##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062569_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1430985
Sequences flagged as poor quality	0
Sequence length	25
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.303268727484916	32.0	32.0	32.0	32.0	32.0
2	31.103718068323566	32.0	32.0	32.0	32.0	32.0
3	31.069410231414025	32.0	32.0	32.0	32.0	32.0
4	31.111929195623993	32.0	32.0	32.0	32.0	32.0
5	31.06335007005664	32.0	32.0	32.0	32.0	32.0
6	34.738246033326696	36.0	36.0	36.0	32.0	36.0
7	34.62123572224726	36.0	36.0	36.0	32.0	36.0
8	34.63318064130651	36.0	36.0	36.0	32.0	36.0
9	34.68186528859492	36.0	36.0	36.0	32.0	36.0
10	34.46599020954098	36.0	36.0	36.0	32.0	36.0
11	34.686982742656284	36.0	36.0	36.0	32.0	36.0
12	34.533491965324586	36.0	36.0	36.0	32.0	36.0
13	34.573762827702595	36.0	36.0	36.0	32.0	36.0
14	34.533201256477184	36.0	36.0	36.0	32.0	36.0
15	34.49545243311425	36.0	36.0	36.0	32.0	36.0
16	34.53645006761077	36.0	36.0	36.0	32.0	36.0
17	34.522059979664355	36.0	36.0	36.0	32.0	36.0
18	34.55161374857179	36.0	36.0	36.0	32.0	36.0
19	34.422847898475524	36.0	36.0	36.0	32.0	36.0
20	34.38494743131479	36.0	36.0	36.0	32.0	36.0
21	34.31160144935132	36.0	36.0	36.0	32.0	36.0
22	34.251790899275676	36.0	36.0	36.0	32.0	36.0
23	34.27823422327977	36.0	36.0	36.0	32.0	36.0
24	34.21944394944741	36.0	36.0	36.0	32.0	36.0
25	33.843023511776856	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	4.0
4	49.0
5	120.0
6	261.0
7	42.0
8	213.0
9	286.0
10	185.0
11	41.0
12	120.0
13	74.0
14	231.0
15	272.0
16	426.0
17	558.0
18	738.0
19	940.0
20	1367.0
21	1992.0
22	2955.0
23	4379.0
24	6210.0
25	8737.0
26	12268.0
27	15863.0
28	22505.0
29	30316.0
30	40169.0
31	56440.0
32	82799.0
33	123341.0
34	301516.0
35	715567.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.793842597090006	20.72036246656738	12.769706114421856	25.716088821920756
2	10.286775722879442	20.575019008430797	50.786893127893194	18.351312140796566
3	19.543972347898766	26.704670135758622	30.137352001017526	23.614005515325083
4	10.306044568945996	17.80150855622	40.42244326838937	31.470003606444635
5	9.205092875133959	41.398100902571116	37.69001635128059	11.706789871014331
6	28.739234821080245	39.26397568072846	19.956149243588435	12.040640254602861
7	25.61898168544276	31.95502129355739	23.684449759092594	18.74154726190726
8	20.863129710765303	46.91411648844796	20.32616541605815	11.896588384728588
9	26.53591132009085	15.510100441315034	21.908573850842654	36.045414387751464
10	15.420545708204653	29.87709741111147	36.74490751170884	17.957449368975038
11	32.44185721082533	19.420950994934373	31.498208838263693	16.6389829559766
12	26.954200909151982	22.778833665494773	35.8827423472244	14.38422307812885
13	31.818592086178416	24.33178633675268	26.27410316529074	17.575518411778166
14	17.50628515611607	27.70219730317298	27.272644579582007	27.51887296112894
15	18.167965634488645	43.81574712804511	22.26041389732324	15.755873340143003
16	15.960158197450175	27.800690242368397	39.22522987673444	17.013921683446988
17	16.72253743551422	33.90435568094118	33.32822554857958	16.044881334965027
18	17.232369401810118	26.356434008500585	42.40622275341164	14.004973836277657
19	24.072283187525105	25.891678784307853	27.834723304918107	22.201314723248935
20	23.539639603079202	29.771089041297174	32.010170710648325	14.679100644975307
21	18.68688671079305	28.055101299650055	26.730680012343633	26.527331977213265
22	21.789720414363735	36.177948800141095	27.345948643365414	14.686382142129755
23	15.877833640328532	35.096129621024225	31.946652819223686	17.079383919423556
24	23.439497666567995	27.878301846521826	33.51086870569925	15.171331781210922
25	19.259723174674264	31.72651953032063	33.12422151624145	15.889535778763664
