##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062570_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2345575
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.256869637508927	32.0	32.0	32.0	32.0	32.0
2	31.399722882448867	32.0	32.0	32.0	32.0	32.0
3	31.490877929718724	32.0	32.0	32.0	32.0	32.0
4	31.595514959018576	32.0	32.0	32.0	32.0	32.0
5	31.523458427038147	32.0	32.0	32.0	32.0	32.0
6	35.12015433315925	36.0	36.0	36.0	36.0	36.0
7	35.13473412702642	36.0	36.0	36.0	36.0	36.0
8	35.07813009603189	36.0	36.0	36.0	36.0	36.0
9	35.204204086418045	36.0	36.0	36.0	36.0	36.0
10	35.0832678554299	36.0	36.0	36.0	36.0	36.0
11	35.21445573047121	36.0	36.0	36.0	36.0	36.0
12	35.130469501081826	36.0	36.0	36.0	36.0	36.0
13	35.17029001417563	36.0	36.0	36.0	36.0	36.0
14	35.135799537426855	36.0	36.0	36.0	36.0	36.0
15	35.098942050456714	36.0	36.0	36.0	36.0	36.0
16	35.10684245867218	36.0	36.0	36.0	36.0	36.0
17	35.08653954787206	36.0	36.0	36.0	36.0	36.0
18	35.09584046555749	36.0	36.0	36.0	36.0	36.0
19	35.07656971105166	36.0	36.0	36.0	36.0	36.0
20	35.07206292700084	36.0	36.0	36.0	36.0	36.0
21	35.06588533728404	36.0	36.0	36.0	36.0	36.0
22	35.05139294202914	36.0	36.0	36.0	36.0	36.0
23	35.012789614486856	36.0	36.0	36.0	36.0	36.0
24	34.99146605842917	36.0	36.0	36.0	36.0	36.0
25	34.96934866717116	36.0	36.0	36.0	36.0	36.0
26	34.92611577118617	36.0	36.0	36.0	32.0	36.0
27	34.90711190219882	36.0	36.0	36.0	32.0	36.0
28	34.894605800283514	36.0	36.0	36.0	32.0	36.0
29	34.86832525073809	36.0	36.0	36.0	32.0	36.0
30	34.861475331208766	36.0	36.0	36.0	32.0	36.0
31	34.85568613239824	36.0	36.0	36.0	32.0	36.0
32	34.816197307696406	36.0	36.0	36.0	32.0	36.0
33	34.79693507988446	36.0	36.0	36.0	32.0	36.0
34	34.784961470002024	36.0	36.0	36.0	32.0	36.0
35	34.755772465173784	36.0	36.0	36.0	32.0	36.0
36	34.72886435095872	36.0	36.0	36.0	32.0	36.0
37	34.71822857934622	36.0	36.0	36.0	32.0	36.0
38	34.68807776344819	36.0	36.0	36.0	32.0	36.0
39	34.680836895004425	36.0	36.0	36.0	32.0	36.0
40	34.6693352376283	36.0	36.0	36.0	32.0	36.0
41	34.63570126727988	36.0	36.0	36.0	32.0	36.0
42	34.61133538684544	36.0	36.0	36.0	32.0	36.0
43	34.597517879411235	36.0	36.0	36.0	32.0	36.0
44	34.56816601472986	36.0	36.0	36.0	32.0	36.0
45	34.526541679545524	36.0	36.0	36.0	32.0	36.0
46	34.52316638777272	36.0	36.0	36.0	32.0	36.0
47	34.49348454003816	36.0	36.0	36.0	32.0	36.0
48	34.453544226895325	36.0	36.0	36.0	32.0	36.0
49	34.4388672287179	36.0	36.0	36.0	32.0	36.0
50	33.91416347803843	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	15.0
21	52.0
22	209.0
23	571.0
24	1568.0
25	3658.0
26	7714.0
27	13870.0
28	23313.0
29	35136.0
30	50994.0
31	73576.0
32	109659.0
33	180460.0
34	402960.0
35	1441816.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.02808626801177	17.882503135123056	11.455085124827146	26.63432547203803
2	16.403387785468677	20.291410944857887	36.48784792910635	26.81735334056709
3	18.71004957018848	23.88253907484045	28.430999295265146	28.97641205970592
