FastQCFastQC Report
Thu 2 Feb 2017
SRR4062571_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062571_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2143610
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC22250.10379686603439991No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA21690.1011844505297139No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA654.934034E-420.3075949
TAGCGGC8100.017.9258430
TAGGACG6550.017.8014434
CGCAATA7950.017.71060836
ATACGAA7700.017.42848840
TGTAGGA8150.017.2767982
TATACCG902.216458E-417.111035
GTATCAA36450.016.9629081
CTAGCGG8200.016.90235729
CAATACG8250.016.7999238
CGTCGTA6950.016.14380810
TCTAGCG8450.016.14193328
CCTAATA1501.8386527E-716.1340082
CGAATGC8300.015.90353843
AATACGA8600.015.8603939
GTCCTAA5000.015.8425111
ATACCGT7500.015.8399266
GCATTCG8650.015.76871122
ACCGTCG7400.015.7566818
TAATACT5450.015.7430434
Produced by FastQC (version 0.11.5)