FastQCFastQC Report
Thu 2 Feb 2017
SRR4062571_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062571_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2143610
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36190.16882735199033405No Hit
GAATAACGCCGCCGCATCGCCAGTC21770.10155765274466903No Hit
GCGCAAGACGGACCAGAGCGAAAGC21650.10099784942223633No Hit
TATCAACGCAGAGTACTTTTTTTTT21630.10090454886849753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA852.6959788E-713.41366217
CGAACGA1501.8189894E-1212.66816216
TATGCCG604.0922972E-412.6669785
CGTTATT1950.012.65840152
TCGACGC1001.4411853E-712.35145914
ATCGCCA4250.012.29556816
ACGAACG1650.012.09233715
CGGTTCT3550.012.04367412
CCATACG802.8716238E-511.873354
ACGCGCG1052.717352E-711.76329313
CTAAGGT1052.7264286E-711.760274
GCGTTAT2050.011.5756441
CGACGAC1406.7848305E-1011.53707714
CGCCAGT4450.011.52998918
TAACGAA1657.2759576E-1211.51651213
CTCGACG1001.9277686E-611.40134613
GACGCTT1001.9277686E-611.40134616
ATAACGA1751.8189894E-1211.40134612
CGCATCG4500.011.40134513
CGAGACT1001.9337385E-611.3984164
Produced by FastQC (version 0.11.5)