##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062571_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2143610 Sequences flagged as poor quality 0 Sequence length 25 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.206258601144796 32.0 32.0 32.0 32.0 32.0 2 30.817431342455016 32.0 32.0 32.0 32.0 32.0 3 30.785824380367696 32.0 32.0 32.0 32.0 32.0 4 30.872862600939538 32.0 32.0 32.0 32.0 32.0 5 30.629247391083265 32.0 32.0 32.0 32.0 32.0 6 34.3074029324364 36.0 36.0 36.0 32.0 36.0 7 34.18265822607657 36.0 36.0 36.0 32.0 36.0 8 34.19497716468947 36.0 36.0 36.0 32.0 36.0 9 34.36647851054996 36.0 36.0 36.0 32.0 36.0 10 33.99202000363872 36.0 36.0 36.0 32.0 36.0 11 34.35517281595066 36.0 36.0 36.0 32.0 36.0 12 34.09229010874179 36.0 36.0 36.0 32.0 36.0 13 34.235756970717624 36.0 36.0 36.0 32.0 36.0 14 34.13072387234618 36.0 36.0 36.0 32.0 36.0 15 34.0482713739906 36.0 36.0 36.0 32.0 36.0 16 34.05669501448491 36.0 36.0 36.0 32.0 36.0 17 33.9879343723905 36.0 36.0 36.0 32.0 36.0 18 34.001491409351516 36.0 36.0 36.0 32.0 36.0 19 33.97844757208634 36.0 36.0 36.0 32.0 36.0 20 33.979725323169795 36.0 36.0 36.0 32.0 36.0 21 33.96562387747771 36.0 36.0 36.0 32.0 36.0 22 33.91330372595761 36.0 36.0 36.0 32.0 36.0 23 33.877539757698464 36.0 36.0 36.0 32.0 36.0 24 33.846425889037654 36.0 36.0 36.0 32.0 36.0 25 33.38677697902137 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 16.0 4 79.0 5 193.0 6 355.0 7 93.0 8 343.0 9 379.0 10 272.0 11 104.0 12 170.0 13 118.0 14 269.0 15 380.0 16 594.0 17 810.0 18 1102.0 19 1574.0 20 2540.0 21 3831.0 22 5854.0 23 9036.0 24 13047.0 25 18845.0 26 26247.0 27 33890.0 28 46375.0 29 61654.0 30 79124.0 31 108248.0 32 155059.0 33 224143.0 34 491272.0 35 857593.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.333702213702104 17.38382951008737 10.794416221156533 26.488052055053995 2 17.443799768700483 19.870298725333456 36.14099287114173 26.54490863482434 3 19.318594268279597 23.487177573232685 28.064762935558967 29.129465222928747 4 13.139407444525784 15.080112705075788 35.24310488579957 36.53737496459887 5 15.134387213439886 35.895187540103834 33.29722918975675 15.67319605669953 6 34.577734632060896 34.719944366916984 16.729176098310006 13.973144902712113 7 30.45477652831961 30.18313646937012 20.669805846679985 18.69228115563029 8 28.42144681137177 32.260566310047814 19.60051155886779 19.71747531971262 9 27.706272761530837 14.177792450794936 18.508407903443246 39.607526884230985 10 16.3605129687762 26.237268376344623 31.167052073607664 26.235166581271514 11 37.65471107754435 20.903948469108226 22.104131373694717 19.337209079652705 12 25.065667005336977 23.42813766364199 28.29293814011608 23.21325719090495 13 29.709032074851283 19.21109467013422 25.053993662750724 26.025879592263774 14 23.688578955235034 19.58378484418205 24.55024848666019 32.17738771392273 15 25.377072057366796 26.90614332637919 21.930216624656904 25.786567991597114 16 26.06517051555145 25.40053792693105 23.4234532966122 25.110838260905304 17 24.449288589500586 25.417032935723167 24.905859900100797 25.22781857467545 18 25.31226235830249 24.343478317735006 25.941831851975028 24.402427471987473 19 25.94613506779362 24.797618791813385 24.874273739911303 24.381972400481697 20 25.873283512377395 24.12620270901448 24.7478748248482 25.252638953759927 21 26.84416006636734 23.93932769435149 24.00294841396629 25.213563825314882 22 26.121648417161445 24.37201331382234 24.299547920602972 25.20679034841324 23 24.69384894959848 24.086685570935387 25.128024254768498 26.091441224697633 24 25.11984140108168 24.829708895870876 24.796542887385986 25.25390681566146 25 25.06142609436543 24.397930851501133 24.959687665827097 25.580955388306343 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 187.0 1 187.0 2 523.5 3 860.0 4 860.0 5 860.0 6 2345.5 7 3831.0 8 3831.0 9 3831.0 10 4258.5 11 4686.0 12 4686.0 13 4686.0 14 5368.0 15 