##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062572_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	499739
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.622705052037162	32.0	32.0	32.0	32.0	32.0
2	30.619163203192066	32.0	32.0	32.0	32.0	32.0
3	30.668542979435266	32.0	32.0	32.0	32.0	32.0
4	30.582708173666653	32.0	32.0	32.0	32.0	32.0
5	30.628191916180246	32.0	32.0	32.0	32.0	32.0
6	34.09837134984462	36.0	36.0	36.0	32.0	36.0
7	33.77341772405195	36.0	36.0	36.0	32.0	36.0
8	33.73920786650632	36.0	36.0	36.0	27.0	36.0
9	33.75459790010385	36.0	36.0	36.0	32.0	36.0
10	33.695925272992504	36.0	36.0	36.0	27.0	36.0
11	34.026425794264604	36.0	36.0	36.0	32.0	36.0
12	33.845217203380166	36.0	36.0	36.0	32.0	36.0
13	33.89213369378816	36.0	36.0	36.0	32.0	36.0
14	33.846695975299106	36.0	36.0	36.0	32.0	36.0
15	33.91013108842816	36.0	36.0	36.0	32.0	36.0
16	33.831480032577005	36.0	36.0	36.0	32.0	36.0
17	33.773349688537415	36.0	36.0	36.0	32.0	36.0
18	33.858574175719724	36.0	36.0	36.0	32.0	36.0
19	33.74582532081747	36.0	36.0	36.0	32.0	36.0
20	33.62233285775174	36.0	36.0	36.0	27.0	36.0
21	33.47435961571941	36.0	36.0	36.0	27.0	36.0
22	33.436393797562324	36.0	36.0	36.0	27.0	36.0
23	33.40246808834211	36.0	36.0	36.0	21.0	36.0
24	33.41525076089719	36.0	36.0	36.0	21.0	36.0
25	32.853521538242966	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	0.0
4	22.0
5	40.0
6	85.0
7	24.0
8	81.0
9	95.0
10	67.0
11	24.0
12	40.0
13	27.0
14	266.0
15	284.0
16	390.0
17	525.0
18	797.0
19	1200.0
20	1744.0
21	2392.0
22	3247.0
23	4156.0
24	5047.0
25	6091.0
26	7394.0
27	8874.0
28	11142.0
29	14460.0
30	18074.0
31	24547.0
32	34410.0
33	48105.0
34	102673.0
35	203415.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.840254235592184	18.478108602464708	13.254711883672806	22.4269252782703
2	10.799561357869544	22.898640049307218	43.53763277329086	22.76416581953238
3	19.71099873729541	30.440585846902575	28.846896568285104	21.001518847516916
4	10.465381567255582	18.729136480439323	37.47368294654937	33.33179900575573
5	9.599159285392583	42.056948406145224	35.71255567232147	12.63133663614072
6	28.211765412450074	36.68218171626478	21.45060708887621	13.655445782408936
7	24.6972861379378	33.9047535137235	23.368088442307656	18.02987190603105
8	26.434417199786886	36.42376147193257	22.21498932424258	14.92683200403796
9	27.202349118980234	16.54538498671009	20.241922399754834	36.01034349455484
10	15.185671211195368	30.02334037885542	34.88374888557204	19.907239524377175
11	32.44818432256449	23.10643626346106	24.380265464562985	20.06511394941147
12	22.74538878480328	26.542164691266496	32.96049533618539	17.751951187744837
13	31.498964172800818	23.03944157493819	26.491531060791235	18.97006319146975
14	22.632867567210173	23.066643157966443	27.575104286464196	26.725384988359185
15	23.81873949461984	30.661986701365723	29.100128418568406	16.41914538544603
16	21.09395346600903	27.363060489380203	32.778536827723265	18.764449216887503
17	19.2273785528525	28.259728394369134	32.71930211217657	19.793590940601796
18	20.984789739335685	25.16134978790593	37.206930476825946	16.646929995932442
19	21.327455793217453	31.42173020087161	28.169112858788758	19.081701147122175
20	20.816662626295006	26.38816818898521	35.72222812138746	17.072941063332326
21	21.11344874569207	28.301073976116054	26.266129678608642	24.31934759958323
22	19.64399517417462	32.17110036033364	27.936879486634787	20.24802497885695
23	18.60338822737274	31.198424117573975	28.48551767256754	21.712669982485743
24	19.632237432675044	32.59689984611439	29.033886894075405	18.736975827135165
25	22.527766479052165	28.815624167206	28.272617432720264	20.383991921021575
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	345.0
1	345.0
2	759.5
3	1174.0
4	1174.0
5	1174.0
6	3546.0
7	5918.0
8	5918.0
9	5918.0
10	5405.0
11	4892.0
12	4892.0
13	4892.0
14	3882.0
15	2872.0
16	2872.0
17	2872.0
18	4561.0
19	6250.0
20	6250.0
21	6250.0
22	10399.0
23	14548.0
24	14548.0
25	14548.0
26	22939.5
27	31331.0
28	31331.0
29	31331.0