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	250.0
1	250.0
2	558.0
3	866.0
4	866.0
5	866.0
6	2118.0
7	3370.0
8	3370.0
9	3370.0
10	3617.0
11	3864.0
12	3864.0
13	3864.0
14	4767.5
15	5671.0
16	5671.0
17	5671.0
18	10517.5
19	15364.0
20	15364.0
21	15364.0
22	31439.0
23	47514.0
24	47514.0
25	47514.0
26	81458.0
27	115402.0
28	115402.0
29	115402.0
30	161979.0
31	208556.0
32	208556.0
33	208556.0
34	216008.5
35	223461.0
36	223461.0
37	223461.0
38	216543.5
39	209626.0
40	209626.0
41	209626.0
42	203425.0
43	197224.0
44	197224.0
45	197224.0
46	198494.5
47	199765.0
48	199765.0
49	199765.0
50	157928.5
51	116092.0
52	116092.0
53	116092.0
54	77251.0
55	38410.0
56	38410.0
57	38410.0
58	28791.5
59	19173.0
60	19173.0
61	19173.0
62	15269.0
63	11365.0
64	11365.0
65	11365.0
66	9079.0
67	6793.0
68	6793.0
69	6793.0
70	5126.0
71	3459.0
72	3459.0
73	3459.0
74	2726.5
75	1994.0
76	1994.0
77	1994.0
78	1620.5
79	1247.0
80	1247.0
81	1247.0
82	879.5
83	512.0
84	512.0
85	512.0
86	383.0
87	254.0
88	254.0
89	254.0
90	180.0
91	106.0
92	106.0
93	106.0
94	71.0
95	36.0
96	36.0
97	36.0
98	323.5
99	611.0
100	611.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008525596005548626
2	0.002865159313340112
3	0.004402561871717733
4	0.01488485204247424
5	0.03605907818740239
6	0.047449833506291124
7	0.0681348861099173
8	0.08951875805826057
9	0.0974154166535638
10	0.1204065730947564
11	0.12194397565313403
12	0.1370384734990234
13	0.12327173240809651
14	0.12746464847639913
15	0.11495578220596303
16	0.11404731705783079
17	0.12536819044224784
18	0.13326484903755106
19	0.13550107094064578
20	0.12459948916305902
21	0.13333473097202278
22	0.1531812003619884
23	0.1454243056356286
24	0.13794693864715563
25	0.13452272385804181
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1430985.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.6808575065315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.00941258997221	29.771424174774992
2	12.061011563514393	9.08938516218829
3	3.3899393793694297	3.8320746812939794
4	1.4498303222522704	2.185233991257458
5	0.8122068756834968	1.5302325774227494
6	0.520839002656229	1.1775396145760009
7	0.368676286391932	0.9724427029500111
8	0.28859812401640605	0.8699699830171603
9	0.23389527719763584	0.7932037150381321
>10	1.5273881019429887	11.2268002187961
>50	0.16920645215696759	4.4591891391238345
>100	0.1343444287728885	10.439121453427854
>500	0.015936028140571157	4.1063275954011855
>1k	0.016677238751760512	11.869178971043306
>5k	0.0012971185695813733	3.4177967942066427
>10k+	7.412106111893561E-4	4.260079225482353
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	16295	1.1387261222165153	No Hit
GTCCTACAGTGGACATTTCTAAATT	16124	1.1267763114218527	No Hit
CTGTAGGACGTGGAATATGGCAAGA	15302	1.0693333612861071	No Hit
CTTTAGGACGTGAAATATGGCGAGG	13291	0.9288007910634981	No Hit
GTCCTACAGTGTGCATTTCTCATTT	9987	0.6979108795689682	No Hit
CTGTAGGACCTGGAATATGGCGAGA	8078	0.5645062666624737	No Hit
CTGAAGGACCTGGAATATGGCGAGA	7223	0.5047572126891616	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	6631	0.4633871074819093	No Hit
GTACATGGAAGCAGTGGTATCAACG	6000	0.4192916068302603	No Hit
ATTTAGAAATGTCCACTGTAGGACG	5986	0.4183132597476563	No Hit
TCCATGTACTCTGCGTTGATACCAC	5044	0.3524844774753055	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	4754	0.33221871647850953	No Hit
GAATATGGCAAGAAAACTGAAAATC	4127	0.2884027435647474	No Hit
GGAATATGGCGAGAAAACTGAAAAT	4072	0.28455923716880327	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	3501	0.24465665258545685	No Hit
GATATACACTGTTCTACAAATCCCG	3417	0.23878657008983323	No Hit
CTGTAGGACATGGAATATGGCAAGA	3311	0.231379085035832	No Hit
TTGTAGAACAGTGTATATCAATGAG	3273	0.22872357152590697	No Hit
