##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062573_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	397025
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.151496757131163	32.0	32.0	32.0	32.0	32.0
2	31.451304074050753	32.0	32.0	32.0	32.0	32.0
3	31.532739751904792	32.0	32.0	32.0	32.0	32.0
4	31.635715635035577	32.0	32.0	32.0	32.0	32.0
5	31.604717587053713	32.0	32.0	32.0	32.0	32.0
6	35.173160380328696	36.0	36.0	36.0	36.0	36.0
7	35.1837718027832	36.0	36.0	36.0	36.0	36.0
8	35.14810402367609	36.0	36.0	36.0	36.0	36.0
9	35.23594987721176	36.0	36.0	36.0	36.0	36.0
10	35.16355141363894	36.0	36.0	36.0	36.0	36.0
11	35.24259429506958	36.0	36.0	36.0	36.0	36.0
12	35.171309111516905	36.0	36.0	36.0	36.0	36.0
13	35.18257036710535	36.0	36.0	36.0	36.0	36.0
14	35.150003148416346	36.0	36.0	36.0	36.0	36.0
15	35.14038914426044	36.0	36.0	36.0	36.0	36.0
16	35.117302436874255	36.0	36.0	36.0	36.0	36.0
17	35.10337636169007	36.0	36.0	36.0	36.0	36.0
18	35.09202946917701	36.0	36.0	36.0	36.0	36.0
19	35.056719350166865	36.0	36.0	36.0	36.0	36.0
20	35.041899124740254	36.0	36.0	36.0	36.0	36.0
21	34.955471317927085	36.0	36.0	36.0	36.0	36.0
22	34.916380580567974	36.0	36.0	36.0	36.0	36.0
23	34.81143756690385	36.0	36.0	36.0	32.0	36.0
24	34.80970215981361	36.0	36.0	36.0	32.0	36.0
25	34.75854417228134	36.0	36.0	36.0	32.0	36.0
26	34.68459416913293	36.0	36.0	36.0	32.0	36.0
27	34.628781562873876	36.0	36.0	36.0	32.0	36.0
28	34.54681695107361	36.0	36.0	36.0	32.0	36.0
29	34.5054996536742	36.0	36.0	36.0	32.0	36.0
30	34.459074365594105	36.0	36.0	36.0	32.0	36.0
31	34.39870285246521	36.0	36.0	36.0	32.0	36.0
32	34.35186701089352	36.0	36.0	36.0	32.0	36.0
33	34.26845412757383	36.0	36.0	36.0	32.0	36.0
34	34.22727032302752	36.0	36.0	36.0	32.0	36.0
35	34.06162080473522	36.0	36.0	36.0	32.0	36.0
36	34.005891316667714	36.0	36.0	36.0	32.0	36.0
37	33.861509980479816	36.0	36.0	36.0	32.0	36.0
38	33.72400730432592	36.0	36.0	36.0	27.0	36.0
39	33.447133052074804	36.0	36.0	36.0	21.0	36.0
40	33.25134689251306	36.0	36.0	36.0	14.0	36.0
41	33.2983111894717	36.0	36.0	36.0	14.0	36.0
42	32.98495056986336	36.0	36.0	36.0	14.0	36.0
43	33.034612429947735	36.0	36.0	36.0	14.0	36.0
44	33.050576160191426	36.0	36.0	36.0	14.0	36.0
45	32.66464580316101	36.0	36.0	36.0	14.0	36.0
46	32.878506391285185	36.0	36.0	36.0	14.0	36.0
47	32.68310307915119	36.0	36.0	36.0	14.0	36.0
48	32.71728228700963	36.0	36.0	36.0	14.0	36.0
49	32.80291921163655	36.0	36.0	36.0	14.0	36.0
50	32.11322712675524	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	3.0
21	22.0
22	82.0
23	243.0
24	580.0
25	1454.0
26	2894.0
27	5242.0
28	8450.0
29	11860.0
30	15571.0
31	20672.0
32	29026.0
33	33248.0
34	57147.0
35	210529.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.22776108198794	18.468006640250092	13.103661961896654	23.20057031586531
2	11.741236388916176	23.36786638153603	40.298127759684235	24.592769469863555
3	19.047403153493526	29.274343861770184	28.965291421087098	22.712961563649188
4	10.622504880045337	18.345947988161953	37.855802531326745	33.17574460046597
5	10.383980857628613	41.34525533656571	35.25848498205403	13.012278823751652
6	29.440033851857823	36.416438300959136	20.64081768356573	13.502710163617312
7	26.24771739814873	33.43416661419306	22.71267552421132	17.605440463446886
8	27.897991310370884	33.85328379824948	22.33285057616019	15.915874315219444
9	28.045840942006173	15.635287450412442	19.63830993010516	36.680561677476234
10	14.543668534727033	30.160317360367735	35.153453812732195	20.142560292173037
11	33.753038221774446	23.294754738366603	22.159813613752284	20.792393426106667
12	21.486556262200114	27.433033184308293	31.916378061834898	19.164032491656695
13	30.81972167999496	22.327309363390217	25.825829607707323	21.0271393489075
14	22.86102890246206	21.53592343051445	28.01385303192494	27.589194635098547
15	24.868459165039987	28.151375857943457	28.114854228323154	18.865310748693407
16	23.27233801397897	27.650399848876017	29.131918644921605	19.94534349222341
17	20.977772180593163	27.503557710471632	30.636106038662554	20.882564070272654
18	22.418991247402555	24.861658585731377	33.47068824381336	19.248661923052705