4	12.588858846491307	15.547026015806791	35.70669695349624	36.15741818420566
5	14.509363375718106	36.52865502062394	33.50653890837002	15.455442695287935
6	34.766066824894494	35.053972116715684	16.51245351329297	13.66750754509685
7	30.400034447872653	30.363668067461553	20.7313095776597	18.504987907006093
8	28.240452767445085	32.88059431056351	19.405902603839145	19.473050318152264
9	27.420142182620467	14.228280911929911	18.55792289737058	39.793654008079045
10	15.771058269294311	26.757490167656115	31.915202029353146	25.55624953369643
11	37.433593042217794	21.177621691909234	22.144165076793538	19.244620189079438
12	24.561610692474126	23.65927331251399	28.74310137173188	23.036014623280007
13	29.499248585101732	19.534485147565096	25.29490636624282	25.67135990109035
14	23.38032252219605	19.460473444677746	25.241827696833397	31.917376336292808
15	25.299723948285603	26.985110260810245	22.403973439348558	25.31119235155559
16	25.818956972171	25.760932820310583	23.714824723148908	24.705285484369504
17	24.162518785372455	25.794485360732445	25.335663962994147	24.707331890900953
18	24.874796158724408	24.92246037751937	26.17669441394967	24.02604904980655
19	25.396374023427093	25.195783549875827	25.493493066732036	23.91434935996504
20	25.475118041418416	24.48103343529838	25.31980431237543	24.724044210907774
21	26.29781610052972	24.328618782174946	24.719525063150826	24.654040054144506
22	25.434104643850652	24.67292668109099	25.0739797277853	24.818988947273056
23	24.46615435447598	24.423265084254396	25.526448738582225	25.5841318226874
24	24.58957824840391	25.14534815556953	25.326455133602632	24.938618462423925
25	24.832205322788656	24.593244726772753	25.457595685492894	25.116954264945697
26	24.3844686270957	25.161165172718842	25.803523656246334	24.65084254393912
27	25.136736194749687	25.030792023277876	25.051042921245326	24.781428860727114
28	24.41205248180084	24.79068032358803	25.773339159907486	25.023928034703648
29	24.54911908593842	25.057906909819554	25.586902998198735	24.806071006043297
30	24.610553915351247	25.0657514681901	25.794655894610063	24.529038721848586
31	24.814896134210162	24.885283992198076	25.104718459226415	25.195101414365347
32	24.544983639406116	25.040128753077607	25.161975208637543	25.252912398878742
33	24.407362800166272	24.728605992134124	25.522824893682788	25.341206314016816
34	24.84763863871332	24.780959892563654	25.84214957952741	24.529251889195614
35	25.41372584547499	24.751585432143504	25.411167837310682	24.423520885070822
36	24.450038903040834	25.09938927555077	25.370580774436974	25.079991046971422
37	25.297592274815344	24.877567334235742	25.02175372776398	24.803086663184935
38	24.497660488366392	24.806326806859722	25.572748686356224	25.123264018417657
39	24.85509949585922	24.836895004423223	25.058162710635983	25.249842789081566
40	25.090990482077956	24.832716924421515	25.600630975347194	24.47566161815333
41	24.3538577960628	25.013866535924027	25.8206196774778	24.81165599053537
