##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062570_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2345575
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.178242435223773	32.0	32.0	32.0	32.0	32.0
2	30.833613079948414	32.0	32.0	32.0	32.0	32.0
3	30.822598296792897	32.0	32.0	32.0	32.0	32.0
4	30.895255534357247	32.0	32.0	32.0	32.0	32.0
5	30.683294288180935	32.0	32.0	32.0	32.0	32.0
6	34.34030418980421	36.0	36.0	36.0	32.0	36.0
7	34.2246408663121	36.0	36.0	36.0	32.0	36.0
8	34.22599192095755	36.0	36.0	36.0	32.0	36.0
9	34.389989661383666	36.0	36.0	36.0	32.0	36.0
10	34.060494335077756	36.0	36.0	36.0	32.0	36.0
11	34.37771889621948	36.0	36.0	36.0	32.0	36.0
12	34.160971616767746	36.0	36.0	36.0	32.0	36.0
13	34.279519947134496	36.0	36.0	36.0	32.0	36.0
14	34.18328469565032	36.0	36.0	36.0	32.0	36.0
15	34.12158170171493	36.0	36.0	36.0	32.0	36.0
16	34.126188248084155	36.0	36.0	36.0	32.0	36.0
17	34.05058972746555	36.0	36.0	36.0	32.0	36.0
18	34.06345736120141	36.0	36.0	36.0	32.0	36.0
19	34.056079212986155	36.0	36.0	36.0	32.0	36.0
20	34.03567611353293	36.0	36.0	36.0	32.0	36.0
21	34.028243820811525	36.0	36.0	36.0	32.0	36.0
22	33.97495923174488	36.0	36.0	36.0	32.0	36.0
23	33.93580422710849	36.0	36.0	36.0	32.0	36.0
24	33.91505792822655	36.0	36.0	36.0	32.0	36.0
25	33.4539113010669	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	13.0
4	81.0
5	240.0
6	385.0
7	108.0
8	337.0
9	425.0
10	254.0
11	85.0
12	161.0
13	151.0
14	411.0
15	523.0
16	786.0
17	1159.0
18	1457.0
19	1939.0
20	2997.0
21	4691.0
22	6844.0
23	10117.0
24	13983.0
25	19813.0
26	27471.0
27	35614.0
28	48469.0
29	63993.0
30	82560.0
31	113465.0
32	162584.0
33	236031.0
34	526797.0
35	981631.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.62455897244577	17.28218301977296	10.944859564035601	26.14839844374567
2	16.928124220583626	19.866979605671272	36.84187413797881	26.36302203576629
3	19.302339363546793	23.703808239467875	28.095939955667674	28.897912441317658
4	12.883577105148927	15.08490013329206	35.568299174578485	36.46322358698053
5	14.763824946209962	36.30807351696225	33.52741106251586	15.400690474311924
6	34.49442055399493	34.877061102206106	16.8311937139635	13.797324629835469
7	30.32933475199211	30.353908151798763	20.76209109393438	18.554666002274747
8	28.17039517955278	32.752013320150866	19.581253304328495	19.496338195967855
9	27.382033955444413	14.430701149842214	18.63290609870848	39.55435879600489
10	15.85359034782835	26.738874986074656	31.7943394607681	25.613195205328893
11	37.18425189590786	21.367512247050442	22.103850548636274	19.344385308405425
12	24.57999748984626	23.762148418389696	28.80764282049071	22.85021127127334
13	29.374339982806475	19.674765723242253	25.278882608498932	25.672011685452347
14	23.353964886519343	19.602166739944252	25.204657893276533	31.839210480259872
15	25.112307253010474	27.134926200529858	22.432256051573624	25.320510494886044
16	25.689441916327144	25.890723872398507	23.767062657758363	24.65277155351599
17	24.026429153170938	25.84922799007647	25.324665900604256	24.799676956148335
18	24.721253306775257	25.071685039181844	26.236446863851576	23.970614790191327
19	25.278066759928336	25.35046763881353	25.46005080868281	23.911414792575325
20	25.343792191028815	24.635142974098574	25.345072728103133	24.675992106769474
21	26.132720103441994	24.467240879564272	24.7963240357061	24.603714981287638
22	25.457757062976714	24.714526282710352	25.027881294853444	24.799835359459486
23	24.391396620997664	24.53732225186559	25.533185699971266	25.53809542716548
24	24.477434806088535	25.260295376392516	25.405656933917058	24.85661288360189
25	24.671049682671747	24.894867807286747	25.440688623866446	24.99339388617506
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	283.0
1	283.0
2	789.0
3	1295.0
4	1295.0
5	1295.0
6	3412.0
7	5529.0
8	5529.0
9	5529.0
10	5857.0
