##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062572_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	499739
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.853495524663874	32.0	32.0	32.0	32.0	32.0
2	31.41383802344824	32.0	32.0	32.0	32.0	32.0
3	31.524901998843397	32.0	32.0	32.0	32.0	32.0
4	31.640766480102613	32.0	32.0	32.0	32.0	32.0
5	31.59173888769938	32.0	32.0	32.0	32.0	32.0
6	35.16540634211058	36.0	36.0	36.0	36.0	36.0
7	35.17093722923366	36.0	36.0	36.0	36.0	36.0
8	35.147356920312404	36.0	36.0	36.0	36.0	36.0
9	35.174268968401506	36.0	36.0	36.0	36.0	36.0
10	35.148591564796824	36.0	36.0	36.0	36.0	36.0
11	35.21302519915396	36.0	36.0	36.0	36.0	36.0
12	35.12002265182425	36.0	36.0	36.0	36.0	36.0
13	35.13176678226034	36.0	36.0	36.0	36.0	36.0
14	35.09661443273389	36.0	36.0	36.0	36.0	36.0
15	35.104674640162166	36.0	36.0	36.0	36.0	36.0
16	35.078362905436634	36.0	36.0	36.0	36.0	36.0
17	35.08910251151101	36.0	36.0	36.0	36.0	36.0
18	35.0768401105377	36.0	36.0	36.0	36.0	36.0
19	34.98338932922986	36.0	36.0	36.0	36.0	36.0
20	34.917152753737454	36.0	36.0	36.0	36.0	36.0
21	34.8102989760655	36.0	36.0	36.0	32.0	36.0
22	34.797098085200474	36.0	36.0	36.0	32.0	36.0
23	34.71501323690967	36.0	36.0	36.0	32.0	36.0
24	34.69298974064461	36.0	36.0	36.0	32.0	36.0
25	34.668166783060755	36.0	36.0	36.0	32.0	36.0
26	34.59350781107738	36.0	36.0	36.0	32.0	36.0
27	34.534222864335185	36.0	36.0	36.0	32.0	36.0
28	34.42253456304191	36.0	36.0	36.0	32.0	36.0
29	34.36292744812792	36.0	36.0	36.0	32.0	36.0
30	34.29366729432764	36.0	36.0	36.0	32.0	36.0
31	34.230832494562165	36.0	36.0	36.0	32.0	36.0
32	34.14920188338313	36.0	36.0	36.0	32.0	36.0
33	34.0577461434869	36.0	36.0	36.0	32.0	36.0
34	33.938053664012614	36.0	36.0	36.0	32.0	36.0
35	33.7754667936663	36.0	36.0	36.0	32.0	36.0
36	33.68149574077668	36.0	36.0	36.0	27.0	36.0
37	33.47112792877882	36.0	36.0	36.0	21.0	36.0
38	33.344980079601555	36.0	36.0	36.0	21.0	36.0
39	32.95576691032719	36.0	36.0	36.0	14.0	36.0
40	32.76386073530383	36.0	36.0	36.0	14.0	36.0
41	32.81056311394548	36.0	36.0	36.0	14.0	36.0
42	32.43300402810267	36.0	36.0	36.0	14.0	36.0
43	32.505647948228976	36.0	36.0	36.0	14.0	36.0
44	32.47266473098958	36.0	36.0	36.0	14.0	36.0
45	32.03334740734663	36.0	36.0	36.0	14.0	36.0
46	32.19377114853954	36.0	36.0	36.0	14.0	36.0
47	31.90866832486558	36.0	36.0	36.0	14.0	36.0
48	31.9091645839128	36.0	36.0	36.0	14.0	36.0
49	31.957699919357903	36.0	36.0	36.0	14.0	36.0
50	31.30989176350055	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	4.0
21	22.0
22	97.0
23	297.0
24	845.0
25	1994.0
26	4461.0
27	8566.0
28	14020.0
29	18791.0
30	23474.0
31	29175.0
32	39920.0
33	43645.0
34	72937.0
35	241488.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.18993295306714	19.102571800260183	13.807064945461821	22.900430301210847
2	10.645489219833745	23.322740607627562	42.6976230403791	23.334147132159593
3	19.428897315577256	30.063133460734186	29.561067365703824	20.94690185798473
4	10.577763903813405	18.958171998471197	37.45530148858299	33.008762609132404
5	9.44553056695595	41.692363413701955	36.07603168854142	12.786074330800679
6	28.65155723467779	36.84935125227122	20.546212770044743	13.95287874300625
7	24.180021971469106	33.653166953149544	23.900676152951842	18.266134922429508
8	26.17386275635882	36.460032136775396	22.457722931370174	14.908382175495607
9	26.84801466365443	15.866482303762563	20.677993912822494	36.607509119760515
10	15.28798032573003	29.58644412383264	35.248399664624934	19.877175885812395
