FastQCFastQC Report
Thu 2 Feb 2017
SRR4062570_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062570_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345575
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51290.21866706457904778No Hit
TATCAACGCAGAGTACTTTTTTTTT31720.13523336495315647No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30190.12871044413416752No Hit
GTACATGGAAGCAGTGGTATCAACG24370.10389776494036643No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGTA500.001505897113.293012
GACCCGT655.4532953E-513.1542316
CGAACGA1700.012.85578716
CGTCGTA2750.012.783010510
TACCGTC3150.012.365447
ACGAACG1850.012.32652815
GTATTAT1353.765308E-1011.954891
ACGCTTA802.8600756E-511.87813717
CGCTTAC1052.7118767E-711.76526218
ATACCGT3500.011.4003336
CCGTCGT3100.011.3397679
GTCCTAC4950.011.3155921
ACCGTCG3050.011.2136828
TCGACGC855.3179298E-511.17799114
TAGCGCC600.00588897211.0803464
TAGAACC2500.011.017034
AACCGCG1658.0035534E-1110.93971357
CCGACCA2450.010.8581569
GTCTTAG2200.010.788171
ATATACG2650.010.7550316
Produced by FastQC (version 0.11.5)