19	21.539701530130344	31.00510043448146	28.531452679302312	18.923745356085888
20	21.75228260185127	25.208991877085822	34.37214281216548	18.666582708897426
21	22.635350418739375	28.082866318241923	26.11649140482337	23.165291858195328
22	20.489893583527486	30.251243624456897	27.74938605881242	21.509476733203197
23	19.959196524148354	28.762672375794974	27.527233801397898	23.750897298658774
24	19.40255651407342	31.090233612492916	28.486619230527044	21.020590642906615
25	23.30961526352245	26.693785026131856	27.32422391537057	22.672375794975128
26	18.515458724261695	26.988476796171522	30.13714501605692	24.358919463509853
27	22.904099238083244	29.38278445941691	28.860399219192743	18.8527170833071
28	20.944776777281028	26.096593413513002	28.995403312134	23.963226497071975
29	19.96146338391789	29.391348151879605	30.879919400541528	19.76726906366098
30	23.667527233801398	26.44997166425288	30.294817706693532	19.58768339525219
31	19.70807883634532	27.325231408601475	29.72155405830867	23.245135696744537
32	19.311378376676533	29.155594735847867	30.250739877841447	21.282287009634153
33	19.521188842012467	28.031232290158048	30.121780744285626	22.32579812354386
34	18.222026320760655	30.023802027580125	32.26975631257478	19.48441533908444
35	22.3598010200869	28.369498142434356	29.345003463257978	19.925697374220768
36	20.720861406712423	28.845538694036897	30.285498394307663	20.148101504943014
37	22.276682828537243	28.50802846168377	29.18078206662049	20.034506643158494
38	20.745293117561868	29.431899754423522	27.419683898998805	22.403123229015804
39	20.00402997292362	28.90019520181349	29.97367923934261	21.12209558592028
40	22.612681821044013	28.403752912285118	27.84383854920975	21.139726717461116
41	19.721679994962535	27.895220703985895	30.036647566274166	22.346451734777407
42	22.873622567848372	28.836471254958756	28.914300107046152	19.375606070146716
43	21.471947610351993	26.691770039670047	28.27403815880612	23.562244191171843
44	21.063912851835525	27.472073547005856	31.748126692273786	19.71588690888483
45	22.829544738996287	27.483659719161263	29.049304199987407	20.637491341855046
46	20.8347606190054	27.436628516160233	30.91097767389377	20.817633190940597
47	23.03331024494679	26.144953088596434	30.54920974749701	20.272526918959763
48	19.49373465147031	32.28789119073106	27.49222341162395	20.726150746174675
49	19.71739814873119	27.06051256218122	32.71254958755746	20.50953970153013
50	18.92021912977772	32.23550154272401	26.220515080914303	22.62376424658397
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	16.0
1	15.0
2	14.0
3	94.0
4	174.0
5	190.0
6	206.0
7	196.5
8	187.0
9	276.5
10	366.0
11	500.0
12	634.0
13	1123.5
14	1613.0
15	2192.0
16	2771.0
17	3182.0
18	3593.0
19	3674.0
20	3755.0
21	4219.0
22	4683.0
23	4731.0
24	4779.0
25	5296.5
26	5814.0
27	7103.0
28	8392.0
29	9514.5
30	10637.0
31	11485.0
32	12333.0
33	14271.5
34	16210.0
35	18784.5
36	21359.0
37	23050.0
38	24741.0
39	24684.0
40	24627.0
41	25403.0
42	26179.0
43	27382.0
44	28585.0
45	33563.5
46	38542.0
47	42369.5
48	46197.0
49	40473.5
50	34750.0
51	28438.0
52	22126.0
53	18633.5
54	15141.0
55	13479.0
56	11817.0
57	10434.0
58	9051.0
59	7584.5
60	6118.0
61	5065.0
62	4012.0
63	3304.5
64	2597.0
65	2205.0
66	1813.0
67	1515.0
68	1217.0
69	972.5
70	728.0
71	614.5
72	501.0
73	411.5
74	322.0
75	269.0
76	216.0
77	160.0
78	104.0
79	82.5
80	61.0
81	41.5
82	22.0
83	17.5
84	13.0
85	8.0
86	3.0
87	3.5
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013097412001763113
2	0.0035262263081669923
3	0.0012593665386310686
4	0.0
5	0.0
6	2.518733077262137E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.518733077262137E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	397025.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.27844333928901
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.65253322212996	52.72027993801702
2	10.300950064692435	12.830542698895867
3	2.4477397585449765	4.57324265585605
4	0.8505839988208277	2.118921895034752
5	0.39128110153612206	1.2184188955875985
6	0.24638783385558444	0.9206790450159102
7	0.1620014198147609	0.7062437371372573
