FastQCFastQC Report
Thu 2 Feb 2017
SRR4062573_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062573_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397025
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG96102.420502487248914No Hit
TCCATGTACTCTGCGTTGATACCAC78401.9746867325735156No Hit
GAGTACATGGAAGCAGTGGTATCAA41441.0437629872174297No Hit
CATGTACTCTGCGTTGATACCACTG34660.8729928845790568No Hit
GCTTCCATGTACTCTGCGTTGATAC26290.6621749260122158No Hit
GTATCAACGCAGAGTACTTTTTTTT23540.592909766387507No Hit
CCCATGTACTCTGCGTTGATACCAC23220.5848498205402682No Hit
CATGGAAGCAGTGGTATCAACGCAG23010.5795604810780177No Hit
GCGTTGATACCACTGCTTCCATGTA21100.5314526793023109No Hit
GTACATGGGAAGCAGTGGTATCAAC19240.48460424406523517No Hit
GTATCAACGCAGAGTACATGGAAGC16200.4080347585164662No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15960.4019897991310371No Hit
TATCAACGCAGAGTACTTTTTTTTT15500.39040362697563125No Hit
ACTCTGCGTTGATACCACTGCTTCC14380.3621938165102953No Hit
ACGCAGAGTACATGGAAGCAGTGGT14230.3584157168944021No Hit
CAGTGGTATCAACGCAGAGTACATG13420.3380139789685788No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12480.3143378880423147No Hit
TATCAACGCAGAGTACATGGAAGCA11450.2883949373465147No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10210.2571626471884642No Hit
GTATCAACGCAGAGTACATGGGAAG9290.23399030287765255No Hit
GGTATCAACGCAGAGTACTTTTTTT8600.2166110446445438No Hit
ATACCACTGCTTCCATGTACTCTGC8040.20250613941187584No Hit
GGTATCAACGCAGAGTACATGGAAG7680.19343870033373214No Hit
GTACATGGGGTGGTATCAACGCAAA7590.1911718405641962No Hit
GAGTACATGGGAAGCAGTGGTATCA7510.1891568541023865No Hit
AAGCAGTGGTATCAACGCAGAGTAC7040.17731880863925445No Hit
CCACTGCTTCCATGTACTCTGCGTT6360.16019142371387193No Hit
GCTTCCCATGTACTCTGCGTTGATA6240.15716894402115736No Hit
GTCCTAAAGTGTGTATTTCTCATTT6220.15666519740570492No Hit
GTACTCTGCGTTGATACCACTGCTT6060.15263522448208552No Hit
GCGTTGATACCACTGCTTCCCATGT6000.15112398463572824No Hit
GTGGTATCAACGCAGAGTACATGGA5860.14759775832756122No Hit
GTCCTACAGTGGACATTTCTAAATT5790.14583464517347772No Hit
TATCAACGCAGAGTACATGGGAAGC5490.13827844594169134No Hit
AAAAAGTACTCTGCGTTGATACCAC5220.13147786663308356No Hit
GAAGCAGTGGTATCAACGCAGAGTA5140.12946288017127383No Hit
ACATGGAAGCAGTGGTATCAACGCA5090.12820351363264276No Hit
CTGCTTCCATGTACTCTGCGTTGAT5040.1269441470940117No Hit
GTTGATACCACTGCTTCCATGTACT4980.12543290724765443No Hit
GGTATCAACGCAGAGTACATGGGAA4950.12467728732447579No Hit
CTGTAGGACGTGGAATATGGCAAGA4820.12140293432403501No Hit
GTATTAGAGGCACTGCCTGCCCAGT4780.12039544109313015No Hit
ACGCAGAGTACATGGGAAGCAGTGG4300.1083055223222719No Hit
GCAGAGTACTTTTTTTTTTTTTTTT4150.1045274227063787No Hit
CCCCATGTACTCTGCGTTGATACCA4060.10226056293684276No Hit
TGGAAGCAGTGGTATCAACGCAGAG4040.10175681632139034No Hit
CCATAGGGTCTTCTCGTCTTATTAT4030.10150494301366413No Hit
GTACATGGGTGGTATCAACGCAAAA3990.10049744978275928No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAA250.006022714519.00088111
GTGATCG250.006022714519.0008819
CTAGGAC508.731276E-515.1911283
GATATAC456.776571E-414.7691531
TACAGGT400.005268307414.2506637
GTTCTAG400.00529039114.2416831
TAGGACC1350.014.0694064
AAGTGTA551.9535936E-413.8188247
GTCCTAA1650.013.8101161
AGAACAG903.621608E-813.7193995
ATGGGCT707.245355E-613.5686385
AGGACGT2950.013.5226415
GACGTGG1550.013.4844977
CGTAAAT500.001492749913.30397216
AATCGTA500.001492749913.30397213
CCATGTG500.00149559113.3006179
TAGAGTG500.001498436913.2972635
TAAGACA500.001499861613.2955884
TCCTATA500.001502714413.2922362
CCATTAA500.001502714413.2922361
Produced by FastQC (version 0.11.5)