.65953236359388	25.010725186937727	25.60951009171632	24.720232357752074
27	25.464925443977098	24.766601676597734	24.75076491120458	25.017707968220588
28	24.659349807220472	24.5508656823227	25.522750175254117	25.26703433520271
29	24.716900703937377	24.816850318378314	25.339098463605563	25.12715051407875
30	24.765734533824045	24.90790030961561	25.5065939362075	24.819771220352845
31	25.14093352027106	24.617635675896718	24.851262194765745	25.39016860906648
32	24.864999561864703	24.771667615959807	24.875313996962262	25.488018825213228
33	24.62648966000701	24.41390276317327	25.352333800677652	25.60727377614207
34	25.061065106905012	24.551687186003036	25.587740244187408	24.799507462904543
35	25.63465723215329	24.454795390816685	25.242206668419207	24.66834070861082
36	24.650267627643416	24.8198168594462	25.221167046383925	25.308748466526467
37	25.57048866690034	24.551093877789462	24.814066333683844	25.06435112162636
38	24.752088444911788	24.47227516357051	25.45095988141138	25.32467651010632
39	25.174843366631617	24.391311411964015	24.925745195116253	25.508100026288115
40	25.45524995618647	24.406919981890407	25.463464992989838	24.674365068933287
41	24.52339094812478	24.683584165790396	25.75564646862951	25.037378417455308
42	25.436720484285548	24.882844447365347	25.292409671106437	24.388025397242668
43	24.729040702769016	24.05937828601472	25.60951009171632	25.60207091949994
44	24.483639297815166	24.49974989776843	25.426177853721228	25.590432950695174
45	24.448907947774273	24.8614853516766	25.45319619698563	25.2364105035635
46	24.386803152208092	24.354171125996587	25.651191479709496	25.60783424208582
47	24.63894913249211	24.289992624722515	25.881153975347587	25.189904267437786
48	25.02560353137049	25.069462700081786	25.109168711298047	24.795765057249678
49	24.241341351209254	24.875861666082486	25.446167776609418	25.436629206098843
50	23.96474468098701	25.229815864345884	25.360343790476207	25.445095664190898
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38.0
1	51.0
2	64.0
3	181.0
4	298.0
5	365.5
6	433.0
7	390.5
8	348.0
9	474.5
10	601.0
11	858.0
12	1115.0
13	1911.0
14	2707.0
15	3590.5
16	4474.0
17	5060.0
18	5646.0
19	6022.5
20	6399.0
21	7577.0
22	8755.0
23	9570.5
24	10386.0
25	12920.0
26	15454.0
27	19050.5
28	22647.0
29	26026.0
30	29405.0
31	31768.5
32	34132.0
33	39661.5
34	45191.0
35	52508.5
36	59826.0
37	70802.0
38	81778.0
39	88454.5
40	95131.0
41	103384.5
42	111638.0
43	115015.5
44	118393.0
45	129197.5
46	140002.0
47	147999.0
48	155996.0
49	160068.0
50	164140.0
51	159975.0
52	155810.0
53	153823.0
54	151836.0
55	152603.5
56	153371.0
57	150132.5
58	146894.0
59	135035.0
60	123176.0
61	108363.0
62	93550.0
63	82479.0
64	71408.0
65	60619.0
66	49830.0
67	44759.0
68	39688.0
69	36022.0
70	32356.0
71	25869.0
72	19382.0
73	17023.0
74	14664.0
75	11232.5
76	7801.0
77	6891.0
78	5981.0
79	5090.5
80	4200.0
81	3317.5
82	2435.0
83	2096.0
84	1757.0
85	1458.5
86	1160.0
87	849.0
88	538.0
89	361.5
90	185.0
91	113.0
92	41.0
93	29.5
94	18.0
95	14.5
96	11.0
97	9.0
98	7.0
99	7.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014969622619464893
2	0.004426992055146629
3	5.933082135763524E-4
4	2.2819546676013553E-4
5	0.0
6	5.476691202243253E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.2819546676013553E-4
47	0.0
48	0.0
49	0.0
50	9.127818670405421E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2191104.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.778777638030284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.85719335715952	44.543366310661895
2	12.23985166034416	13.654479281696252
3	3.3557657657515874	5.615415373595166
4	1.4144259812329796	3.1557980917058868