42	25.14956886904064	25.173187811091097	25.372669814437828	24.304573505430437
43	24.472890442641994	24.456007588757554	25.71450497212837	25.35659699647208
44	24.280570862155333	24.843545825650427	25.531266320625008	25.34461699156923
45	24.33245579442141	25.053515662470822	25.64249704230306	24.971531500804705
46	24.287402571822504	24.678223752480204	25.70709770827951	25.327275967417783
47	24.483473981208864	24.62100961214611	25.889782202565343	25.005734204079683
48	24.74186500111913	25.350329876469523	25.216034447843278	24.69177067456807
49	24.04084286369014	25.11691163147629	25.64637668801893	25.19586881681464
50	23.869040158187293	25.49844942005667	25.427677830671726	25.204832591084315
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	44.5
2	59.0
3	197.0
4	335.0
5	383.0
6	431.0
7	382.0
8	333.0
9	491.0
10	649.0
11	939.0
12	1229.0
13	2152.0
14	3075.0
15	4182.0
16	5289.0
17	6192.0
18	7095.0
19	7708.5
20	8322.0
21	9811.5
22	11301.0
23	12163.5
24	13026.0
25	15431.5
26	17837.0
27	21899.5
28	25962.0
29	29741.0
30	33520.0
31	36428.5
32	39337.0
33	45361.5
34	51386.0
35	58954.5
36	66523.0
37	77866.0
38	89209.0
39	95926.0
40	102643.0
41	112159.0
42	121675.0
43	125300.5
44	128926.0
45	140448.5
46	151971.0
47	160472.5
48	168974.0
49	172502.5
50	176031.0
51	171757.5
52	167484.0
53	164269.5
54	161055.0
55	160578.0
56	160101.0
57	155982.5
58	151864.0
59	139153.0
60	126442.0
61	111680.5
62	96919.0
63	85403.0
64	73887.0
65	63073.5
66	52260.0
67	46183.5
68	40107.0
69	36021.0
70	31935.0
71	25506.5
72	19078.0
73	16869.0
74	14660.0
75	11256.0
76	7852.0
77	6889.0
78	5926.0
79	5201.0
80	4476.0
81	3487.0
82	2498.0
83	2179.0
84	1860.0
85	1515.5
86	1171.0
87	857.5
88	544.0
89	366.5
90	189.0
91	125.5
92	62.0
93	41.0
94	20.0
95	14.5
96	9.0
97	6.5
98	4.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015859650618718225
2	0.0038370122464640864
3	5.116016328618782E-4
4	4.263346940515652E-5
5	0.0
6	5.116016328618782E-4
7	8.526693881031304E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	4.263346940515652E-5
47	4.263346940515652E-5
48	0.0
49	0.0
50	4.263346940515652E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2345575.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.42249117767787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.7854031211105	45.24054115651763
2	13.220373912512533	15.182936087137074
3	3.6986831374625626	6.371627994899098
4	1.4518394392346632	3.3347294956342894
5	0.7444402421459758	2.137380661846784
6	0.46586857848960794	1.6050800602966109
7	0.3095092051118535	1.244095271996185
8	0.2128765576084862	0.9779121800966187
9	0.15531516602260947	0.8026725375623579
>10	0.8130920103431787	8.433417878489681
>50	0.07085668145569281	2.837566926162828
>100	0.06262043104945306	7.628273871231331
>500	0.007177587503811134	2.7355926489574682
>1k	0.0019439299489485838	1.4681732291721095
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2790040821546956E-4
