FastQCFastQC Report
Thu 2 Feb 2017
SRR4062573_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062573_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397025
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA48741.2276305018575657No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC39270.9891064794408412No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA19020.47906303129525846No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC18320.46143189975442356No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG12470.3140860147345885No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC10270.25867388703482147No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA10080.2538882941880234No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG9980.25136956111076125No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8650.21787041118317488No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8590.21635917133681756No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA8110.20426925256595932No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA6810.17152572256155155No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6610.16648825640702725No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC6430.1619545368679554No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG6060.15263522448208552No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA5990.15087211132800202No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA5340.13450034632579813No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG5240.131981613248536No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA5070.12769976701719035No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG5020.12644040047855928No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT4680.11787670801586801No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT4400.11082425539953404No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATA401.8170462E-532.99990544
CGTTCCA502.3562352E-630.7999118
CGATGAT651.7407729E-830.4614543
CGGATTT407.0238777E-427.4999235
CGCAAGG508.3277475E-526.39992316
TAAGGCT609.731679E-625.6665944
ACCGCAA551.5910223E-423.99993114
TGATTCC551.5910223E-423.99993143
GGCGGAT551.5910223E-423.99993133
CCGCAAG551.5910223E-423.99993115
GATGATA1254.0017767E-1122.87993644
TAGGACA1306.730261E-1121.9999374
CCGTTCC703.2084157E-521.99993717
AGTACCG602.8681295E-421.99993711
TGGTATA2200.021.99993744
TACCGCA602.8681295E-421.99993713
CCTACAG4050.021.7283333
TAGAACT1259.895302E-1021.119944
TCCTACA3900.020.8717372
GCGGGGT856.096563E-620.70582444
Produced by FastQC (version 0.11.5)