11	6185.0
12	6185.0
13	6185.0
14	7007.5
15	7830.0
16	7830.0
17	7830.0
18	12534.5
19	17239.0
20	17239.0
21	17239.0
22	27016.0
23	36793.0
24	36793.0
25	36793.0
26	53251.5
27	69710.0
28	69710.0
29	69710.0
30	86347.5
31	102985.0
32	102985.0
33	102985.0
34	124972.0
35	146959.0
36	146959.0
37	146959.0
38	167311.0
39	187663.0
40	187663.0
41	187663.0
42	213701.5
43	239740.0
44	239740.0
45	239740.0
46	272814.0
47	305888.0
48	305888.0
49	305888.0
50	307748.0
51	309608.0
52	309608.0
53	309608.0
54	290433.0
55	271258.0
56	271258.0
57	271258.0
58	247507.5
59	223757.0
60	223757.0
61	223757.0
62	197879.0
63	172001.0
64	172001.0
65	172001.0
66	142476.0
67	112951.0
68	112951.0
69	112951.0
70	86445.5
71	59940.0
72	59940.0
73	59940.0
74	46649.0
75	33358.0
76	33358.0
77	33358.0
78	26437.5
79	19517.0
80	19517.0
81	19517.0
82	13974.0
83	8431.0
84	8431.0
85	8431.0
86	6176.0
87	3921.0
88	3921.0
89	3921.0
90	2639.0
91	1357.0
92	1357.0
93	1357.0
94	829.0
95	301.0
96	301.0
97	301.0
98	688.5
99	1076.0
100	1076.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008526693881031304
2	0.002984342858360956
3	0.004604414695756904
4	0.014879080822399623
5	0.03453311021817678
6	0.04591624654935357
7	0.06723298125193182
8	0.08773968003581212
9	0.09421996738539591
10	0.11613357065964636
11	0.11792417637466292
12	0.13169478699252848
13	0.12078061882480841
14	0.12299755923387656
15	0.11391663025057822
16	0.11054858616757085
17	0.12188908902934249
18	0.1294352131140552
19	0.13054368331858926
20	0.11971478208967949
21	0.12815620903190048
22	0.1493450433262633
23	0.14009358046534431
24	0.13386909393219149
25	0.12994681474691705
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2345575.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.5511789753808
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.25853923081874	42.69095579932253
2	13.29764830694144	14.508047854872641
3	3.825945941108992	6.261295853507051
4	1.5519965344957491	3.386529628897935
5	0.799227885710189	2.1799411717745856
6	0.5018524952180023	1.6425987171527208
7	0.31193357598015564	1.191144103220681
8	0.21853239250360323	0.9536959724305781
9	0.16437602975295793	0.8070215596484515
>10	0.9044903316465994	8.916840540202152
>50	0.0779980862270703	2.953380705797387
>100	0.0752960671429578	8.619466479129732
>500	0.009259667416177378	3.326766912128952
>1k	0.002824983279511583	2.342756219095222
>5k	7.847175776421062E-5	0.21955848281938664
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5129	0.21866706457904778	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3172	0.13523336495315647	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3019	0.12871044413416752	No Hit
GTACATGGAAGCAGTGGTATCAACG	2437	0.10389776494036643	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.2790040821546956E-4	0.0
8	0.0	0.0	0.0	1.7053387762062608E-4	0.0
9	0.0	0.0	0.0	1.7053387762062608E-4	0.0
10	0.0	0.0	0.0	1.7053387762062608E-4	4.263346940515652E-5
11	0.0	0.0	0.0	1.7053387762062608E-4	4.263346940515652E-5
12	0.0	0.0	0.0	2.558008164309391E-4	1.2790040821546956E-4
13	0.0	4.263346940515652E-5	0.0	2.558008164309391E-4	1.7053387762062608E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTAGTA	50	0.0015058971	13.29301	2
GACCCGT	65	5.4532953E-5	13.154231	6
CGAACGA	170	0.0	12.855787	16
CGTCGTA	275	0.0	12.7830105	10
TACCGTC	315	0.0	12.36544	7
ACGAACG	185	0.0	12.326528	15
GTATTAT	135	3.765308E-10	11.95489	1
ACGCTTA	80	2.8600756E-5	11.878137	17
CGCTTAC	105	2.7118767E-7	11.765262	18
ATACCGT	350	0.0	11.400333	6
CCGTCGT	310	0.0	11.339767	9
GTCCTAC	495	0.0	11.315592	1
ACCGTCG	305	0.0	11.213682	8
TCGACGC	85	5.3179298E-5	11.177991	14
TAGCGCC	60	0.005888972	11.080346	4
TAGAACC	250	0.0	11.01703	4
AACCGCG	165	8.0035534E-11	10.9397135	7
CCGACCA	245	0.0	10.858156	9
GTCTTAG	220	0.0	10.78817	1
ATATACG	265	0.0	10.755031	6
>>END_MODULE