ACCTGGAATATGGCGAGAAAACTGA	3235	0.226068058015982	No Hit
GACCTGGAATATGGCGAGAAAACTG	3041	0.21251096272847025	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	2976	0.20796863698780912	No Hit
GCCATATTCCACGTCCTACAGTGGA	2949	0.20608182475707293	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2935	0.20510347767446901	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	2876	0.2009804435406381	No Hit
GTCCACTGTAGGACGTGGAATATGG	2859	0.19979245065461904	No Hit
ACAGTGGACATTTCTAAATTTTCCA	2838	0.1983249300307131	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2742	0.19161626432142895	No Hit
GAAATATGGCGAGGAAAACTGAAAA	2734	0.19105720884565525	No Hit
CCATATTTCACGTCCTAAAGTGTGT	2707	0.1891703966149191	No Hit
GTGTATATCAATGAGTTACAATGAA	2649	0.1851172444155599	No Hit
ATACACACTTTAGGACGTGAAATAT	2632	0.18392925152954084	No Hit
GTGTATATCAATGAGTTACAATGAG	2560	0.17889775224757773	No Hit
CACTTTAGGACGTGAAATATGGCGA	2525	0.17645188454106786	No Hit
CCACTGTAGGACGTGGAATATGGCA	2468	0.1724686142761804	No Hit
GTTCTACAGTGTGGTTTTTATCATT	2443	0.17072156591438764	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	2427	0.1696034549628403	No Hit
ATTCCACGTCCTACAGTGGACATTT	2408	0.1682756982078778	No Hit
GAAATACACACTTTAGGACGTGAAA	2378	0.16617924017372648	No Hit
GTGTATTTCTCATTTTCCGTGATTT	2340	0.16352372666380152	No Hit
CATGTACTCTGCGTTGATACCACTG	2335	0.16317431699144294	No Hit
AGTGTGTATTTCTCATTTTCCGTGA	2175	0.15199320747596937	No Hit
CTACAGTGGACATTTCTAAATTTTC	2123	0.14835934688344043	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2052	0.14339772953594904	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2042	0.1426989101912319	No Hit
GTCCTACAGTGGACATTTCTAAATA	2026	0.14158079923968456	No Hit
GTAGGACGTGGAATATGGCAAGAAA	1974	0.13794693864715563	No Hit
GGTCCTTCAGTGTGCATTTCTCATT	1950	0.1362697722198346	No Hit
GATATACACTGTTCTACAATGCCGG	1907	0.13326484903755106	No Hit
TCCTAAAGTGTGTATTTCTCATTTT	1892	0.1322166200204754	No Hit
GTTCTACAAATCCCGTTTCCAACGA	1747	0.12208373952207745	No Hit
ACGTGGAATATGGCAAGAAAACTGA	1716	0.11991739955345443	No Hit
CCTTCAGTGTGCATTTCTCATTTTT	1644	0.1148859002714913	No Hit
CCCATGTACTCTGCGTTGATACCAC	1638	0.11446660866466106	No Hit
ATATTTCACGTCCTAAAGTGTGTAT	1633	0.11411719899230251	No Hit
TTTCTAAATATTCCACCTTTTTCAG	1628	0.11376778931994394	No Hit
ACGTCCTACAGTGGACATTTCTAAA	1624	0.11348826158205712	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1596	0.11153156741684922	No Hit
CACTGAAGGACCTGGAATATGGCGA	1560	0.10901581777586766	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1536	0.10733865134854663	No Hit
TTCCACGTCCTACAGTGGACATTTC	1501	0.10489278364203677	No Hit
TTTTTCAAGTCGTCAAGTGGATGTT	1436	0.10035045790137563	No Hit
GTATTTCTCATTTTCCGTGATTTTC	1435	0.10028057596690391	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.988193447171005E-5	0.0
10	0.0	0.0	0.0	2.0964580341513014E-4	0.0
11	0.0	0.0	0.0	2.795277378868402E-4	0.0
12	0.0	0.0	0.0	2.795277378868402E-4	0.0
13	0.0	0.0	0.0	2.795277378868402E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAA	35	9.98692E-5	18.988077	1
GACCTAC	90	0.0	17.933186	1
CGTCTTC	55	5.904385E-7	17.273968	10
TAGGACC	2825	0.0	14.828559	4
TCATGAC	45	6.7779236E-4	14.773153	3
GTCTTAG	45	6.794117E-4	14.768505	1
GGCGAAA	105	8.913048E-11	14.47875	19
GCGCCGA	40	0.0052726413	14.252519	19
GGCGATA	40	0.0052726413	14.252519	19
CGAACCT	60	2.5645446E-5	14.252021	16
CTTCGAA	60	2.5670844E-5	14.250525	13
CGCTGTA	40	0.0052775396	14.2505245	14
CGTGGTA	55	1.957623E-4	13.820141	9
ACGTGGT	55	1.957623E-4	13.820141	8
TTGGGCT	55	1.9616944E-4	13.816756	4
GTAGGTC	55	1.9651899E-4	13.813857	3
CGCGAGA	70	7.248318E-6	13.573828	19
GTCGAGG	225	0.0	13.513499	19
CCAACGA	405	0.0	13.372735	19
AGCGAGG	50	0.001497448	13.302353	19
>>END_MODULE