FastQCFastQC Report
Thu 2 Feb 2017
SRR4062572_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062572_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499739
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG139332.788055364900478No Hit
TCCATGTACTCTGCGTTGATACCAC114662.294397675586656No Hit
GAGTACATGGAAGCAGTGGTATCAA54921.09897366425274No Hit
CATGTACTCTGCGTTGATACCACTG46840.937289264996328No Hit
GTATCAACGCAGAGTACTTTTTTTT44240.8852621068197598No Hit
GTACTTTTTTTTTTTTTTTTTTTTT43720.8748566751844463No Hit
CCCATGTACTCTGCGTTGATACCAC35580.7119716492008829No Hit
GTACATGGGAAGCAGTGGTATCAAC33720.674752220659184No Hit
GCTTCCATGTACTCTGCGTTGATAC33520.6707501315686789No Hit
TATCAACGCAGAGTACTTTTTTTTT32880.657943446479062No Hit
GCGTTGATACCACTGCTTCCATGTA31250.6253264203914444No Hit
CATGGAAGCAGTGGTATCAACGCAG29950.5993128413031602No Hit
GAGTACTTTTTTTTTTTTTTTTTTT29840.5971116923033823No Hit
GTATCAACGCAGAGTACATGGAAGC24250.4852533022237608No Hit
GTACATGGGGTGGTATCAACGCAAA23850.4772491240427503No Hit
ACTCTGCGTTGATACCACTGCTTCC20790.4160171609580201No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19360.3874022239609076No Hit
ACGCAGAGTACATGGAAGCAGTGGT19020.38059867250704865No Hit
CAGTGGTATCAACGCAGAGTACATG18800.3761963745074929No Hit
GTCCTAAAGTGTGTATTTCTCATTT18470.36959292750815925No Hit
GTCCTACAGTGGACATTTCTAAATT18020.3605882270545225No Hit
CTGTAGGACGTGGAATATGGCAAGA16790.3359753791479152No Hit
GGTATCAACGCAGAGTACTTTTTTT16760.33537506578433945No Hit
GTATCAACGCAGAGTACATGGGAAG16750.33517496132981417No Hit
TATCAACGCAGAGTACATGGAAGCA15900.3181660826951669No Hit
GTACATGGGTGGTATCAACGCAAAA13840.27694456506296283No Hit
GTCCTACAGTGTGCATTTCTCATTT13830.2767444606084376No Hit
CTTTAGGACGTGAAATATGGCGAGG13640.2729424759724576No Hit
GTACATGGGAGTGGTATCAACGCAA11350.22711855588617258No Hit
GAGTACATGGGAAGCAGTGGTATCA10890.21791375097801052No Hit
GGTATCAACGCAGAGTACATGGAAG10790.21591270643275792No Hit
GCGTTGATACCACTGCTTCCCATGT10710.2143118707965558No Hit
GATATACACTGTTCTACAAATCCCG10600.21211072179677792No Hit
ATACCACTGCTTCCATGTACTCTGC10360.20730821488817164No Hit
TATCAACGCAGAGTACATGGGAAGC10270.20550727479744427No Hit
AAGCAGTGGTATCAACGCAGAGTAC9750.19510184316213064No Hit
CTGTAGGACCTGGAATATGGCGAGA9570.19149996298067593No Hit
TTGTAGAACAGTGTATATCAATGAG9270.18549682934491807No Hit
GTACTCTGCGTTGATACCACTGCTT9070.18149474025441278No Hit
GCAGAGTACTTTTTTTTTTTTTTTT9050.1810945313453623No Hit
GCTTCCCATGTACTCTGCGTTGATA8800.17609191998223073No Hit
GTATCAACGCAGAGTACATGGGGTG8730.1746911888005539No Hit
GTGTATATCAATGAGTTACAATGAG8640.17289024870982653No Hit
AAAAAGTACTCTGCGTTGATACCAC8330.16668701061954339No Hit
GTGGTATCAACGCAGAGTACATGGA8200.164085652710715No Hit
GAAGCAGTGGTATCAACGCAGAGTA7890.15788241462043184No Hit
CCACTGCTTCCATGTACTCTGCGTT7880.15768231016590661No Hit
GGTATCAACGCAGAGTACATGGGAA7830.1566817878932803No Hit
GTATTAGAGGCACTGCCTGCCCAGT7680.15368022107540136No Hit
ATTTAGAAATGTCCACTGTAGGACG7540.1508787587120477No Hit
CTGAAGGACCTGGAATATGGCGAGA7490.1498782364394214No Hit
CCATAGGGTCTTCTCGTCTTATTAT7420.14847750525774456No Hit
GTCCTTCAGTGTGCATTTCTCATTT7050.14107364044030984No Hit
ACATGGAAGCAGTGGTATCAACGCA6970.13947280480410773No Hit
ACGCAGAGTACATGGGAAGCAGTGG6850.1370715513498046No Hit
TATCAACGCAGAGTACATGGGGTGG6690.13386988007740042No Hit
CTGCTTCCATGTACTCTGCGTTGAT6580.13166873107762253No Hit
GTGTATATCAATGAGTTACAATGAA6510.13026799989594567No Hit
TGGAAGCAGTGGTATCAACGCAGAG6470.12946758207784465No Hit
CTCTAATACTTGTAATGCTAGAGGT6420.12846705980521833No Hit
GTTGATACCACTGCTTCCATGTACT6180.12366455289661202No Hit
CCCCATGTACTCTGCGTTGATACCA6020.12046288162420785No Hit
GTTCTACAGTGTGGTTTTTATCATT5730.11465985244297525No Hit
CCATTGGGATGTCCTGATCCAACAT5670.11345922571582366No Hit
GCAGAGTACATGGAAGCAGTGGTAT5660.11325912126129839No Hit
TTTCTAAATTTTCCACCTTTTTCAG5600.11205849453414683No Hit
GATACCACTGCTTCCATGTACTCTG5370.10745609208006579No Hit
GAATATGGCAAGAAAACTGAAAATC5180.10365410744408582No Hit
GTATCAACGCAGAGTACATGGGAGT5120.10245348071693423No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCAC307.7074894E-419.0019048
TAAATCC250.00604043618.9923864
GACTAAC350.002179061316.2726651
AACCTTG601.4619436E-615.8333326
GTACATA807.754352E-915.4251311
TAGAAAT2050.014.8233264
GATTAAA1300.014.6036751
TCCAACG1006.511982E-1014.25571418
TCGTTGG400.00526194614.25428612
CGCTATC400.00526194614.25428612
GCTATCG400.00526544814.25285713
CCAACGA801.2792952E-714.25285719
GGTCTCT400.00526544814.25285719
AATCGTA801.2792952E-714.25285713
GCGCTAT400.00526895214.25142911
GTCTTAC400.00530057514.2385831
GTTATAT954.9258233E-913.9887841
GTACAAC551.9693047E-413.807111
GTTTAGA551.9693047E-413.807111
CCACCTT1700.013.414453513
Produced by FastQC (version 0.11.5)