30	39920.0
31	48509.0
32	48509.0
33	48509.0
34	48607.0
35	48705.0
36	48705.0
37	48705.0
38	47518.0
39	46331.0
40	46331.0
41	46331.0
42	51531.0
43	56731.0
44	56731.0
45	56731.0
46	85780.5
47	114830.0
48	114830.0
49	114830.0
50	87097.5
51	59365.0
52	59365.0
53	59365.0
54	41558.0
55	23751.0
56	23751.0
57	23751.0
58	18756.0
59	13761.0
60	13761.0
61	13761.0
62	11226.0
63	8691.0
64	8691.0
65	8691.0
66	7061.0
67	5431.0
68	5431.0
69	5431.0
70	4364.5
71	3298.0
72	3298.0
73	3298.0
74	2523.0
75	1748.0
76	1748.0
77	1748.0
78	1234.0
79	720.0
80	720.0
81	720.0
82	461.5
83	203.0
84	203.0
85	203.0
86	126.0
87	49.0
88	49.0
89	49.0
90	33.0
91	17.0
92	17.0
93	17.0
94	19.0
95	21.0
96	21.0
97	21.0
98	134.5
99	248.0
100	248.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0090047004536368
2	0.0030015668178789328
3	0.0036018801814547193
4	0.012606580635091518
5	0.032817130542143
6	0.044823397813658725
7	0.06803551453858915
8	0.09544982480855006
9	0.09765097380832795
10	0.12086309053325836
11	0.12286413507851099
12	0.13687144689527933
13	0.12426486626018782
14	0.12666611971449096
15	0.11806162816990469
16	0.11546027026107628
17	0.12666611971449096
18	0.13366977562287516
19	0.1328693578047741
20	0.12206371726040993
21	0.1318688355321478
22	0.15147907207562347
23	0.143474893894613
24	0.13346967116834987
25	0.13326956671382462
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	499739.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.525953440508758
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.31229365969948	21.64615369213192
2	13.75376099156948	8.12185813337932
3	4.943477431199481	4.378826534034051
4	2.1695129277221596	2.562277507700253
5	1.2382500297961327	1.8280256363734593
6	0.7723331803240459	1.36823241136847
7	0.551789443701117	1.1404476596578388
8	0.38578137565593557	0.9112450348666057
9	0.27650693155510453	0.7347717708366561
>10	2.0285325940707732	12.21361479896342
>50	0.2804931192940422	5.7712730101935
>100	0.24051946219658207	14.063702129484703
>500	0.02303566680192617	4.945290817504108
>1k	0.021680627578283457	14.134717793908228
>5k	6.77519611821358E-4	1.0986423352507002
>10k+	0.001355039223642716	5.080920734346784
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	13933	2.788055364900478	No Hit
TCCATGTACTCTGCGTTGATACCAC	11466	2.294397675586656	No Hit
GAGTACATGGAAGCAGTGGTATCAA	5492	1.09897366425274	No Hit
CATGTACTCTGCGTTGATACCACTG	4684	0.937289264996328	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4424	0.8852621068197598	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4372	0.8748566751844463	No Hit
CCCATGTACTCTGCGTTGATACCAC	3558	0.7119716492008829	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3372	0.674752220659184	No Hit
GCTTCCATGTACTCTGCGTTGATAC	3352	0.6707501315686789	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3288	0.657943446479062	No Hit
GCGTTGATACCACTGCTTCCATGTA	3125	0.6253264203914444	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2995	0.5993128413031602	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2984	0.5971116923033823	No Hit
GTATCAACGCAGAGTACATGGAAGC	2425	0.4852533022237608	No Hit
GTACATGGGGTGGTATCAACGCAAA	2385	0.4772491240427503	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2079	0.4160171609580201	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1936	0.3874022239609076	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1902	0.38059867250704865	No Hit
CAGTGGTATCAACGCAGAGTACATG	1880	0.3761963745074929	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1847	0.36959292750815925	No Hit
GTCCTACAGTGGACATTTCTAAATT	1802	0.3605882270545225	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1679	0.3359753791479152	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1676	0.33537506578433945	No Hit
GTATCAACGCAGAGTACATGGGAAG	1675	0.33517496132981417	No Hit
TATCAACGCAGAGTACATGGAAGCA	1590	0.3181660826951669	No Hit
GTACATGGGTGGTATCAACGCAAAA	1384	0.27694456506296283	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1383	0.2767444606084376	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1364	0.2729424759724576	No Hit