2	0.0	0.0	0.0	0.0	1.2790040821546956E-4
3	0.0	0.0	0.0	0.0	1.2790040821546956E-4
4	0.0	0.0	0.0	0.0	1.2790040821546956E-4
5	0.0	0.0	0.0	0.0	1.2790040821546956E-4
6	0.0	0.0	0.0	0.0	2.131673470257826E-4
7	0.0	0.0	0.0	0.0	2.131673470257826E-4
8	0.0	0.0	0.0	0.0	2.131673470257826E-4
9	0.0	0.0	0.0	0.0	2.131673470257826E-4
10	0.0	0.0	0.0	0.0	2.131673470257826E-4
11	0.0	0.0	0.0	0.0	2.131673470257826E-4
12	0.0	0.0	0.0	4.263346940515652E-5	2.558008164309391E-4
13	0.0	0.0	0.0	4.263346940515652E-5	3.4106775524125215E-4
14	0.0	0.0	0.0	4.263346940515652E-5	3.4106775524125215E-4
15	0.0	0.0	0.0	8.526693881031304E-5	4.689681634567217E-4
16	0.0	0.0	0.0	2.558008164309391E-4	4.689681634567217E-4
17	0.0	0.0	0.0	2.558008164309391E-4	5.116016328618782E-4
18	0.0	0.0	0.0	4.689681634567217E-4	5.542351022670347E-4
19	0.0	0.0	0.0	5.116016328618782E-4	5.542351022670347E-4
20	0.0	4.263346940515652E-5	0.0	7.247689798876608E-4	6.395020410773478E-4
21	0.0	4.263346940515652E-5	0.0	9.379363269134434E-4	6.395020410773478E-4
22	0.0	4.263346940515652E-5	0.0	0.001406904490370165	7.674024492928173E-4
23	0.0	4.263346940515652E-5	0.0	0.002387474286688765	8.100359186979739E-4
24	0.0	4.263346940515652E-5	0.0	0.0035812114300331475	8.100359186979739E-4
25	0.0	4.263346940515652E-5	0.0	0.004476514287541434	8.100359186979739E-4
26	0.0	4.263346940515652E-5	0.0	0.005840785308506443	8.100359186979739E-4
27	0.0	4.263346940515652E-5	0.0	0.007801924901143643	8.100359186979739E-4
28	0.0	4.263346940515652E-5	0.0	0.015475949394071816	8.100359186979739E-4
29	0.0	4.263346940515652E-5	0.0	0.028223356746213617	8.100359186979739E-4
30	0.0	4.263346940515652E-5	0.0	0.053163936348230176	8.100359186979739E-4
31	0.0	4.263346940515652E-5	0.0	0.10760687677861505	8.100359186979739E-4
32	0.0	4.263346940515652E-5	0.0	0.17394455517303858	8.100359186979739E-4
33	0.0	4.263346940515652E-5	0.0	0.23913112989352292	8.526693881031304E-4
34	0.0	4.263346940515652E-5	0.0	0.3159140082922098	8.953028575082869E-4
35	0.0	4.263346940515652E-5	0.0	0.40292891934813424	8.953028575082869E-4
36	0.0	4.263346940515652E-5	0.0	0.5224731675601931	8.953028575082869E-4
37	0.0	4.263346940515652E-5	0.0	0.6989757308975412	8.953028575082869E-4
38	0.0	4.263346940515652E-5	0.0	0.9293243660936018	8.953028575082869E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACGT	25	0.0023533152	35.199776	10
TAGAACG	110	1.2669989E-7	19.999874	4
TAGGACG	365	0.0	17.479342	4
GTATCAA	4960	0.0	17.124876	1
CGCAATA	590	0.0	16.406675	36
ATACCGT	425	0.0	16.046959	6
GTCCTAC	610	0.0	15.150999	1
TACCGTC	465	0.0	15.13969	7
GTATAGA	310	0.0	14.906628	1
AACGCAG	5710	0.0	14.795004	6
TATACTC	255	1.2732926E-11	14.666575	5
GGCGTTA	465	0.0	14.6665745	42
TATACGG	105	7.162719E-4	14.6665745	5
TCCTACA	830	0.0	14.313773	2
GTCTTAG	385	0.0	14.288975	1
CTAATAG	185	1.2672535E-7	14.27018	3
GTACTAA	170	7.5795833E-7	14.238543	1
ATACGAA	650	0.0	14.215296	40
GATATAC	220	4.8894435E-9	14.003195	1
ACCGGTA	95	0.0062424038	13.8946495	12
>>END_MODULE