##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062571_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2143610
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.296802123520603	32.0	32.0	32.0	32.0	32.0
2	31.436074659103102	32.0	32.0	32.0	32.0	32.0
3	31.508178260037973	32.0	32.0	32.0	32.0	32.0
4	31.60409309529252	32.0	32.0	32.0	32.0	32.0
5	31.55028060141537	32.0	32.0	32.0	32.0	32.0
6	35.14077980602815	36.0	36.0	36.0	36.0	36.0
7	35.154064405372246	36.0	36.0	36.0	36.0	36.0
8	35.09430726671363	36.0	36.0	36.0	36.0	36.0
9	35.22061802286797	36.0	36.0	36.0	36.0	36.0
10	35.09644618190809	36.0	36.0	36.0	36.0	36.0
11	35.22989256441237	36.0	36.0	36.0	36.0	36.0
12	35.1447963948666	36.0	36.0	36.0	36.0	36.0
13	35.18811724147583	36.0	36.0	36.0	36.0	36.0
14	35.144060720000375	36.0	36.0	36.0	36.0	36.0
15	35.108191788618264	36.0	36.0	36.0	36.0	36.0
16	35.1256973050135	36.0	36.0	36.0	36.0	36.0
17	35.09143967419447	36.0	36.0	36.0	36.0	36.0
18	35.109052019723734	36.0	36.0	36.0	36.0	36.0
19	35.088593074299894	36.0	36.0	36.0	36.0	36.0
20	35.0821586016113	36.0	36.0	36.0	36.0	36.0
21	35.08564151128237	36.0	36.0	36.0	36.0	36.0
22	35.06896123828495	36.0	36.0	36.0	36.0	36.0
23	35.02964158592281	36.0	36.0	36.0	36.0	36.0
24	35.003706831000045	36.0	36.0	36.0	36.0	36.0
25	34.98470430722006	36.0	36.0	36.0	36.0	36.0
26	34.9429546419358	36.0	36.0	36.0	32.0	36.0
27	34.92261138919859	36.0	36.0	36.0	32.0	36.0
28	34.90914578678024	36.0	36.0	36.0	32.0	36.0
29	34.8861863865162	36.0	36.0	36.0	32.0	36.0
30	34.87309351981004	36.0	36.0	36.0	32.0	36.0
31	34.87831975032772	36.0	36.0	36.0	32.0	36.0
32	34.84055588469918	36.0	36.0	36.0	32.0	36.0
33	34.82616334127943	36.0	36.0	36.0	32.0	36.0
34	34.81125297978644	36.0	36.0	36.0	32.0	36.0
35	34.789072172643344	36.0	36.0	36.0	32.0	36.0
36	34.76456584919831	36.0	36.0	36.0	32.0	36.0
37	34.75313746437085	36.0	36.0	36.0	32.0	36.0
38	34.725150097265825	36.0	36.0	36.0	32.0	36.0
39	34.71846371308214	36.0	36.0	36.0	32.0	36.0
40	34.70928340509701	36.0	36.0	36.0	32.0	36.0
41	34.68473043137511	36.0	36.0	36.0	32.0	36.0
42	34.65683123329337	36.0	36.0	36.0	32.0	36.0
43	34.658777949347126	36.0	36.0	36.0	32.0	36.0
44	34.62234128409552	36.0	36.0	36.0	32.0	36.0
45	34.59059763669698	36.0	36.0	36.0	32.0	36.0
46	34.59706523108215	36.0	36.0	36.0	32.0	36.0
47	34.57609733113766	36.0	36.0	36.0	32.0	36.0
48	34.52888258591815	36.0	36.0	36.0	32.0	36.0
49	34.51692378744268	36.0	36.0	36.0	32.0	36.0
50	34.00986746656341	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	16.0
21	56.0
22	152.0
23	506.0
24	1319.0
25	3062.0
26	6590.0
27	12150.0
28	20157.0
29	31004.0
30	45531.0
31	65767.0
32	97278.0
33	161047.0
34	361694.0
35	1337278.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.011768745824206	17.918596708633025	11.271709472851262	26.79792507269151
2	16.811203270139682	20.482506086958956	35.83227938010289	26.874011262798476
3	18.71456308505606	23.785523218439277	28.41512261160908	29.084791084895585
4	12.815542767147726	15.618620568686065	35.49814145123549	36.06769521293072