11	32.506968637628844	22.52095593900016	24.754922069320184	20.217153354050815
12	22.409697862284112	26.54005390814005	33.4824778534395	17.567770376136345
13	31.89044681323651	22.63281432907978	26.833407038474082	18.643331819209628
14	22.398091803921645	22.768685253702433	28.00181694844709	26.83140599392883
15	23.892872079225356	30.28120678994435	29.399746667760574	16.426174463069724
16	21.115822459323766	26.792385625296404	33.1244909842938	18.967300931086026
17	19.061149920258373	27.82192304382888	33.267765773733885	19.849161262178857
18	21.11061974350611	24.189226776377268	37.950610218534074	16.74954326158255
19	21.553651005825042	30.49331751174113	29.150016308513045	18.803015173920787
20	20.899109335072907	25.63238010241346	36.55308070812964	16.91542985438399
21	20.815665777535873	27.483346306772134	27.503156647770137	24.197831267921856
22	19.592427247022943	31.160865972037406	28.88147613054014	20.365230650399507
23	18.60231040603195	29.797354218902267	29.63166773055535	21.968667644510433
24	19.76911948036875	31.627909768899364	29.905810833254957	18.697159917476924
25	22.755878568612818	27.268434122612	29.12620387842454	20.84948343035064
26	16.126618094645405	28.22813508651516	33.369819045541774	22.27542777329766
27	20.984754041609722	30.50832534583052	31.607299010083263	16.899621602476493
28	18.85104024300685	27.296248641791017	30.83869780025173	23.014013314950404
29	18.471241988317903	28.8494594178161	32.532582007808074	20.146716586057924
30	21.818389199161963	26.92105278955615	32.257238278381315	19.003319732900575
31	18.798412771466705	27.27023506270273	31.36297147110792	22.568380694722645
32	17.42649663124151	30.897328405427633	31.725560742707692	19.950614220623166
33	17.77687953111524	29.886000492256958	32.28925499110536	20.04786498552244
34	16.892417842113584	31.33915904101941	34.48360043942939	17.28482267743762
35	20.31440411895009	30.46410226138044	31.308743163931574	17.912750455737893
36	18.595706959032615	30.494318034013755	32.10035638603351	18.80961862092012
37	20.62476612791877	30.06429356123897	30.131328553504925	19.17961175733733
38	20.00604315452666	31.86883553214778	27.36148269396625	20.763638619359305
39	19.369911093590854	29.256071669411433	31.05841249132047	20.315604745677245
40	22.36407404665235	29.815963933173116	28.83525200154481	18.984710018629723
41	18.958096126177864	28.145491946796223	32.492561116903026	20.403850810122883
42	21.93585051396829	31.770584244975876	29.80795975499211	16.485605486063726
43	19.17040695242917	29.674890292732808	29.046962514432533	22.10774024040549
44	18.677549680933446	29.890002581347463	32.71447695697154	18.71797078074755
45	20.363229605854254	30.020470685697937	29.823367797990553	19.79293191045726
46	19.26109281263382	27.955248550240324	31.671195706550233	21.11246293057562
47	22.7264632137976	27.61081284430473	30.87711785552058	18.785606086377086
48	17.983187223730788	34.25908324145204	26.582075843590353	21.17565369122682
49	18.78760713092234	28.5246898881216	33.460666467896246	19.22703651305982
50	16.83438755030126	35.312032881163965	25.577551481873538	22.27602808666124
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	34.0
1	36.5
2	39.0
3	136.5
4	234.0
5	265.0
6	296.0
7	304.0
8	312.0
9	427.5
10	543.0
11	796.0
12	1049.0
13	1872.5
14	2696.0
15	3871.5
16	5047.0
17	6198.5
18	7350.0
19	7893.5
20	8437.0
21	9127.5
22	9818.0
23	9747.0
24	9676.0
25	9228.0
26	8780.0
27	11049.5
28	13319.0
29	15703.0
30	18087.0
31	19768.0
32	21449.0
33	25433.0
34	29417.0
35	33660.0
36	37903.0
37	38484.0
38	39065.0
39	35178.5