5	0.7760576767659291	2.164377429330657
6	0.4692867626955175	1.5705745190918614
7	0.32463166462607346	1.2675290204808958
8	0.24709750608325343	1.1026237477383702
9	0.1833146990560365	0.920256285278619
>10	0.9726296397387358	9.790806633708167
>50	0.07879057400206088	3.060917070806916
>100	0.07016959745507649	8.22346747981543
>500	0.008562228772960806	3.1730847572700607
>1k	0.002222886316057104	1.7573039988198071
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.5639093352027105E-5
2	0.0	0.0	0.0	0.0	4.5639093352027105E-5
3	0.0	0.0	0.0	0.0	4.5639093352027105E-5
4	0.0	0.0	0.0	0.0	9.127818670405421E-5
5	0.0	4.5639093352027105E-5	0.0	0.0	9.127818670405421E-5
6	0.0	4.5639093352027105E-5	0.0	0.0	9.127818670405421E-5
7	0.0	4.5639093352027105E-5	0.0	0.0	9.127818670405421E-5
8	0.0	4.5639093352027105E-5	0.0	0.0	1.3691728005608132E-4
9	0.0	4.5639093352027105E-5	0.0	0.0	1.8255637340810842E-4
10	0.0	4.5639093352027105E-5	0.0	0.0	1.8255637340810842E-4
11	0.0	4.5639093352027105E-5	0.0	0.0	1.8255637340810842E-4
12	0.0	4.5639093352027105E-5	0.0	0.0	3.6511274681621684E-4
13	0.0	4.5639093352027105E-5	0.0	0.0	4.1075184016824395E-4
14	0.0	4.5639093352027105E-5	0.0	0.0	5.020300268722982E-4
15	0.0	4.5639093352027105E-5	0.0	4.5639093352027105E-5	5.020300268722982E-4
16	0.0	4.5639093352027105E-5	0.0	4.5639093352027105E-5	5.476691202243253E-4
17	0.0	4.5639093352027105E-5	0.0	4.5639093352027105E-5	5.933082135763524E-4
18	0.0	4.5639093352027105E-5	0.0	9.127818670405421E-5	6.389473069283795E-4
19	0.0	4.5639093352027105E-5	0.0	1.3691728005608132E-4	6.389473069283795E-4
20	0.0	9.127818670405421E-5	0.0	6.389473069283795E-4	6.845864002804066E-4
21	0.0	9.127818670405421E-5	0.0	7.302254936324337E-4	6.845864002804066E-4
22	0.0	9.127818670405421E-5	0.0	0.0010040600537445964	6.845864002804066E-4
23	0.0	9.127818670405421E-5	0.0	0.0013235337072087861	7.302254936324337E-4
24	0.0	9.127818670405421E-5	0.0	0.0020993982941932467	7.302254936324337E-4
25	0.0	9.127818670405421E-5	0.0	0.003012180161233789	7.302254936324337E-4
26	0.0	9.127818670405421E-5	0.0	0.004472631148498656	7.302254936324337E-4
27	0.0	9.127818670405421E-5	0.0	0.006069999415819605	8.671427736885151E-4
28	0.0	9.127818670405421E-5	0.0	0.012642028858511508	8.671427736885151E-4
29	0.0	9.127818670405421E-5	0.0	0.027429095104568292	8.671427736885151E-4
30	0.0	9.127818670405421E-5	0.0	0.05618172391634537	8.671427736885151E-4
31	0.0	9.127818670405421E-5	0.0	0.11797705631499007	8.671427736885151E-4
32	0.0	9.127818670405421E-5	0.0	0.19232313938544224	9.584209603925693E-4
33	0.0	9.127818670405421E-5	0.0	0.26799275616310314	9.584209603925693E-4
34	0.0	9.127818670405421E-5	0.0	0.35502650718541884	0.0010040600537445964
35	0.0	9.127818670405421E-5	0.0	0.4537438661058535	0.0010040600537445964
36	0.0	9.127818670405421E-5	0.0	0.5904785897885267	0.0010040600537445964
37	0.0	9.127818670405421E-5	0.0	0.791336239630798	0.0010040600537445964
38	0.0	9.127818670405421E-5	0.0	1.0600135821941816	0.0010953382404486505
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACG	200	0.0	19.800781	2
TAGATCG	190	0.0	19.684086	5
TAGGACG	470	0.0	18.723715	4
GTATCAA	3785	0.0	18.486748	1
TACCGTC	505	0.0	16.989994	7
TTAACGG	355	0.0	16.732288	35
ATACCGT	515	0.0	16.660091	6
TATAACG	80	0.0019885218	16.50065	2
TAACGGC	360	0.0	16.499897	36
ATACGAA	630	0.0	16.412596	40
GTTATAG	580	0.0	16.313221	1
CTATACA	285	0.0	16.210794	4
CGCAATA	615	0.0	16.09746	36
TGTAGGA	775	0.0	15.897401	2
GTCCTAA	375	0.0	15.8427925	1
ATCGATT	125	9.447747E-6	15.839902	29
AACGCAG	4350	0.0	15.829786	6
AGGACGT	550	0.0	15.599903	5
CTAGCGG	680	0.0	15.529314	29
TGCGTAG	270	0.0	15.481385	13
>>END_MODULE