5	14.747691977551886	36.19422376271803	33.27610899370688	15.781975266023204
6	34.77072702861819	34.914037213997744	16.49569276386195	13.819542993522118
7	30.468835282537405	30.2458469591017	20.642141061107196	18.6431766972537
8	28.417389357205835	32.461221957352315	19.401243696381336	19.720144989060508
9	27.729251123105414	14.014256324611289	18.382774851768747	39.873717700514554
10	16.27268952841235	26.321672319125216	31.153194844211402	26.252443308251035
11	37.87974491628608	20.819785315425847	22.107846110066664	19.19262365822141
12	24.98966696367343	23.44969467393789	28.255139694254083	23.305498668134593
13	29.783122862834187	19.153110873713036	25.05488405073684	26.008882212715932
14	23.69656793913072	19.54632605744515	24.501845018450187	32.255260984973944
15	25.46932511044453	26.845974780860328	21.836294848409928	25.84840526028522
16	26.190445090291607	25.348314292245323	23.471760254897113	24.989480362565953
17	24.491022154216484	25.51919425641791	24.81360881876834	25.176174770597264
18	25.484952953195776	24.229034199317972	25.911336483782033	24.374676363704218
19	26.064862544959205	24.650286199448594	24.909428487458072	24.37542276813413
20	26.045082827566578	24.079660012782174	24.599623998768433	25.27563316088281
21	26.965632741030316	23.936630263900614	23.953704265234816	25.144032729834255
22	26.278474162744153	24.298776363237714	24.29000611118627	25.13274336283186
23	24.889928671726665	23.977775808099423	25.0508254766492	26.081470043524707
24	25.163345944458182	24.80068669207552	24.74913813613484	25.286829227331463
25	25.30469628337244	24.174313424550174	25.01453156124482	25.506458730832566
26	24.799333834046305	24.83987292464581	25.367767457699863	24.993025783608026
27	25.735558240538158	24.556239241279897	24.35060482083961	25.35759769734233
28	24.862918161419287	24.33852239913044	25.282677352690087	25.515882086760183
29	25.015884419274027	24.640722892690366	24.961956699213008	25.38143598882259
30	24.950993884148705	24.649726396126162	25.323636295781416	25.07564342394372
31	25.424214292711827	24.501051963743407	24.390444157286073	25.684289586258696
32	25.187510787876526	24.540051595206215	24.436161428618078	25.83627618829918
33	24.73878177466983	24.179491605282678	25.09332387887722	25.98840274117027
34	25.359930211185805	24.32079529392007	25.3946846674535	24.924589827440627
35	26.00850901050098	24.24004366465915	25.007534019714406	24.743913305125467
36	24.797234571587182	24.57601895867252	24.980896711621984	25.645849758118317
37	25.91296924347246	24.32914569347969	24.46228558366494	25.295599479382908
38	24.96732148105299	24.304094494800825	25.146831746446413	25.58175227769977
39	25.419549265024887	24.17081465378497	24.570187674063845	25.839448407126298
40	25.752492291041744	24.187375502073603	25.25594674404393	24.80418546284072
41	24.746898922845105	24.441013057412494	25.592668442487206	25.21941957725519
42	25.706541768325398	24.745546064815898	25.03836985272508	24.509542314133633