GTACATGGGAGTGGTATCAACGCAA	1135	0.22711855588617258	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1089	0.21791375097801052	No Hit
GGTATCAACGCAGAGTACATGGAAG	1079	0.21591270643275792	No Hit
GCGTTGATACCACTGCTTCCCATGT	1071	0.2143118707965558	No Hit
GATATACACTGTTCTACAAATCCCG	1060	0.21211072179677792	No Hit
ATACCACTGCTTCCATGTACTCTGC	1036	0.20730821488817164	No Hit
TATCAACGCAGAGTACATGGGAAGC	1027	0.20550727479744427	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	975	0.19510184316213064	No Hit
CTGTAGGACCTGGAATATGGCGAGA	957	0.19149996298067593	No Hit
TTGTAGAACAGTGTATATCAATGAG	927	0.18549682934491807	No Hit
GTACTCTGCGTTGATACCACTGCTT	907	0.18149474025441278	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	905	0.1810945313453623	No Hit
GCTTCCCATGTACTCTGCGTTGATA	880	0.17609191998223073	No Hit
GTATCAACGCAGAGTACATGGGGTG	873	0.1746911888005539	No Hit
GTGTATATCAATGAGTTACAATGAG	864	0.17289024870982653	No Hit
AAAAAGTACTCTGCGTTGATACCAC	833	0.16668701061954339	No Hit
GTGGTATCAACGCAGAGTACATGGA	820	0.164085652710715	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	789	0.15788241462043184	No Hit
CCACTGCTTCCATGTACTCTGCGTT	788	0.15768231016590661	No Hit
GGTATCAACGCAGAGTACATGGGAA	783	0.1566817878932803	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	768	0.15368022107540136	No Hit
ATTTAGAAATGTCCACTGTAGGACG	754	0.1508787587120477	No Hit
CTGAAGGACCTGGAATATGGCGAGA	749	0.1498782364394214	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	742	0.14847750525774456	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	705	0.14107364044030984	No Hit
ACATGGAAGCAGTGGTATCAACGCA	697	0.13947280480410773	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	685	0.1370715513498046	No Hit
TATCAACGCAGAGTACATGGGGTGG	669	0.13386988007740042	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	658	0.13166873107762253	No Hit
GTGTATATCAATGAGTTACAATGAA	651	0.13026799989594567	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	647	0.12946758207784465	No Hit
CTCTAATACTTGTAATGCTAGAGGT	642	0.12846705980521833	No Hit
GTTGATACCACTGCTTCCATGTACT	618	0.12366455289661202	No Hit
CCCCATGTACTCTGCGTTGATACCA	602	0.12046288162420785	No Hit
GTTCTACAGTGTGGTTTTTATCATT	573	0.11465985244297525	No Hit
CCATTGGGATGTCCTGATCCAACAT	567	0.11345922571582366	No Hit
GCAGAGTACATGGAAGCAGTGGTAT	566	0.11325912126129839	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	560	0.11205849453414683	No Hit
GATACCACTGCTTCCATGTACTCTG	537	0.10745609208006579	No Hit
GAATATGGCAAGAAAACTGAAAATC	518	0.10365410744408582	No Hit
GTATCAACGCAGAGTACATGGGAGT	512	0.10245348071693423	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.0010445452526218E-4	0.0
4	0.0	0.0	0.0	2.0010445452526218E-4	0.0
5	0.0	0.0	0.0	2.0010445452526218E-4	0.0
6	0.0	0.0	0.0	2.0010445452526218E-4	0.0
7	0.0	0.0	0.0	2.0010445452526218E-4	0.0
8	0.0	0.0	0.0	2.0010445452526218E-4	0.0
9	0.0	0.0	0.0	4.0020890905052435E-4	0.0
10	2.0010445452526218E-4	0.0	0.0	6.003133635757866E-4	0.0
11	2.0010445452526218E-4	0.0	0.0	6.003133635757866E-4	0.0
12	2.0010445452526218E-4	0.0	0.0	6.003133635757866E-4	0.0
13	2.0010445452526218E-4	0.0	0.0	6.003133635757866E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGCAC	30	7.7074894E-4	19.001904	8
TAAATCC	25	0.006040436	18.992386	4
GACTAAC	35	0.0021790613	16.272665	1
AACCTTG	60	1.4619436E-6	15.833332	6
GTACATA	80	7.754352E-9	15.425131	1
TAGAAAT	205	0.0	14.823326	4
GATTAAA	130	0.0	14.603675	1
TCCAACG	100	6.511982E-10	14.255714	18
TCGTTGG	40	0.005261946	14.254286	12
CGCTATC	40	0.005261946	14.254286	12
GCTATCG	40	0.005265448	14.252857	13
CCAACGA	80	1.2792952E-7	14.252857	19
GGTCTCT	40	0.005265448	14.252857	19
AATCGTA	80	1.2792952E-7	14.252857	13
GCGCTAT	40	0.005268952	14.251429	11
GTCTTAC	40	0.005300575	14.238583	1
GTTATAT	95	4.9258233E-9	13.988784	1
GTACAAC	55	1.9693047E-4	13.80711	1
GTTTAGA	55	1.9693047E-4	13.80711	1
CCACCTT	170	0.0	13.4144535	13
>>END_MODULE