FastQCFastQC Report
Thu 2 Feb 2017
SRR4062569_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062569_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1430985
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT162951.1387261222165153No Hit
GTCCTACAGTGGACATTTCTAAATT161241.1267763114218527No Hit
CTGTAGGACGTGGAATATGGCAAGA153021.0693333612861071No Hit
CTTTAGGACGTGAAATATGGCGAGG132910.9288007910634981No Hit
GTCCTACAGTGTGCATTTCTCATTT99870.6979108795689682No Hit
CTGTAGGACCTGGAATATGGCGAGA80780.5645062666624737No Hit
CTGAAGGACCTGGAATATGGCGAGA72230.5047572126891616No Hit
GTCCTTCAGTGTGCATTTCTCATTT66310.4633871074819093No Hit
GTACATGGAAGCAGTGGTATCAACG60000.4192916068302603No Hit
ATTTAGAAATGTCCACTGTAGGACG59860.4183132597476563No Hit
TCCATGTACTCTGCGTTGATACCAC50440.3524844774753055No Hit
TTTCTAAATTTTCCACCTTTTTCAG47540.33221871647850953No Hit
GAATATGGCAAGAAAACTGAAAATC41270.2884027435647474No Hit
GGAATATGGCGAGAAAACTGAAAAT40720.28455923716880327No Hit
ATTCCAGGTCCTTCAGTGTGCATTT35010.24465665258545685No Hit
GATATACACTGTTCTACAAATCCCG34170.23878657008983323No Hit
CTGTAGGACATGGAATATGGCAAGA33110.231379085035832No Hit
TTGTAGAACAGTGTATATCAATGAG32730.22872357152590697No Hit
ACCTGGAATATGGCGAGAAAACTGA32350.226068058015982No Hit
GACCTGGAATATGGCGAGAAAACTG30410.21251096272847025No Hit
CCATATTCCAGGTCCTTCAGTGTGC29760.20796863698780912No Hit
GCCATATTCCACGTCCTACAGTGGA29490.20608182475707293No Hit
GTATCAACGCAGAGTACTTTTTTTT29350.20510347767446901No Hit
TTCCAGGTCCTTCAGTGTGCATTTC28760.2009804435406381No Hit
GTCCACTGTAGGACGTGGAATATGG28590.19979245065461904No Hit
ACAGTGGACATTTCTAAATTTTCCA28380.1983249300307131No Hit
GAGTACATGGAAGCAGTGGTATCAA27420.19161626432142895No Hit
GAAATATGGCGAGGAAAACTGAAAA27340.19105720884565525No Hit
CCATATTTCACGTCCTAAAGTGTGT27070.1891703966149191No Hit
GTGTATATCAATGAGTTACAATGAA26490.1851172444155599No Hit
ATACACACTTTAGGACGTGAAATAT26320.18392925152954084No Hit
GTGTATATCAATGAGTTACAATGAG25600.17889775224757773No Hit
CACTTTAGGACGTGAAATATGGCGA25250.17645188454106786No Hit
CCACTGTAGGACGTGGAATATGGCA24680.1724686142761804No Hit
GTTCTACAGTGTGGTTTTTATCATT24430.17072156591438764No Hit
CCTAAAGTGTGTATTTCTCATTTTC24270.1696034549628403No Hit
ATTCCACGTCCTACAGTGGACATTT24080.1682756982078778No Hit
GAAATACACACTTTAGGACGTGAAA23780.16617924017372648No Hit
GTGTATTTCTCATTTTCCGTGATTT23400.16352372666380152No Hit
CATGTACTCTGCGTTGATACCACTG23350.16317431699144294No Hit
AGTGTGTATTTCTCATTTTCCGTGA21750.15199320747596937No Hit
CTACAGTGGACATTTCTAAATTTTC21230.14835934688344043No Hit
TATCAACGCAGAGTACTTTTTTTTT20520.14339772953594904No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20420.1426989101912319No Hit
GTCCTACAGTGGACATTTCTAAATA20260.14158079923968456No Hit
GTAGGACGTGGAATATGGCAAGAAA19740.13794693864715563No Hit
GGTCCTTCAGTGTGCATTTCTCATT19500.1362697722198346No Hit
GATATACACTGTTCTACAATGCCGG19070.13326484903755106No Hit
TCCTAAAGTGTGTATTTCTCATTTT18920.1322166200204754No Hit
GTTCTACAAATCCCGTTTCCAACGA17470.12208373952207745No Hit
ACGTGGAATATGGCAAGAAAACTGA17160.11991739955345443No Hit
CCTTCAGTGTGCATTTCTCATTTTT16440.1148859002714913No Hit
CCCATGTACTCTGCGTTGATACCAC16380.11446660866466106No Hit
ATATTTCACGTCCTAAAGTGTGTAT16330.11411719899230251No Hit
TTTCTAAATATTCCACCTTTTTCAG16280.11376778931994394No Hit
ACGTCCTACAGTGGACATTTCTAAA16240.11348826158205712No Hit
GTACATGGGAAGCAGTGGTATCAAC15960.11153156741684922No Hit
CACTGAAGGACCTGGAATATGGCGA15600.10901581777586766No Hit
GCTTCCATGTACTCTGCGTTGATAC15360.10733865134854663No Hit
TTCCACGTCCTACAGTGGACATTTC15010.10489278364203677No Hit
TTTTTCAAGTCGTCAAGTGGATGTT14360.10035045790137563No Hit
GTATTTCTCATTTTCCGTGATTTTC14350.10028057596690391No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAA359.98692E-518.9880771
GACCTAC900.017.9331861
CGTCTTC555.904385E-717.27396810
TAGGACC28250.014.8285594
TCATGAC456.7779236E-414.7731533
GTCTTAG456.794117E-414.7685051
GGCGAAA1058.913048E-1114.4787519
GCGCCGA400.005272641314.25251919
GGCGATA400.005272641314.25251919
CGAACCT602.5645446E-514.25202116
CTTCGAA602.5670844E-514.25052513
CGCTGTA400.005277539614.250524514
CGTGGTA551.957623E-413.8201419
ACGTGGT551.957623E-413.8201418
TTGGGCT551.9616944E-413.8167564
GTAGGTC551.9651899E-413.8138573
CGCGAGA707.248318E-613.57382819
GTCGAGG2250.013.51349919
CCAACGA4050.013.37273519
AGCGAGG500.00149744813.30235319
Produced by FastQC (version 0.11.5)