##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062573_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	397025
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.857537938416975	32.0	32.0	32.0	32.0	32.0
2	30.690757508972986	32.0	32.0	32.0	32.0	32.0
3	30.693963856180343	32.0	32.0	32.0	32.0	32.0
4	30.705265411498015	32.0	32.0	32.0	32.0	32.0
5	30.64868207291732	32.0	32.0	32.0	32.0	32.0
6	34.16479063031295	36.0	36.0	36.0	32.0	36.0
7	33.95421195138845	36.0	36.0	36.0	32.0	36.0
8	33.99197783514892	36.0	36.0	36.0	32.0	36.0
9	34.05178011460235	36.0	36.0	36.0	32.0	36.0
10	33.86771865751527	36.0	36.0	36.0	32.0	36.0
11	34.18412442541402	36.0	36.0	36.0	32.0	36.0
12	33.99495245891317	36.0	36.0	36.0	32.0	36.0
13	34.03154965052578	36.0	36.0	36.0	32.0	36.0
14	33.98333354322776	36.0	36.0	36.0	32.0	36.0
15	33.96843775580883	36.0	36.0	36.0	32.0	36.0
16	33.9133203198791	36.0	36.0	36.0	32.0	36.0
17	33.83953907184686	36.0	36.0	36.0	32.0	36.0
18	33.8733203198791	36.0	36.0	36.0	32.0	36.0
19	33.87505068950318	36.0	36.0	36.0	32.0	36.0
20	33.809462880171274	36.0	36.0	36.0	32.0	36.0
21	33.71216422139664	36.0	36.0	36.0	27.0	36.0
22	33.66637617278509	36.0	36.0	36.0	27.0	36.0
23	33.54677665134437	36.0	36.0	36.0	27.0	36.0
24	33.57913229645488	36.0	36.0	36.0	27.0	36.0
25	33.000234242176184	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	13.0
5	22.0
6	63.0
7	20.0
8	49.0
9	78.0
10	44.0
11	19.0
12	30.0
13	26.0
14	145.0
15	185.0
16	236.0
17	341.0
18	456.0
19	637.0
20	937.0
21	1306.0
22	1830.0
23	2490.0
24	3103.0
25	4302.0
26	5578.0
27	6947.0
28	8867.0
29	11442.0
30	14930.0
31	19957.0
32	28058.0
33	39765.0
34	84205.0
35	160943.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.71190675906124	17.979914003521472	12.549843448121251	22.758335789296034
2	12.07690408902457	22.86594427400545	40.915685592951384	24.1414660440186
3	19.277405834554955	29.557899721420405	28.49949875823019	22.665195685794455
4	10.622514087363061	18.07716625481439	37.67176255141856	33.62855710640399
5	10.646161753331334	41.41386147594057	35.045211419127114	12.89476535160098
6	29.125606919958376	36.095202340248086	21.34216553576513	13.43702520402841
7	26.715862322730562	33.577295398240345	22.46189982937777	17.244942449651322
8	27.995321425150365	33.49970506828803	22.27087335077716	16.234100155784443
9	28.17252932590133	16.209729669770056	19.452532754504453	36.16520824982416
10	14.534112627900292	30.316340475746223	34.970675595943504	20.178871300409988
11	33.55026601780176	23.614312009884266	22.090824276961094	20.744597695352883
12	21.583882259187153	27.409893779064486	31.677140810829897	19.329083150918464
13	30.490049726153135	22.862431033961045	25.596360812160214	21.051158427725607
14	22.838908472900936	21.751617572154057	27.931928327558992	27.477545627386014
15	24.663716702595718	28.320159348486566	28.25989939866117	18.756224550256544
16	23.113249172383714	28.007846281462655	29.011827412063408	19.867077134090227
17	21.13323162659337	27.74727976498178	30.203371452347028	20.916117156077817
18	22.04006183533945	25.503294774510323	33.27835737900877	19.178286011141456
19	21.433687236779058	31.62761960003026	28.11489672912516	18.823796434065518
20	21.640840719680256	25.5713439158776	34.055703352539936	18.73211201190221
21	22.531023752304243	28.438208452380653	25.704890417625982	23.325877377689125
22	20.39609754128959	30.7691919650514	27.34117255364872	21.49353794001029