43	24.83357513726844	23.829614528762228	25.36800070908421	25.968809624885125
44	24.51985202532177	24.357975564584976	25.219932730300755	25.902239679792498
45	24.59523887274271	24.529415332079996	25.33567206721372	25.539673727963574
46	24.52717523649402	24.173378876528613	25.472311609502867	25.8271342774745
47	24.87510548798778	23.896009020301744	25.749658636439758	25.479226855270714
48	25.15896081843246	24.868376243813007	24.90373715368001	25.06892578407453
49	24.359386436612272	24.580975355113736	25.309886271236966	25.749751937037026
50	23.99468559550067	25.200922929938685	25.162856268496853	25.641535206063796
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12.0
1	20.5
2	29.0
3	81.5
4	134.0
5	183.0
6	232.0
7	238.0
8	244.0
9	345.5
10	447.0
11	644.5
12	842.0
13	1435.0
14	2028.0
15	2813.5
16	3599.0
17	4244.5
18	4890.0
19	5372.0
20	5854.0
21	6865.0
22	7876.0
23	8400.5
24	8925.0
25	11291.0
26	13657.0
27	17260.5
28	20864.0
29	24049.5
30	27235.0
31	29805.0
32	32375.0
33	37974.5
34	43574.0
35	51359.5
36	59145.0
37	70232.0
38	81319.0
39	86431.5
40	91544.0
41	99623.0
42	107702.0
43	109347.5
44	110993.0
45	121737.0
46	132481.0
47	141076.5
48	149672.0
49	154517.0
50	159362.0
51	154379.5
52	149397.0
53	148378.0
54	147359.0
55	149452.0
56	151545.0
57	149517.5
58	147490.0
59	135186.5
60	122883.0
61	109187.0
62	95491.0
63	83996.0
64	72501.0
65	62371.0
66	52241.0
67	46445.0
68	40649.0
69	37215.5
70	33782.0
71	27056.0
72	20330.0
73	18399.0
74	16468.0
75	12431.5
76	8395.0
77	7634.5
78	6874.0
79	5873.5
80	4873.0
81	3968.5
82	3064.0
83	2689.0
84	2314.0
85	1980.5
86	1647.0
87	1246.0
88	845.0
89	573.5
90	302.0
91	189.0
92	76.0
93	50.5
94	25.0
95	18.0
96	11.0
97	11.0
98	11.0
99	6.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014274984722034326
2	0.004338475748853569
3	5.598033224327186E-4
4	9.330055373878644E-5
5	0.0
6	3.7320221495514575E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.8660110747757287E-4
47	4.665027686939322E-5
48	0.0
49	4.665027686939322E-5
50	9.330055373878644E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2143610.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.92658622021036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.81354552958679	44.388121762917436
2	11.761822654397292	12.920735322671526
3	3.1596878570006406	5.206526025194922
4	1.3106686941269583	2.8796222813637957
5	0.7314890394926881	2.0089097898416997
6	0.4170045125207815	1.3742780586713699
7	0.30609895720156716	1.176907953525385
8	0.21730574679798792	0.9548690270117508
9	0.1727554072075047	0.85399782920915
>10	0.9309619951811575	9.340106392866533
>50	0.0872915707216307	3.3891020257879245
>100	0.07787928976860099	9.169769072919125
>500	0.010500732642862545	3.928924996478326
>1k	0.00298801335366007	2.408129461541101
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2225	0.10379686603439991	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2169	0.1011844505297139	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.665027686939322E-5
2	0.0	0.0	0.0	0.0	4.665027686939322E-5
