FastQCFastQC Report
Thu 2 Feb 2017
SRR4062570_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062570_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345575
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT250.002353315235.19977610
TAGAACG1101.2669989E-719.9998744
TAGGACG3650.017.4793424
GTATCAA49600.017.1248761
CGCAATA5900.016.40667536
ATACCGT4250.016.0469596
GTCCTAC6100.015.1509991
TACCGTC4650.015.139697
GTATAGA3100.014.9066281
AACGCAG57100.014.7950046
TATACTC2551.2732926E-1114.6665755
GGCGTTA4650.014.666574542
TATACGG1057.162719E-414.66657455
TCCTACA8300.014.3137732
GTCTTAG3850.014.2889751
CTAATAG1851.2672535E-714.270183
GTACTAA1707.5795833E-714.2385431
ATACGAA6500.014.21529640
GATATAC2204.8894435E-914.0031951
ACCGGTA950.006242403813.894649512