23	19.872181266535353	29.53576175475852	27.184683946239463	23.407373032466662
24	19.41416051951327	31.602799319084546	28.081205472542713	20.901834688859466
25	23.038823811265082	27.8242778064988	26.903047650270466	22.233850731965653
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	151.0
1	151.0
2	337.5
3	524.0
4	524.0
5	524.0
6	1568.0
7	2612.0
8	2612.0
9	2612.0
10	2629.5
11	2647.0
12	2647.0
13	2647.0
14	2501.0
15	2355.0
16	2355.0
17	2355.0
18	3586.0
19	4817.0
20	4817.0
21	4817.0
22	7527.5
23	10238.0
24	10238.0
25	10238.0
26	14795.5
27	19353.0
28	19353.0
29	19353.0
30	23929.5
31	28506.0
32	28506.0
33	28506.0
34	31161.5
35	33817.0
36	33817.0
37	33817.0
38	35484.0
39	37151.0
40	37151.0
41	37151.0
42	43185.5
43	49220.0
44	49220.0
45	49220.0
46	69620.5
47	90021.0
48	90021.0
49	90021.0
50	71969.0
51	53917.0
52	53917.0
53	53917.0
54	41239.0
55	28561.0
56	28561.0
57	28561.0
58	22407.5
59	16254.0
60	16254.0
61	16254.0
62	12489.5
63	8725.0
64	8725.0
65	8725.0
66	6575.0
67	4425.0
68	4425.0
69	4425.0
70	3243.0
71	2061.0
72	2061.0
73	2061.0
74	1502.0
75	943.0
76	943.0
77	943.0
78	645.0
79	347.0
80	347.0
81	347.0
82	226.0
83	105.0
84	105.0
85	105.0
86	75.0
87	45.0
88	45.0
89	45.0
90	32.5
91	20.0
92	20.0
93	20.0
94	18.0
95	16.0
96	16.0
97	16.0
98	105.0
99	194.0
100	194.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008059945847238839
2	0.0027706063849883508
3	0.0027706063849883508
4	0.009571185693596121
5	0.02820981046533594
6	0.03626975631257477
7	0.060953340469743725
8	0.08135507839556702
9	0.09117813739688937
10	0.10754990239909326
11	0.10956488886090297
12	0.12417354070902335
13	0.11409860839997482
14	0.11157987532271268
15	0.10326805616774762
16	0.10175681632139034
17	0.1158617215540583
18	0.12291417417039228
19	0.12467728732447579
20	0.1158617215540583
21	0.11938794786222531
22	0.14029343240350103
23	0.1324853598639884
24	0.12593665386310687
25	0.12341792078584472
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	397025.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.12961887180265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.51186199090428	43.53441536860422
2	10.948356828582119	11.414673374929677
3	2.5864421443206562	4.044907296522114
4	0.912261873125306	1.9022345502919598
5	0.44854092206858137	1.169113365792104
6	0.26574252472645776	0.83118339192125
7	0.16697591524372266	0.609307357569798
8	0.12737752237167616	0.5312113355255995
9	0.10435018086689131	0.4895761642015246
>10	0.7240580900259974	7.7583510192211005
>50	0.10613541496529252	3.888875381980788
>100	0.07948040207446391	7.78128771292187
>500	0.009208095362285455	3.139294841583349
>1k	0.008238822166255405	8.497014598983805
>5k	9.692731960300478E-4	4.408554239950864
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	9610	2.420502487248914	No Hit
TCCATGTACTCTGCGTTGATACCAC	7840	1.9746867325735156	No Hit
GAGTACATGGAAGCAGTGGTATCAA	4144	1.0437629872174297	No Hit
CATGTACTCTGCGTTGATACCACTG	3466	0.8729928845790568	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2629	0.6621749260122158	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2354	0.592909766387507	No Hit
CCCATGTACTCTGCGTTGATACCAC	2322	0.5848498205402682	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2301	0.5795604810780177	No Hit
GCGTTGATACCACTGCTTCCATGTA	2110	0.5314526793023109	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1924	0.48460424406523517	No Hit