8	0.10274854569183038	0.5119215584850406
9	0.08301197127298819	0.4652870714466734
>10	0.6156196824009187	7.707683227030534
>50	0.08396491594337176	3.58784335007439
>100	0.05502555170963175	6.392575285402489
>500	0.0052987568312978725	2.2312963808397686
>1k	0.002853176755314239	4.015064261176635
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	4874	1.2276305018575657	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	3927	0.9891064794408412	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	1902	0.47906303129525846	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	1832	0.46143189975442356	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1247	0.3140860147345885	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1027	0.25867388703482147	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1008	0.2538882941880234	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	998	0.25136956111076125	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	865	0.21787041118317488	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	859	0.21635917133681756	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	811	0.20426925256595932	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	681	0.17152572256155155	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	661	0.16648825640702725	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	643	0.1619545368679554	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	606	0.15263522448208552	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	599	0.15087211132800202	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	534	0.13450034632579813	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	524	0.131981613248536	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	507	0.12769976701719035	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	502	0.12644040047855928	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	468	0.11787670801586801	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT	440	0.11082425539953404	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.518733077262137E-4	0.0
8	0.0	0.0	0.0	5.037466154524274E-4	0.0
9	0.0	0.0	0.0	7.556199231786412E-4	0.0
10	0.0	0.0	0.0	7.556199231786412E-4	0.0
11	0.0	0.0	0.0	7.556199231786412E-4	0.0
12	0.0	0.0	0.0	7.556199231786412E-4	0.0
13	0.0	0.0	0.0	7.556199231786412E-4	0.0
14	0.0	0.0	0.0	7.556199231786412E-4	0.0
15	0.0	0.0	0.0	7.556199231786412E-4	0.0
16	0.0	0.0	0.0	0.0012593665386310686	0.0
17	0.0	0.0	0.0	0.0015112398463572824	0.0
18	0.0	0.0	0.0	0.001763113154083496	0.0
19	0.0	0.0	0.0	0.001763113154083496	0.0
20	0.0	0.0	0.0	0.0020149864618097097	0.0
21	0.0	0.0	0.0	0.0030224796927145648	0.0
22	0.0	0.0	0.0	0.0045337195390718465	0.0
23	0.0	0.0	0.0	0.006296832693155343	0.0
24	0.0	0.0	0.0	0.00881556577041748	0.0
25	0.0	0.0	0.0	0.012593665386310685	0.0
26	0.0	0.0	0.0	0.020905484541275738	0.0
27	0.0	0.0	0.0	0.027202317234431082	0.0
28	0.0	0.0	0.0	0.039795982620741764	0.0
29	0.0	0.0	0.0	0.05566400100749323	0.0
30	0.0	0.0	0.0	0.08714816447326995	0.0
31	0.0	0.0	0.0	0.13298910647944084	0.0
32	0.0	0.0	0.0	0.1982242931805302	0.0
33	0.0	0.0	0.0	0.26345947988161955	0.0
34	0.0	0.0	0.0	0.3516151375857943	0.0
35	0.0	0.0	0.0	0.44002266859769534	0.0
36	0.0	0.0	0.0	0.5770417480007556	0.0
37	0.0	0.0	0.0	0.7704804483344877	0.0
38	0.0	0.0	0.0	0.9787796738240665	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTATA	40	1.8170462E-5	32.999905	44
CGTTCCA	50	2.3562352E-6	30.79991	18
CGATGAT	65	1.7407729E-8	30.46145	43
CGGATTT	40	7.0238777E-4	27.49992	35
CGCAAGG	50	8.3277475E-5	26.399923	16
TAAGGCT	60	9.731679E-6	25.666594	4
ACCGCAA	55	1.5910223E-4	23.999931	14
TGATTCC	55	1.5910223E-4	23.999931	43
GGCGGAT	55	1.5910223E-4	23.999931	33
CCGCAAG	55	1.5910223E-4	23.999931	15
GATGATA	125	4.0017767E-11	22.879936	44
TAGGACA	130	6.730261E-11	21.999937	4
CCGTTCC	70	3.2084157E-5	21.999937	17
AGTACCG	60	2.8681295E-4	21.999937	11
TGGTATA	220	0.0	21.999937	44
TACCGCA	60	2.8681295E-4	21.999937	13
CCTACAG	405	0.0	21.728333	3
TAGAACT	125	9.895302E-10	21.11994	4
TCCTACA	390	0.0	20.871737	2
GCGGGGT	85	6.096563E-6	20.705824	44
>>END_MODULE