40	31292.0
41	30231.0
42	29170.0
43	28914.5
44	28659.0
45	34323.0
46	39987.0
47	44558.0
48	49129.0
49	41870.5
50	34612.0
51	27220.0
52	19828.0
53	15680.5
54	11533.0
55	10576.5
56	9620.0
57	8923.5
58	8227.0
59	7230.5
60	6234.0
61	5596.5
62	4959.0
63	4230.0
64	3501.0
65	3282.5
66	3064.0
67	2625.5
68	2187.0
69	1901.0
70	1615.0
71	1311.5
72	1008.0
73	845.0
74	682.0
75	584.0
76	486.0
77	350.5
78	215.0
79	162.0
80	109.0
81	73.5
82	38.0
83	24.5
84	11.0
85	10.0
86	9.0
87	6.5
88	4.0
89	4.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	1.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017809296452748334
2	0.005002611363131554
3	8.004178181010487E-4
4	4.0020890905052435E-4
5	0.0
6	0.0014007311816768351
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.0010445452526218E-4
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	499739.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.78520493468914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.5539988381871	32.75378528861789
2	12.386550074290973	10.599221667246567
3	4.267929059253334	5.478126583406069
4	1.9915345121908563	3.4083284895436785
5	1.1235585695154464	2.4035841826422275
6	0.6671766536866243	1.7127173913373408
7	0.47559205629552304	1.4243812515739915
8	0.335577877348212	1.148621460311298
9	0.25389951515281717	0.9776828509768345
>10	1.5968841984066118	13.341274469358892
>50	0.18964953144455982	5.737923140634379
>100	0.13835474502392867	11.76397064136387
>500	0.01364723675406099	3.9628676838332217
>1k	0.004705943708296893	3.0455409300711977
>5k	9.411887416593785E-4	2.241973969082559
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	5916	1.183817952971451	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	5219	1.0443451481673434	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2135	0.42722301041143473	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	2032	0.4066122515953328	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1977	0.39560650659644336	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1399	0.27994613188084183	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1330	0.2661389245185987	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1322	0.2645380888823966	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1298	0.2597355819737903	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1247	0.24953025479300195	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1215	0.24312691224819355	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1171	0.234322316249082	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	981	0.19630246988928218	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	952	0.19049944070804958	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	922	0.18449630707229173	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA	866	0.17329045761887704	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	848	0.16968857743742233	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	752	0.15047854980299716	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	731	0.14627635625796667	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	729	0.14587614734891613	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGACTGTCTCTTATA	721	0.14427531171271404	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG	716	0.14327478944008773	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC	711	0.14227426716746142	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	708	0.14167395380388562	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA	683	0.13667134244075407	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	679	0.13587092462265304	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	664	0.1328693578047741	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	652	0.13046810435047093	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	642	0.12846705980521833	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	630	0.12606580635091516	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	629	0.1258657018963899	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	608	0.1216635083513594	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	596	0.11926225489705625	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	562	0.11245870344319736	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	557	0.11145818117057103	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	544	0.10885682326174262	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT	537	0.10745609208006579	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	524	0.10485473417123738	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	524	0.10485473417123738	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	512	0.10245348071693423	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	502	0.10045243617168162	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.0010445452526218E-4	0.0
4	0.0	0.0	0.0	2.0010445452526218E-4	0.0
5	0.0	0.0	0.0	2.0010445452526218E-4	0.0
6	0.0	0.0	0.0	2.0010445452526218E-4	0.0
7	0.0	0.0	0.0	2.0010445452526218E-4	0.0
8	0.0	0.0	0.0	2.0010445452526218E-4	0.0
9	0.0	0.0	0.0	2.0010445452526218E-4	0.0
10	0.0	0.0	0.0	2.0010445452526218E-4	0.0
11	0.0	0.0	0.0	2.0010445452526218E-4	0.0
12	0.0	0.0	0.0	2.0010445452526218E-4	0.0
13	0.0	0.0	0.0	2.0010445452526218E-4	0.0
14	0.0	0.0	0.0	2.0010445452526218E-4	0.0
15	0.0	0.0	0.0	4.0020890905052435E-4	0.0
16	0.0	0.0	0.0	0.0010005222726263108	0.0
17	0.0	0.0	0.0	0.0010005222726263108	0.0
18	0.0	0.0	0.0	0.0020010445452526217	0.0
19	0.0	0.0	0.0	0.003201671272404195	0.0
20	0.0	0.0	0.0	0.00460240245408103	0.0
21	0.0	0.0	0.0	0.006203238090283128	0.0
22	0.0	0.0	0.0	0.008804595999111537	0.0
23	0.0	0.0	0.0	0.01720898308917255	0.0
24	0.0	0.0	0.0	0.028614936997112494	0.0
25	0.0	0.0	0.0	0.03501827954192088	0.0
26	0.0	0.0	0.0	0.0444231889046082	0.0
27	0.0	0.0	0.0	0.05402820272182079	0.0
28	0.0	0.0	0.0	0.07063687244741755	0.0
29	0.0	0.0	0.0	0.0952497203540248	0.0
30	0.0	0.0	0.0	0.14107364044030984	0.0
31	0.0	0.0	0.0	0.22511751134091995	0.0
32	0.0	0.0	0.0	0.32997224551215737	0.0
33	0.0	0.0	0.0	0.4204194589575759	0.0
34	0.0	0.0	0.0	0.5444842207632384	0.0
35	0.0	0.0	0.0	0.6741519072956083	0.0
36	0.0	0.0	0.0	0.8546461252773948	0.0
37	0.0	0.0	0.0	1.1327913170675092	0.0
38	0.0	0.0	0.0	1.4015315994949364	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCGTT	20	7.852382E-4	43.999798	26
GACGTGT	25	0.0023515287	35.19984	22
TCGGTTA	25	0.0023515287	35.19984	42
CTGGACC	25	0.0023515287	35.19984	4
GATCATA	45	1.0356362E-6	34.222065	44
TCGTATA	95	0.0	32.420906	44
CACGATT	30	0.0057419576	29.3332	28
AGGCCGT	30	0.0057419576	29.3332	13
GCGGGGT	165	0.0	29.3332	44
CGTCTCG	55	4.953583E-6	27.999874	25
CGCGTCT	55	4.953583E-6	27.999874	23
TGTTGTA	40	7.0224E-4	27.502625	2
TCATAAG	40	7.0224E-4	27.502625	2
CGGGCTT	40	7.026509E-4	27.499872	31
GTACTCG	85	8.210918E-9	25.882236	35
GCGTCTC	60	9.738387E-6	25.666552	24
TATGTCG	70	1.1300926E-6	25.142742	16
ACAGGCG	185	0.0	24.972858	40
ACTGATA	45	0.0013971048	24.444332	3
GAATCTC	45	0.0013971048	24.444332	37
>>END_MODULE