6050.0 16 6050.0 17 6050.0 18 9758.0 19 13466.0 20 13466.0 21 13466.0 22 22038.0 23 30610.0 24 30610.0 25 30610.0 26 45323.5 27 60037.0 28 60037.0 29 60037.0 30 74162.0 31 88287.0 32 88287.0 33 88287.0 34 109354.0 35 130421.0 36 130421.0 37 130421.0 38 148240.0 39 166059.0 40 166059.0 41 166059.0 42 189349.5 43 212640.0 44 212640.0 45 212640.0 46 242659.5 47 272679.0 48 272679.0 49 272679.0 50 276410.5 51 280142.0 52 280142.0 53 280142.0 54 263432.5 55 246723.0 56 246723.0 57 246723.0 58 228302.0 59 209881.0 60 209881.0 61 209881.0 62 188175.0 63 166469.0 64 166469.0 65 166469.0 66 140261.0 67 114053.0 68 114053.0 69 114053.0 70 87598.5 71 61144.0 72 61144.0 73 61144.0 74 48319.5 75 35495.0 76 35495.0 77 35495.0 78 28754.0 79 22013.0 80 22013.0 81 22013.0 82 16039.5 83 10066.0 84 10066.0 85 10066.0 86 7398.5 87 4731.0 88 4731.0 89 4731.0 90 3274.5 91 1818.0 92 1818.0 93 1818.0 94 1086.5 95 355.0 96 355.0 97 355.0 98 631.0 99 907.0 100 907.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008723601774576532 2 0.003218869103988132 3 0.004665027686939322 4 0.01478813776759765 5 0.034287953499004015 6 0.04665027686939322 7 0.06703644786131806 8 0.08961518186610437 9 0.09857203502502787 10 0.12049766515364267 11 0.12143067069103056 12 0.13458604876819943 13 0.12213042484407145 14 0.12362323370389203 15 0.11387332583818885 16 0.11322022196201735 17 0.12371653425763082 18 0.12978107025065194 19 0.13285998852403189 20 0.1217572226291163 21 0.13052747468056222 22 0.152919607577871 23 0.13995083060817967 24 0.13360639295394217 25 0.13183368243290522 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2143610.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.373484474936426 #Duplication Level Percentage of deduplicated Percentage of total 1 79.9316086407866 41.063652555641454 2 12.025099073612447 12.355424811356029 3 3.3563209985698097 5.172777141387877 4 1.4280400567784604 2.9345357474598224 5 0.7805403020015667 2.004953754346984 6 0.49098809695878876 1.5134261625894552 7 0.32799542754872807 1.1795187603517303 8 0.23751193961535416 0.9761452753953148 9 0.18728125550097333 0.8659161604733271 >10 1.020485725357965 9.731489520629037 >50 0.1016060529917252 3.670061786254629 >100 0.09421167286053829 10.21146076387242 >500 0.013755842636779172 4.7619843207839665 >1k 0.004554914780390453 3.5586532394580104 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3619 0.16882735199033405 No Hit GAATAACGCCGCCGCATCGCCAGTC 2177 0.10155765274466903 No Hit GCGCAAGACGGACCAGAGCGAAAGC 2165 0.10099784942223633 No Hit TATCAACGCAGAGTACTTTTTTTTT 2163 0.10090454886849753 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.330055373878644E-5 2 0.0 0.0 0.0 0.0 9.330055373878644E-5 3 0.0 0.0 0.0 0.0 9.330055373878644E-5 4 0.0 0.0 0.0 0.0 9.330055373878644E-5 5 0.0 0.0 0.0 0.0 9.330055373878644E-5 6 0.0 0.0 0.0 0.0 9.330055373878644E-5 7 0.0 0.0 0.0 0.0 9.330055373878644E-5 8 0.0 0.0 0.0 0.0 9.330055373878644E-5 9 0.0 0.0 0.0 0.0 9.330055373878644E-5 10 0.0 0.0 0.0 0.0 9.330055373878644E-5 11 0.0 0.0 0.0 0.0 9.330055373878644E-5 12 0.0 0.0 0.0 0.0 1.8660110747757287E-4 13 0.0 0.0 0.0 0.0 2.332513843469661E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCTTA 85 2.6959788E-7 13.413662 17 CGAACGA 150 1.8189894E-12 12.668162 16 TATGCCG 60 4.0922972E-4 12.666978 5 CGTTATT 195 0.0 12.6584015 2 TCGACGC 100 1.4411853E-7 12.351459 14 ATCGCCA 425 0.0 12.295568 16 ACGAACG 165 0.0 12.092337 15 CGGTTCT 355 0.0 12.043674 12 CCATACG 80 2.8716238E-5 11.87335 4 ACGCGCG 105 2.717352E-7 11.763293 13 CTAAGGT 105 2.7264286E-7 11.76027 4 GCGTTAT 205 0.0 11.575644 1 CGACGAC 140 6.7848305E-10 11.537077 14 CGCCAGT 445 0.0 11.529989 18 TAACGAA 165 7.2759576E-12 11.516512 13 CTCGACG 100 1.9277686E-6 11.401346 13 GACGCTT 100 1.9277686E-6 11.401346 16 ATAACGA 175 1.8189894E-12 11.401346 12 CGCATCG 450 0.0 11.401345 13 CGAGACT 100 1.9337385E-6 11.398416 4 >>END_MODULE