FastQCFastQC Report
Thu 2 Feb 2017
SRR4062569_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062569_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1430985
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA87490.6113970446929912No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA63140.44123453425437725No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC61870.43235952857647003No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA56400.39413411042044466No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA42280.2954608189463901No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA29070.20314678350926108No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA25600.17889775224757773No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA25590.178827870313106No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA24700.1726083781451238No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA24490.1711408575212179No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21950.15339084616540355No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT21560.15066545072100684No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG20990.1466821804561194No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT18130.1266959471972103No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC17590.12292232273573797No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACAC17240.12047645502922813No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG17090.11942822601215247No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA16470.11509554607490645No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA16300.11390755318888737No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACA15850.11076286613766041No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGAT15460.10803747069326372No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC15380.10747841521749006No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA14440.1009095133771493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC207.857405E-443.99968310
TAGGACC32350.027.1342724
TTGGACG508.3428895E-526.399814
ATACCGT609.755149E-625.6664836
CCGAGCC450.001398397324.4442714
TAAGACG1202.3646862E-1123.8331644
GTCCTAG3300.022.6728441
AGTACTC1950.022.563945
AGGACCT61250.022.4488185
CCTACAC3300.021.9998443
GTCGTAG602.873273E-421.99984411
GAGTTCG703.2160624E-521.99984444
CGTTTTG1800.021.99984430
GGACCTG61750.021.9107746
TGTAGGA100400.021.6061762
CTAGGAC4950.021.3331813
GGCGAAG1551.8189894E-1221.29017319
CTGTAGG98200.021.2440781
GTCCTAC89650.020.9873121
ACTGTTC15300.020.8495248
Produced by FastQC (version 0.11.5)