GTATCAACGCAGAGTACATGGAAGC	1620	0.4080347585164662	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1596	0.4019897991310371	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1550	0.39040362697563125	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1438	0.3621938165102953	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1423	0.3584157168944021	No Hit
CAGTGGTATCAACGCAGAGTACATG	1342	0.3380139789685788	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1248	0.3143378880423147	No Hit
TATCAACGCAGAGTACATGGAAGCA	1145	0.2883949373465147	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1021	0.2571626471884642	No Hit
GTATCAACGCAGAGTACATGGGAAG	929	0.23399030287765255	No Hit
GGTATCAACGCAGAGTACTTTTTTT	860	0.2166110446445438	No Hit
ATACCACTGCTTCCATGTACTCTGC	804	0.20250613941187584	No Hit
GGTATCAACGCAGAGTACATGGAAG	768	0.19343870033373214	No Hit
GTACATGGGGTGGTATCAACGCAAA	759	0.1911718405641962	No Hit
GAGTACATGGGAAGCAGTGGTATCA	751	0.1891568541023865	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	704	0.17731880863925445	No Hit
CCACTGCTTCCATGTACTCTGCGTT	636	0.16019142371387193	No Hit
GCTTCCCATGTACTCTGCGTTGATA	624	0.15716894402115736	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	622	0.15666519740570492	No Hit
GTACTCTGCGTTGATACCACTGCTT	606	0.15263522448208552	No Hit
GCGTTGATACCACTGCTTCCCATGT	600	0.15112398463572824	No Hit
GTGGTATCAACGCAGAGTACATGGA	586	0.14759775832756122	No Hit
GTCCTACAGTGGACATTTCTAAATT	579	0.14583464517347772	No Hit
TATCAACGCAGAGTACATGGGAAGC	549	0.13827844594169134	No Hit
AAAAAGTACTCTGCGTTGATACCAC	522	0.13147786663308356	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	514	0.12946288017127383	No Hit
ACATGGAAGCAGTGGTATCAACGCA	509	0.12820351363264276	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	504	0.1269441470940117	No Hit
GTTGATACCACTGCTTCCATGTACT	498	0.12543290724765443	No Hit
GGTATCAACGCAGAGTACATGGGAA	495	0.12467728732447579	No Hit
CTGTAGGACGTGGAATATGGCAAGA	482	0.12140293432403501	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	478	0.12039544109313015	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	430	0.1083055223222719	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	415	0.1045274227063787	No Hit
CCCCATGTACTCTGCGTTGATACCA	406	0.10226056293684276	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	404	0.10175681632139034	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	403	0.10150494301366413	No Hit
GTACATGGGTGGTATCAACGCAAAA	399	0.10049744978275928	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	2.518733077262137E-4	0.0
9	0.0	0.0	0.0	2.518733077262137E-4	0.0
10	0.0	0.0	0.0	2.518733077262137E-4	0.0
11	0.0	0.0	0.0	2.518733077262137E-4	0.0
12	0.0	0.0	0.0	2.518733077262137E-4	0.0
13	0.0	0.0	0.0	2.518733077262137E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGAA	25	0.0060227145	19.000881	11
GTGATCG	25	0.0060227145	19.000881	9
CTAGGAC	50	8.731276E-5	15.191128	3
GATATAC	45	6.776571E-4	14.769153	1
TACAGGT	40	0.0052683074	14.250663	7
GTTCTAG	40	0.005290391	14.241683	1
TAGGACC	135	0.0	14.069406	4
AAGTGTA	55	1.9535936E-4	13.818824	7
GTCCTAA	165	0.0	13.810116	1
AGAACAG	90	3.621608E-8	13.719399	5
ATGGGCT	70	7.245355E-6	13.568638	5
AGGACGT	295	0.0	13.522641	5
GACGTGG	155	0.0	13.484497	7
CGTAAAT	50	0.0014927499	13.303972	16
AATCGTA	50	0.0014927499	13.303972	13
CCATGTG	50	0.001495591	13.300617	9
TAGAGTG	50	0.0014984369	13.297263	5
TAAGACA	50	0.0014998616	13.295588	4
TCCTATA	50	0.0015027144	13.292236	2
CCATTAA	50	0.0015027144	13.292236	1
>>END_MODULE