3	0.0	0.0	0.0	0.0	4.665027686939322E-5
4	0.0	0.0	0.0	0.0	4.665027686939322E-5
5	0.0	0.0	0.0	0.0	4.665027686939322E-5
6	0.0	0.0	0.0	0.0	9.330055373878644E-5
7	0.0	0.0	0.0	0.0	9.330055373878644E-5
8	0.0	0.0	0.0	0.0	9.330055373878644E-5
9	0.0	0.0	0.0	0.0	9.330055373878644E-5
10	4.665027686939322E-5	0.0	0.0	0.0	9.330055373878644E-5
11	4.665027686939322E-5	0.0	0.0	0.0	9.330055373878644E-5
12	4.665027686939322E-5	0.0	0.0	0.0	2.332513843469661E-4
13	4.665027686939322E-5	0.0	0.0	0.0	4.1985249182453896E-4
14	4.665027686939322E-5	0.0	0.0	0.0	4.1985249182453896E-4
15	4.665027686939322E-5	0.0	0.0	4.665027686939322E-5	4.665027686939322E-4
16	4.665027686939322E-5	0.0	0.0	9.330055373878644E-5	5.598033224327186E-4
17	4.665027686939322E-5	0.0	0.0	9.330055373878644E-5	5.598033224327186E-4
18	4.665027686939322E-5	0.0	0.0	1.3995083060817966E-4	6.064535993021118E-4
19	4.665027686939322E-5	0.0	0.0	1.3995083060817966E-4	6.064535993021118E-4
20	4.665027686939322E-5	0.0	0.0	3.2655193808575254E-4	6.997541530408983E-4
21	4.665027686939322E-5	0.0	0.0	3.2655193808575254E-4	7.464044299102915E-4
22	4.665027686939322E-5	0.0	0.0	4.1985249182453896E-4	7.464044299102915E-4
23	4.665027686939322E-5	0.0	0.0	9.330055373878644E-4	7.464044299102915E-4
24	4.665027686939322E-5	0.0	0.0	0.0016327596904287627	7.464044299102915E-4
25	4.665027686939322E-5	0.0	0.0	0.001866011074775729	7.464044299102915E-4
26	4.665027686939322E-5	0.0	0.0	0.002938967442771773	8.397049836490779E-4
27	4.665027686939322E-5	0.0	0.0	0.0049915796250250746	8.863552605184712E-4
28	4.665027686939322E-5	0.0	0.0	0.013761831676471	8.863552605184712E-4
29	4.665027686939322E-5	0.0	0.0	0.02952962525832591	8.863552605184712E-4
30	4.665027686939322E-5	0.0	0.0	0.06013220688464786	8.863552605184712E-4
31	4.665027686939322E-5	0.0	0.0	0.13449274821446064	9.796558142572576E-4
32	4.665027686939322E-5	0.0	0.0	0.2168771371658091	9.796558142572576E-4
33	4.665027686939322E-5	0.0	0.0	0.30042778303889234	9.796558142572576E-4
34	4.665027686939322E-5	0.0	0.0	0.3949878942531524	0.0011662569217348305
35	4.665027686939322E-5	0.0	0.0	0.5015371266228466	0.0011662569217348305
36	4.665027686939322E-5	0.0	0.0	0.6530572258946357	0.0011662569217348305
37	4.665027686939322E-5	0.0	0.0	0.875532396284772	0.0011662569217348305
38	4.665027686939322E-5	0.0	0.0	1.1651839653668346	0.0011662569217348305
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTATA	65	4.934034E-4	20.307594	9
TAGCGGC	810	0.0	17.92584	30
TAGGACG	655	0.0	17.801443	4
CGCAATA	795	0.0	17.710608	36
ATACGAA	770	0.0	17.428488	40
TGTAGGA	815	0.0	17.276798	2
TATACCG	90	2.216458E-4	17.11103	5
GTATCAA	3645	0.0	16.962908	1
CTAGCGG	820	0.0	16.902357	29
CAATACG	825	0.0	16.79992	38
CGTCGTA	695	0.0	16.143808	10
TCTAGCG	845	0.0	16.141933	28
CCTAATA	150	1.8386527E-7	16.134008	2
CGAATGC	830	0.0	15.903538	43
AATACGA	860	0.0	15.86039	39
GTCCTAA	500	0.0	15.842511	1
ATACCGT	750	0.0	15.839926	6
GCATTCG	865	0.0	15.768711	22
ACCGTCG	740	0.0	15.756681	8
TAATACT	545	0.0	15.743043	4
>>END_MODULE